Treatment with roscovitine and butyrolactone I prior to in vitro maturation alters blastocyst production

Abstract

SummaryThis study evaluated the effects of oocyte meiosis inhibitors roscovitine (ROS) and butyrolactone I (BL-I) on in vitro production of bovine embryos. Bovine oocytes were maintained in pre in vitro maturation (pre-IVM) with 25 µM ROS or 100 µM BL-I for 24 h to delay meiosis and for 24 h in in vitro maturation (IVM). Following this treatment, the nuclear maturation index was evaluated. All embryos degenerated following this procedure. In the second set of experiments, oocytes were maintained for 6 or 12 h in pre-IVM with the following three treatments: ROS (25 µM or 12.5 µM), BL-I (100 µM or 50 µM) or a combination of both drugs (6.25 µM ROS and 12.5 µM BL-I). Oocytes were cultivated for 18 or 12 h in IVM. When a meiosis-inducing agent was used during pre-IVM for 24 h, more degenerated oocytes were observed at the end of the IVM period. This effect decreased when the meiotic blocking period was reduced to 6 or 12 h. No significant differences were observed in the blastocyst production rate of oocytes in pre-IVM for 6 h with ROS, BL-I, or ROS + BL-I compared with that of the control group (P > 0.05). However, inhibition of oocytes for 12 h resulted in decreased embryo production compared with that in the controls (P < 0.05). There was no difference in the post-vitrification embryo re-expansion rate between the study groups, showing that the meiotic inhibition for 6 or 12 h did not alter the embryo cryopreservation process.

Coral-Derived Endophytic Fungal Product, Butyrolactone-I, Alleviates Lps Induced Intestinal Epithelial Cell Inflammatory Response Through TLR4/NF-κB and MAPK Signaling Pathways: An in vitro and in vivo Studies

“…Butyrolactone-I (BTL-1) is derived from Aspergillus terreus C23-3, an endophytic fungus isolated from Puko Shore coral (Porites pukoensis) in Xuwen (Guangdong, China) (14). Previous studies have found that butyrolactone-I, as a cyclin CDC-2 kinase and CDK-2 kinase inhibitor (15), can prevent oocytes from decelerating division, inhibit tumor cell proliferation, induce cancer cell apoptosis, and prevent the apoptosis of central and peripheral neurons (16)(17)(18)(19)(20)(21). Presently, researchers have discovered that it has antibacterial and anti-inflammatory activities and inhibits the secretion of NO and TNF-α in macrophages and microglia (22).…”

Impact of Ionomycin on the Gene Expression of In vitro Fertilized Bovine Embryos

“…Sperm straws were thawed for 30 seconds in 37  C water after being frozen. After thawing, a discontinuous Percoll gradient was used (Sigma GE17-0891-01) (45 and 90% (vol/vol) Percoll) (Maziero et al, 2020;Nogueira et al, 2021). Semen was inserted in a 15 mL tube with 5 ml capacitation medium.…”

The Role of Ionomycin in the Improvement of in vitro Fertilization and Increasing the Rate of Bovine Embryo Development

“…Frozen bull semen thawed in 37C water and transferred to a discontinuous percoll gradient (Sigma GE17-0891-01) [45 and 90% (vol/vol) Percoll] (Maziero et al, 2020;Nogueira et al, 2021). The semen was placed in a 15 mL tube containing 5 ml of capacitation medium.…”