Treatment with Lactobacillus delbruckii inhibits hypertrophic scar fibrosis

Abstract

Hypertrophic scar (HS) is a common complication of skin after burn wounds and is a serious fibrosis disease which lack effective treatments. Scholars have suggested that certain probiotics may significantly impact on fibrosis. Hypertrophic scar derived fibroblasts (HFBs) was treated with or without Lactobacillus delbruckii. The fibrosis was determined under in vivo and in vitro conditions. The results showed that L. delbruckii significantly decreased expression of Collagen I, Collagen III and α-SMA in hypertrophic scar derived fibroblasts (HFBs). Masson and Sirius-red staining results indicated that L. delbruckii significantly reduced both Collagen I and Collagen III expression and inhibited scar hyperplasia in rabbit ear model and L. delbruckii inhibited the proliferation of HFBs. To further assess the mechanism of L. delbruckii inhibit hypertrophic scar fibrosis, ribonucleic acid sequencing and iTRAQ technology was used to analysis HFBs treat with or without L. delbruckii. Results revealed that L. delbruckii exert its inhibiting fibrosis effect by targeting multiple pathways at both the proteome and transcriptome levels. L. delbruckii decreased the fibrosis of hypertrophic scar which may provide pharmacological evidence to aid in its clinical application.