“…After the acclimatization of seed culture, the dilution ratio was gradually decreased from 1:10 to 1:3 to increase organic loading rate (from about 2 to 17 kg TCOD/m 3 -day) and to study the effects of different feed strengths on the digestion performance of the reactor. Since molasses wastewater is characterized by moderately acidic [4], the pH of the feed wastewater was adjusted by the gradual addition of NaHCO 3 (Merck Chemical Corp.), as similarly conducted by Gohil and Nakhla [40]. A nutrient solution/basal media containing essential micro and macro nutrients for an optimum anaerobic microbial growth was also prepared with the following components, and added 1 mL/L of the daily fed subtrate at relatively high organic loading conditions [41]: 5 g/L MgSO 4 ·7H 2 O, 6 g/L FeCl 2 ·6H 2 O, 10 g/L CoCl 2 ·6H 2 O, 1 mg/L H 3 BO 3 , 1 mg/L ZnSO 4 ·7H 2 O, 1 mg/L CuSO 4 ·5H 2 O, 100 mg/L MnCl 2 ·6H 2 O, 1 mg/L (NH 4 ) 6 Mo 24 ·4H 2 O, 585 mg/L Al 2 (SO 4 ) 3 ·18H 2 O, and 1 g/L Na 2 SiO 3 ·9H 2 O.…”