Treatment With Platelet Lysate Inhibits Proteases of Synovial Fluid in Equines With Osteoarthritis

Perrone, Gustavo; Lastra, Yael; Gonzalez, Camilo Martinez; Caggiano, Nicolás; Giménez, Rubén; Pareja, Román; Simone, Emilio De

doi:10.1016/j.jevs.2020.102952

Cited by 13 publications

(18 citation statements)

References 46 publications

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“…Treatment with systemic non-steroidal anti-inflammatories and intra-articular therapies, including steroids and lubricants, are mainstays of treatment for synovitis and early osteoarthritis (OA) as these drugs interrupt the inflammatory cascade and provide viscosupplementation [ 1 , 3 – 5 ]. Biologic therapies, such as platelet rich plasma (PRP) or PRP lysate, are also increasingly being used [ 4 , 6 , 7 ]. Early treatment of synovitis is critical to slow the progression of OA by combating inflammation-induced changes in synovial fluid composition and viscosity.…”

Section: Introductionmentioning

confidence: 99%

Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models

et al. 2021

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Background Lameness is a debilitating condition in equine athletes that leads to more performance limitation and loss of use than any other medical condition. There are a limited number of non-terminal experimental models that can be used to study early inflammatory and synovial fluid biophysical changes that occur in the equine joint. Here, we compare the well-established carpal IL-1β-induced synovitis model to a tarsal intra-articular lavage model, focusing on serial changes in synovial fluid inflammatory cytokines/chemokines and the synovial fluid lubricating molecules lubricin/proteoglycan 4 and hyaluronic acid. The objectives of this study were to evaluate clinical signs; synovial membrane and synovial fluid inflammation; and synovial fluid lubricants and biophysical properties in response to carpal IL-1β synovitis and tarsal intra-articular lavage. Results Hyaluronic acid (HA) concentrations, especially high molecular weight HA, and synovial fluid viscosity decreased after both synovitis and lavage interventions. Synovial fluid lubricin concentrations increased 17–20-fold for both synovitis and lavage models, with similar changes in both affected and contralateral joints, suggesting that repeated arthrocentesis alone resulted in elevated synovial fluid lubricin concentrations. Synovitis resulted in a more severe inflammatory response based on clinical signs (temperature, heart rate, respiratory rate, lameness and joint effusion) and clinicopathological and biochemical parameters (white blood cell count, total protein, prostaglandin E2, sulfated glycosaminoglycans, tumor necrosis factor-α and CC chemokine ligands − 2, − 3, − 5 and − 11) as compared to lavage. Conclusions Synovial fluid lubricin increased in response to IL-1β synovitis and joint lavage but also as a result of repeated arthrocentesis. Frequent repeated arthrocentesis is associated with inflammatory changes, including increased sulfated glycosaminoglycan concentrations and decreased hyaluronic acid concentrations. Synovitis results in more significant inflammatory changes than joint lavage. Our data suggests that synovial fluid lubricin, TNF-α, CCL2, CCL3, CCL5, CCL11 and sGAG may be useful biomarkers for synovitis and post-lavage joint inflammation. Caution should be exercised when performing repeated arthrocentesis clinically or in experimental studies due to the inflammatory response and loss of HA and synovial fluid viscosity.

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Section: Introductionmentioning

confidence: 99%

Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models

et al. 2021

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“…Perrone et al [20] prepared the PL with 8 mL of blood collected in a tube containing 2 mL of trisodium citrate as anticoagulant. Blood samples were first centrifuged for 10 min at 1000g.…”

Section: Pl Preparationmentioning

confidence: 99%

Platelet lysate for the treatment of osteoarthritis: a systematic review of preclinical and clinical studies

Valtetsiotis,

Di Martino,

Brunello

et al. 2024

Musculoskelet Surg

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Intra-articular injection-based therapy is often used aside conservative treatment and lifestyle modifications to manage knee osteoarthritis (KO) patients. Conventional injections contain steroids and hyaluronic acid, while more recently multipotential adult stem cell, platelet-rich plasma (PRP), and platelet lysate (PL) injections have been used to promote cartilage regeneration or repair. The aim of the current study is to analyse current evidence on PL injections for the treatment of KO and to determine if these are effective and how these perform compared to other injection regimens. The databases of Scopus, Embase, PubMed, Web of Science, and Cochrane Library were searched on 30 June 2023. Risk of bias was assessed using the SYRCLE tool for animal studies and Cochrane RoB 2 as well as ROBINS-I tool for human studies. Studies were included if these were in English, any year, and regarded animals with osteoarthritis (OA) or human adult patients with OA. In vitro trials and non-adult human studies were excluded. Results on OA symptom stage and severity, and pain were recorded. The research retrieved three human studies (n = 48, n = 25, n = 58) and four animal studies: one rabbit, two studies, and one rat study. PL was found to decrease KO symptoms at follow-up ≤ 1 year with respect to baseline levels and when compared to hyaluronic acid or platelet-rich plasma. Symptoms returned 6 months–1 year after the final administration, with studies showing peak efficacy at approximately 6 months. Animal studies showed clinical improvements, reduction of lameness, and partial effect on the cartilage regeneration of the seven studies, two had a high risk of bias, four were associated to some concerns, and one had low risk. A major source of bias in these studies was the use of questionnaires and scoring that could be subject to interpretation. Overall, PL was well-tolerated and showed efficacy comparable to PRP; when pain control was assessed, it showed similar efficacy compared to hyaluronic acid. These findings may support its use in clinical trials to confirm these initial findings; future research should also focus on the comparison with other non-surgical treatments, on a more detail of the potential regenerative properties, and to optimise the treatment schedule.

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“…Interest in using platelet lysate (PL) instead of PRP has been increasing in equine clinical practice [45,46], since, among the advantages, it can be long-term stored [47,48]. Indeed, PL is a plasmatic solution obtained by the cryogenic disruption of the platelets produced from PRP [49].…”

Section: Introductionmentioning

confidence: 99%

Effect of platelet lysate on uterine response of mares susceptible to persistent mating-induced endometritis

et al. 2022

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Treatment With Platelet Lysate Inhibits Proteases of Synovial Fluid in Equines With Osteoarthritis

Cited by 13 publications

References 46 publications

Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models

Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models

Platelet lysate for the treatment of osteoarthritis: a systematic review of preclinical and clinical studies

Effect of platelet lysate on uterine response of mares susceptible to persistent mating-induced endometritis

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