Treatment of Type I Diabetes using Autologous Adipose Derived Mesenchymal Stem Cells Translated to Insulin Secreting Islet like Cell Aggregates

Thadani, Jaymesh; Marathe, Anant; Vakodikar, Shivani; Kshatriya, Prashant; Modi, Dhwani; Vyas, Rajni

doi:10.17659/01.2017.0064

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…Clinical case studies using autologous treatment of adipose-derived MSCs are still very limited and challenging because of the deleterious effect of DM on ad-MSCs as mentioned above [3]. Interestingly, a clinical case study suggests autologous Ad-MSCs translated to insulin-secreting islet-like cell aggregates (ICAs) as an effective alternative strategy for the treatment of juvenile diabetes where a 21-yearold female with type 1 diabetes had received treatment with ICAs derived from autologous Ad-MSCs and the patient's insulin requirement was significantly reduced with no side effects were observed [57].…”

Section: Discussionmentioning

confidence: 99%

The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells

Badr,

Kamal,

El-Maraghy

et al. 2024

Biol Res

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Background Diabetes mellitus (DM) is a global epidemic with increasing incidences. DM is a metabolic disease associated with chronic hyperglycemia. Aside from conventional treatments, there is no clinically approved cure for DM up till now. Differentiating mesenchymal stem cells (MSCs) into insulin-producing cells (IPCs) is a promising approach for curing DM. Our study was conducted to investigate the effect of DM on MSCs differentiation into IPCs in vivo and in vitro. Methods We isolated adipose-derived mesenchymal stem cells (Ad-MSCs) from the epididymal fat of normal and STZ-induced diabetic Sprague–Dawley male rats. Afterwards, the in vitro differentiation of normal-Ad-MSCs (N-Ad-MSCs) and diabetic-Ad-MSCs (DM-Ad-MSCs) into IPCs was compared morphologically then through determining the gene expression of β-cell markers including neurogenin-3 (Ngn-3), homeobox protein (Nkx6.1), musculoaponeurotic fibrosarcoma oncogene homolog A (MafA), and insulin-1 (Ins-1) and eventually, through performing glucose-stimulated insulin secretion test (GSIS). Finally, the therapeutic potential of N-Ad-MSCs and DM-Ad-MSCs transplantation was compared in vivo in STZ-induced diabetic animals. Results Our results showed no significant difference in the characteristics of N-Ad-MSCs and DM-Ad-MSCs. However, we demonstrated a significant difference in their abilities to differentiate into IPCs in vitro morphologically in addition to β-cell markers expression, and functional assessment via GSIS test. Furthermore, the abilities of both Ad-MSCs to control hyperglycemia in diabetic rats in vivo was assessed through measuring fasting blood glucose (FBGs), body weight (BW), histopathological examination of both pancreas and liver and immunoexpression of insulin in pancreata of study groups. Conclusion Our findings reveal the effectiveness of N-Ad-MSCs in differentiating into IPCs in vitro and controlling the hyperglycemia of STZ-induced diabetic rats in vivo compared to DM-Ad-MSCs. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells

Badr,

Kamal,

El-Maraghy

et al. 2024

Biol Res

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show abstract

“…Numerous studies have described the beneficial hypoglycemic effect of taurine on increasing insulin availability, through the activation of hepatic glucose accumulation as glycogen (21) and the inhibition of gluconeogenesis in animal models of diabetes (95). The positive effect of taurine on glucose regulation has also been confirmed via a more prominent formation of islet-like cell aggregates (ICAs) from autologous adipose-derived mesenchymal stem cells (ADMSCs), following the administration of taurine (96).…”

Section: Effects Of Taurine On Type 2 Diabetesmentioning

confidence: 99%

Ameliorative effect of taurine against diabetes and renal‑associated disorders (Review)

Baliou

Adamaki

Ιωάννου

et al. 2021

Med Int

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To develop novel therapeutic methods for both diabetic and renal disorders, scientists had initially focused on elucidating the molecular mechanisms of taurine in established cell lines and mouse models. Although a large amount of data have been revealed, taurine has been confirmed to be the next step of novel promising therapeutic interventions against diabetic disorders. Taurine appears to ameliorate diabetes 1-related complications in various organs through its antioxidant, anti-inflammatory and anti-hormonal actions. In type 2 diabetes, taurine has been positively implicated in glucose homeostasis, exerting potent hypoglycemic, anti-obesity, hypotensive and hypolipidemic effects. Of particular interest is that taurine provides protection against renal dysfunction, including hypertension and proteinuria, specific glomerular and tubular disorders, acute and chronic renal conditions, and diabetic nephropathy. The ameliorative effects of taurine against renal disorders are based on its osmoregulatory properties, its association with signaling pathways and its association with the renin-angiotensin-aldosterone system (RAAS). Further clinical studies are required to ensure the importance of research findings. Types of diabetesDiabetes is a prevalent endocrine disease associated with oxidative stress. In 2014, 422 million individuals were diagnosed with diabetes worldwide, while diabetes was directly associated with 1,5 million deaths in 2012 and 2,2 million deaths indirectly through an increased risk of cardiovascular mortality and other diseases (1).Diabetes mellitus is categorized into two types according to insulin dependence. Type 1 diabetes mellitus or insulin-dependent diabetes mellitus (IDDM) (formerly known as juvenile diabetes) is characterized by hyperglycemia and hypoinsulinemia. Type 1 diabetes mellitus is considered an autoimmune disease, in which T-cells mediate the elimination of pancreatic β-cells and thereby contribute to the production of low insulin levels (2). In type 2 diabetes mellitus or non-insulin dependent diabetes mellitus (NIDDM) (formerly known as adult diabetes), insulin resistance seems to be the predominant factor and occurs from defects in insulin secretion and a low tissue sensitivity to insulin (3). Diabetes is also known to cause complications, such as cardiovascular diseases, neuropathy, nephropathy, retinopathy, foot ulcers, skin lesions and hearing impairment (4).Diabetes mellitus is associated with high blood sugar levels for a long period of time due to alterations in carbohydrate, protein and fat metabolism, which results from a dysfunction

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Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats

Sayed

Salem

Abdallah³

et al. 2019

Comp Clin Pathol

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Treatment of Type I Diabetes using Autologous Adipose Derived Mesenchymal Stem Cells Translated to Insulin Secreting Islet like Cell Aggregates

Cited by 4 publications

References 13 publications

The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells

The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells

Ameliorative effect of taurine against diabetes and renal‑associated disorders (Review)

Clinicopathological studies on the use of laser-activated adipose-derived stromal vascular fraction in treatment of streptozotocin-induced diabetes in rats

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