Treatment options for neonatal infections in the post-cefotaxime era

Franco, Susannah; Rampersad, Daniel; Mesa, Daniel; Hammerschlag, Margaret R.

doi:10.1080/14787210.2022.2110069

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Whilst there could be pharmaceutical formulations able to chelate out calcium, this may be physiologically harmful to the eye. In the case of new-borns up to 28 days old, ceftriaxone is contraindicated due to the potential need for calcium treatment, which can be required in premature infants and those with impaired bilirubin binding [148][149][150][151]. Thus, although further investigations may be able to circumvent these issues with ceftriaxone, alternatives are needed.…”

Section: Log Reductionmentioning

confidence: 99%

A novel ex vivo bovine corneal infection and clearance model for Neisseria gonorrhoeae, Staphylococcus aureus, and Pseudomonas aeruginosa

Ukachukwu

Alany

Snyder

2023

Preprint

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Ocular infections caused by bacterial pathogens may damage the cornea and rapidly progress to permanent blindness. Topical application of an ophthalmic formulation is often used to treat corneal infections. The animal models used in many preclinical studies frequently involve expensive in vivo experiments that compromises the corneal epithelium to mimic real life conditions such as during contact lens wear, but these do not consider other instances where infection occurs in intact corneas such as in ophthalmia neonatorum. To develop an ex vivo model of infection, bovine eyes from human food chain waste were processed and the corneas inoculated with Neisseria gonorrhoeae strain NCCP 11945, Staphylococcus aureus strain 6571, and Pseudomonas aeruginosa strain ATCC 15442 for 1 hour, 4 hours, and 6 hours, respectively. Inoculation included intact bovine corneas and those compromised with scalpel, needle, and blot methods. Recovery of N. gonorrhoeae, S. aureus, and P. aeruginosa colonies demonstrated that infection of bovine corneas was achieved with intact and compromised corneas using this model. In addition, corneas inoculated with the bacteria were treated with a suitable antibiotic, demonstrating clearance of the bacterial infections with at least 5 log10 reduction. This model is appropriate for both establishing infection and testing the ability of antimicrobial agents to clear bacterial eye infections. The bovine ex vivo model is reliable, cost-effective, suitable for different bacteria species, and reduces the need for further animal exploitation in laboratory research.

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Section: Log Reductionmentioning

confidence: 99%