Treatment of skin defects with PRP enriched with hyaluronic acid – histological aspects in rat model

Abstract

Tissue healing is a complex, dynamic process, characterized by the replacement of devitalized and absent cell and tissue structures. This can be obtained by different methods, these being found in the "reconstructive scale", which although it is very rich does not offer a universally valid solution for closing skin wounds. In plastic surgery, platelet-rich plasma (PRP) has proven effective in the treatment of skin graft donor areas, burn wounds, skin grafts, tendons, or varicose ulcers. Also, hyaluronic acid (HA) has found its utility in different areas of medicine, other than the esthetics field, with satisfactory results after its use in various lesions. The aim of our study was to find a method of healing wounds with skin defect that shortens the time of complete epithelialization compared to native healing, which is accessible to any patient both by its simplicity and by the lowest possible costs. So, we decided to test a preparation consisting of PRP and granular HA in this type of wounds on a group of 30 Wistar rats. Corroborating the macroscopic data with the microscopic ones, an important similarity can be observed between the healing of the adjuvant-treated lesion at 14 days postoperatively and the healing of the lesion left to natural healing at 21 days, thus shortening the healing period by seven days.

How antidepressants affect the cerebral ischemic injury and ischemic stroke

“…The neuroprotective effects of trazodone have been reported by previous studies. Trazadone improved depressive disorder and transient global ischemia in animal models [20]. Trazadone could also exhibit neuroprotective effect during inflammation and prevent injury of nerve cells in mice with cerebral ischemia [21].…”

Evaluation of Extracellular Matrix Remodeling in Full-thickness Skin Grafts in Mice

“…Furthermore, exogenous HA administration has been shown to promote tissue repair in rodent skin and tendons by regulating collagen turnover, thus contributing to remodeling of the ECM. 12 The activation of fibroblasts is accompanied by the release of active TGF-β, an important inducer of collagen production, ECM remodeling, and fibrosis. 13,14 These cells have been shown by us and others to enhance HA synthesis in cell culture.…”

“…Furthermore, exogenous HA administration has been shown to promote tissue repair in rodent skin and tendons by regulating collagen turnover, thus contributing to remodeling of the ECM. 12 …”