Treatment of pernicious anemia with crystalline vitamin B12

Reisner, Edward H.

doi:10.1016/0002-9343(49)90138-2

Cited by 25 publications

(4 citation statements)

References 12 publications

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“…Our findings amplify earlier reports on the effect of vitamin B12 given parenterally in pernicious anaemia (West, 1948;Ungley, 1948aUngley, , 1948bUngley, , 1949Hall and Campbell, 1948;Spies, Stone, Kartus, and Aramburu, 1948;Berk, Denny-Brown, Finland, and Castle, 1948;Bethell, Meyers, and Neligh, 1948 ;Spies, Suarez, Garcia Lopez, Milanes, Stone, Lopez Toca, Aramburu, and Kartus, 1949;West and Reisner, 1949 160 ,ug. Conclusions are based on the means of a number of responses at each dose level. Reticulocyte responses were compared with the "expected " response to liver extract according to Isaacs and Friedman (1938), but a better yardstick was the increase of red blood cells in 15 days.…”

Section: Neurological Manifestationssupporting

confidence: 90%

Vitamin B12 in Pernicious Anaemia

Ungley¹

1949

BMJ

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Section: Neurological Manifestationssupporting

confidence: 90%

Vitamin B12 in Pernicious Anaemia

Ungley¹

1949

BMJ

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“…Morphologically, the criteria stated by W introbe (53) were used to classify megaloblasts. However, no attempt was made to evaluate in the autoradiograms pro megaloblasts separately from basophilic megaloblasts, and no distinction was made between early and late polychromatophilic forms.…”

Section: Methodsmentioning

confidence: 99%

“…Its application permitted Rickes et al (38) to isolate in 1948 the cristalline substance of B12 from the liver. Subsequently, the parenteral route became the application of choice (53) and synthe tic products were available after knowledge of the vitamin's structure in 1955 (6, 17).…”

mentioning

confidence: 99%

Pernicious Anaemia

Schmid¹,

Moeschlin²,

Haegi³

1964

Acta Haematol

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“…Shortly thereafter, both groups discovered that the compound contained cobalt [29,30]. The potency of the purified vitamin B 12 as the anti-pernicious factor was tested by several groups and found to be outstandingly high, with doses as small as 4 µg giving a full reticulocyte response [31,32]. …”

Section: Isolation Crystallization and Synthesis Of The ‘Extrinsic’ mentioning

confidence: 99%

The Discovery of Vitamin B12

Scott¹,

Molloy

2012

Ann Nutr Metab

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The discovery of vitamin B₁₂, the elucidation of its role in metabolism, and the effects and treatment of its deficiency occurred in distinct phases over more than 100 years, and it was the subject of two separate Nobel Prizes. The valuable contribution of clinical reports and studies of patients with pernicious anemia throughout the 19th century resulted in enough clinical definition to allow Minot and Murphy to put together the first hallmark study on treatment of the condition, leading them to a Nobel Prize. These researchers were not the first to suggest that an inadequacy of nutrients was the cause of pernicious anemia, but their particular input was a carefully designed intervention in well-characterized pernicious anemia patients, of a special diet containing large amounts of liver. They found consistent improvement in the clinical and blood status of all subjects, most of whom remained on remission indefinitely. After the successful intervention studies, the next advance was made by Castle who discovered that a gastric component, which he called intrinsic factor, was missing in pernicious anemia. Many years later, intrinsic factor was found to be a glycoprotein that formed a complex with vitamin B₁₂, promoting its absorption through ileal receptors. The vitamin was isolated by two groups simultaneously and was crystallized and characterized in the laboratory of Dorothy Hodgkin, contributing to her Nobel Prize in 1964. Subsequently, the various biochemical roles of vitamin B₁₂ were elucidated, including its important interaction with folate and their common link with megaloblastic anemia. Many of the early clinical studies recognized that vitamin B₁₂ deficiency also caused a severe neuropathy leading to paralysis and death, while post mortem analysis demonstrated spinal cord demyelination. Vitamin B₁₂ is still the subject of intense research and, in particular, its role in preventing these irreversible neurological lesions remains unclear.

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Treatment of pernicious anemia with crystalline vitamin B12

Cited by 25 publications

References 12 publications

Vitamin B12 in Pernicious Anaemia

Vitamin B12 in Pernicious Anaemia

Pernicious Anaemia

The Discovery of Vitamin B12

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