Treatment of Picea abies and Pinus sylvestris Stumps with Urea and Phlebiopsis gigantea for Control of Heterobasidion

Kärhä, Kalle; Koivusalo, Ville; Palander, Teijo; Ronkanen, Matti

doi:10.3390/f9030139

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…Community differences between asymptomatic and symptomatic Heterobasidion -infected plant anatomies have also been studied ( 15 , 16 ). Antagonistic agents against Heterobasidion have also been broadly identified, providing microbial and mycobiota perspectives ( 17 , 18 ). However, most of the studies have shown the fragment of a single kingdom in the decay process, which might not be a true reflection of the full dynamics of the interaction within the microbiome community assembly.…”

Section: Introductionmentioning

confidence: 99%

Interkingdom and intrakingdom interactions in the microbiome of Heterobasidion fruiting body and associated decayed woody tissues

Ren,

Penttilä,

Kasanen

et al. 2023

Appl Environ Microbiol

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The Heterobasidion annosum species complex is a major threat to the forest industry in the northern temperate regions of the world. The fungal and bacterial biota communities in Heterobasidion -infected trees have been extensively studied, but less is known about the interkingdom and intrakingdom interactions among the microbiome. The bacteria-fungi interkingdom analysis has revealed significant findings compared to the intrakingdom analysis in recent studies. In this study, we aimed to (i) unravel the contribution and development of the bacteria and other fungi in the Heterobasidion -infected wood decay process; (ii) uncover how environmental factors and the conifer pathogen drive the microbiome community structure and function changes; and (iii) identify the core microbiome during the Heterobasidion -mediated wood decay process. Internal transcribed spacer and 16S amplicon sequencing data from the Heterobasidion fruiting body and its associated woody tissue were applied in this analysis. Samples collected from managed forest and nature reserve forest were classified into four decay classes based on the extent of decay of the wood. Our result showed that bacteria and fungi have different abundances and activities in both study materials. The Heterobasidion ecological correlation network is conserved, especially in the fruiting body. The core microbiome defined in our analysis showed huge potential for the microbiome community’s functioning. A particular Heterobasidion Operational Taxonomic Units (OTU) had the most important impact on the community and disease development. Forest management has the biggest impact on the microbiome assembly, and the fungal community is more sensitive to environmental changes. IMPORTANCE We applied macro- (forest stand and forest management) and micro-scale (bacterial and fungal community) analyses for a better understanding of the Heterobasidion pathosystem and associated wood decay process. The core microbiome, as defined by hierarchy analysis and a consistent model, and environmental factors correlation with the community assembly were found to be novel.

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Section: Introductionmentioning

confidence: 99%

Interkingdom and intrakingdom interactions in the microbiome of Heterobasidion fruiting body and associated decayed woody tissues

Ren,

Penttilä,

Kasanen

et al. 2023

Appl Environ Microbiol

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“…Heterobasidion root rot caused by Heterobasidion annosum species complex is one of the most economically significant fungal diseases in the Northern Hemisphere . For prevention of spore infection of freshly cut stumps, stump surfaces can be treated with biological or chemical preparations (Kärhä et al, 2018). The Finnish BCA (biological control agent) Rotstop® containing oidia of Phlebiopsis gigantea is registered in Latvia and has been widely used since 2008 (Kenigsvalde et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Saprobic Basidiomycetes (Bjerkandera adusta, Phlebiopsis gigantea and Sistotrema brinkmannii) Using Different Biological Waste Substrates

Burņeviča,

Kļaviņa,

Ciseļonoka

et al. 2023

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Development of new biological preparations to control Heterobasidion root rot is a complex process, but when a potential antagonist is identified, cultivation of the fungus is required. In this study, five different substrates (deciduous sawdust, coniferous sawdust, rye bran, straw and corn kernels) were tested as substrates for the cultivation of three fungal species: Bjerkandera adusta, Phlebiopsis gigantea, and Sistotrema brinkmannii, which could be potentially used against Heterobasidion spp. Mycelial growth was evaluated visually, and oidia production was estimated microscopically. In the straw substrate, P. gigantea produced significantly more (p < 0.05) oidia compared to the other substrates. In addition, oidia production at two different incubation temperatures were compared. As a result, the best substrate for cultivation of all three fungal species was coniferous sawdust.

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“…Recently, Heterobasidion root rot has been found in pine trees in central Finland, which was previously solely common in southern Finland (Piri et al, 2021 ). Stump treatment using urea or Phlebiopsis gigantea is commonly used to prevent Heterobasidion infection (Kärhä et al, 2018 ). However, the prospect of using resistant genotypes obtained through breeding is a potential and durable approach to manage root and stem rot diseases.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Transcriptome and Terpene Constituents of Scots Pine Genotypes Inherently Resistant or Susceptible to Heterobasidion annosum

et al. 2022

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Root and stem rot caused by Heterobasidion annosum is a severe problem in boreal Scots pine. Dissecting the features of disease resistance is generally an essential step in resistance breeding in plants and forest trees. In this study, we explored inherent resistance factors of Scots pine against H. annosum. A total of 236 families consisting of 85 full-sib (FS), 35 half-sib population mix (HSpm), and 116 half-sib (HS) families of Scots pine seedlings were inoculated with a H. annosum isolate. We sampled needle tissues before inoculation for terpene measurements and RNA sequencing. Based on the lesion area, the extremes of 12 resistant and 12 susceptible families were selected for further analyses. Necrotic lesions resulting from fungal infection were in a weak to moderate relationship with the plant height. Monoterpenes were the principal terpene compounds observed in Scots pine seedlings. Concentrations of 3-carene were significantly higher in pine genotypes inherently resistant compared with susceptible seedlings. By contrast, susceptible genotypes had significantly higher proportions of α-pinene. Gene ontology analysis of differential expressed transcripts (DETs) revealed that response to biotic factors was enriched in resistant seedlings. Functional characterization of individual DETs revealed that higher expression of transcripts involved in response to abiotic stress was common in susceptible genotypes. This observation was supported by the annotation of hub genes in a key module that was significantly correlated with the lesion trait through weighted gene co-expression network analysis (WGCNA) of 16 HS and HSpm samples. These findings contribute to our understanding of constitutive resistance factors of Scots pine against Heterobasidion root and stem rot diseases.

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Treatment of Picea abies and Pinus sylvestris Stumps with Urea and Phlebiopsis gigantea for Control of Heterobasidion

Cited by 12 publications

References 36 publications

Interkingdom and intrakingdom interactions in the microbiome of Heterobasidion fruiting body and associated decayed woody tissues

Interkingdom and intrakingdom interactions in the microbiome of Heterobasidion fruiting body and associated decayed woody tissues

Cultivation of Saprobic Basidiomycetes (Bjerkandera adusta, Phlebiopsis gigantea and Sistotrema brinkmannii) Using Different Biological Waste Substrates

Analysis of Transcriptome and Terpene Constituents of Scots Pine Genotypes Inherently Resistant or Susceptible to Heterobasidion annosum

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