Treatment of oxidative stress, apoptosis, and DNA injury with N-acetylcysteine at simulative pesticide toxicity in fish

Atamanalp, Muhammed; Parlak, Veysel; Özgeriş, Fatma Betül; Yeltekin, Aslı Çilingir; Uçar, Arzu; Keleş, Mevlüt Sait; Alak, Gonca

doi:10.1080/15376516.2021.1871794

Cited by 38 publications

(21 citation statements)

References 69 publications

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“…The formed yellow color enzyme activity which was obtained at the end of the DTNB (Ellman reaktifi, 5,5 0 -ditiyo-bis-2-nitrobenzoik asit) reaction was determined by reading at 412 nm absorbance [32] MDA: lipid peroxidation μmol/g tissue Obtained by determining the level of MDA (malondialdehyde), a product of lipid peroxidation from the breakdown of polyunsaturated fatty acids (the pink colored complex formed by thiobarbituric acid and MDA at 95 C, wavelength of 532 nm spectrophotometric measurement). [33] 8-OHdG: DNA damage ng/ml For determining the DNA damage level in fish brain the 8-OHdG process was performed according to provider's guide (Catalog No: 201-00-0041/SunRed) Caspase À3 activity: apoptosis ng/ml Caspase-3 level (CASP3 ELISA Kit (Catalog No: 201-00-0031) (SunRed brand) was performed according to provider's guide.…”

Section: Ache: Acetylcholinesterase U/mlmentioning

confidence: 99%

May PdCu@f‐MWCNT NPs be an ecotoxicologic risk?

Alak

Yeltekin

Köktürk

et al. 2023

Applied Organom Chemis

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The mechanisms of various metal nanoparticles (NPs) are still a matter of debate in aquatic toxicology. In order to close this gap, the number of studies on this subject is increasing. The aims of this study are to explore the interactions of PdCu@f-MWCNT NPs with zebrafish and get to know if it has an ecotoxicological risk. In this study, we synthesized, characterized PdCu@f-MWCNT NPs, and investigated its ecotoxicological effects. The chemical and morphological structures of PdCu@f-MWCNT nanomaterials were elucidated with advanced analysis techniques such as scanning electron microscope (SEM), X-ray spectrophotometer (EDX), transmission electron microscope (TEM), and X-ray diffraction (XRD) analyses. The ecotoxicological risks were investigated by conducting biochemical analyses and malformation defects in zebrafish. The results were obtained using multiple marker applications including oxidative stress parameters, proinflammatory cytokine expressions, DNA damage, and apoptosis markers. The obtained findings showed embryonic development, survival rate, and incidence of malformation increasing in parallel with the dose increase in zebrafish exposed to PdCu@f-MWCNT NPs at 10-1000 μg/L levels. Despite the observed decreases in antioxidant enzyme activities, GSH level, AChE activity, and NRF-2 level, inductions in malondialdehyde (MDA) level, IL-6, TNF-α, NF-kB, DNA damage, and apoptosis levels were observed with increasing dose. Considering these findings, it has been determined that there are toxic effects in zebrafish at all levels above the concentration that we examine as a safe dose (10 μg/L). The effects of all studied concentrations of this NP above the safe dosage, especially on oxidative stress, malformation, and immune system, were found to be quite significant. The more widespread use of nanotechnology in the future will mean more contact of aquatic organisms with NPs. As a result, it is clearly seen that these organisms, which form the important link of the food chain, and the aquatic environment are living at risk conditions.

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Section: Ache: Acetylcholinesterase U/mlmentioning

confidence: 99%

May PdCu@f‐MWCNT NPs be an ecotoxicologic risk?

Alak

Yeltekin

Köktürk

et al. 2023

Applied Organom Chemis

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“…Arguably, the most important finding of this study is that exposure to high temperature (32 C for 4 weeks) and pesticides causes marked increases in cellular apoptosis ($1.2-1.5-fold) and CAS (a protease enzyme that plays an essential role in PCD; Takle & Andersen, 2007) mRNA levels ($2.4-7-fold), which were accompanied by a significant increase in DNP ($1.1-1.4-fold) and NTP ($1.7-2.2-fold) expressions in the kidney of goldfish. Similarly, chronic exposure to cypermethrin (2.05 Â 10 À3 mg/L for 2 weeks), a synthetic pyrethroid used as an insecticide, caused a marked elevation of CAS activity ($1.2-fold) and the oxidative biomarker MDA ($5-fold) in the kidney, gill, and liver tissues of rainbow trout (Atamanalp et al, 2021). Interestingly, this same compound (dose: 3 μg/L for 3 days of treatment) markedly elevated CAS-3 and -9 transcript levels, as well as increased NO content in zebrafish embryos (Jin, Zheng, & Fu, 2011).…”

Section: Interactive Effects Of Heat and Pesticides Exposure On Cellu...mentioning

confidence: 99%

Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation

Lacy

Rahman

2022

J of Applied Toxicology

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One of many noteworthy consequences of increasing societal reliance on pesticides is their predominance in aquatic environments. These pernicious chemicals interact with high temperatures from global climate change, heat waves, and natural variations to create unstable environments that negatively impact organisms' health.To understand these conditions, we examined the dose-dependent effects of environmentally relevant pesticide mixtures (metolachlor, linuron, isoproturon, tebuconazole, aclonifen, atrazine, pendimethalin, and azinphos-methyl) combined with elevated temperatures (22 control vs. 32 C for 4-week exposure) on renin, dinitrophenyl protein (DNP, an indicator of reactive oxygen species, ROS), 3-nitrotyrosine protein (NTP, an indicator of reactive nitrogen species, RNS), superoxidase dismutase (SOD, an antioxidant), and catalase (CAT, an antioxidant) expressions in the kidneys of goldfish (Carassius auratus). Histopathological analysis showed widespread damage to kidney tissues in high temperature and pesticide co-exposure groups, including rupture of the epithelial layer, hemorrhaging, and degeneration of tubular epithelium. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical analyses demonstrated significant declines in renin receptorlike mRNA and protein expressions in kidney tissues under combined exposure to high temperature and pesticides compared with controls; conversely, expression of DNP, NTP, SOD, and CAT increased in kidney tissues under the same conditions. Apoptotic cells were also increased in co-exposure groups as assessed by in situ terminal deoxynucleotidyl transferase dUTP nick labeling (TUNEL) assay. The enhanced apoptosis in kidneys of heat and pesticides co-exposed fish was associated with increased caspase-3 (a protease enzyme) mRNA levels. Our results demonstrated that high temperature and pesticides induced oxidative/nitrative stress (i.e., ROS/RNS), damaged tissues, increased cellular apoptosis, and suppressed renin expression in kidneys of goldfish.

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“…In vitro and in vivo studies on NM in fish had shown that NPs can induce oxidative stress by triggering the ROS formation. Stimulation of oxidative stress causes extensive damage to biomolecules such as DNA, proteins, lipids and cellular membranes [48]. Oxidative stress in the cellular system is produced as a result of an imbalance between oxidative and reducing processes and is typically induced when the physiological antioxidant defense system is unable to counteract the increased level of ROS [35].…”

Section: Exposure and Potential Pathways To Toxicity Of Nanoparticles...mentioning

confidence: 99%

Catfish as an Ecotoxicological Model for Assessment of Nanoparticle Toxicity Profiling

Atamanalp¹,

Uçar²,

Alak³

2022

Catfish - Advances, Technology, Experiments

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The developing technological infrastructure has accelerated the evolution of nanoscience and encouraged the use of nanomaterials in very large areas. However, environmental liberation of nanomaterials can pose potential risks. Although different toxicity screening methodologies have been successfully used to assess the potential risks of these substances, little is known about their environmental impact. Fish are the most visible members of the aquatic ecosystem, vulnerable to toxicants. Although the ecotoxicology of fish and nanoparticles are complex, this review evaluates approaches to using catfish as a bio-indicator for the effects of nanoparticles on fish.

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Treatment of oxidative stress, apoptosis, and DNA injury with N-acetylcysteine at simulative pesticide toxicity in fish

Cited by 38 publications

References 69 publications

May PdCu@f‐MWCNT NPs be an ecotoxicologic risk?

May PdCu@f‐MWCNT NPs be an ecotoxicologic risk?

Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation

Catfish as an Ecotoxicological Model for Assessment of Nanoparticle Toxicity Profiling

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