Multiple sclerosis (MS) is a chronic autoimmune disease, in which there is chronic inflammation leading to neurodegeneration and demyelination. To detect MS at an early stage is impossible as it includes environmental factors and genetic factors as it varies from person to person. There are various methodologies that have been developed for the treatment of this disease; however, several complications as well as obstacles have been seen which are yet to be resolved. This review describes the biomarker for MS including microRNA and vaccine as a biomarker. Some of the drugs which are under phase II clinical trials are also discussed here. Testing and continuous validation is required for improvement where MS biomarkers are brought into clinical settings.