Treatment with the combined antimicrobials triggers proteomic changes in P. aeruginosa-C. albicans polyspecies biofilms

Abstract

Pseudomonas aeruginosa is known to coexist and interact with Candida albicans in the polyspecies biofilms. In our previous work, treatment with the combined antimicrobials has been shown to significantly inhibit this polyspecies biofilm; however, the proteomic changes associated with this biofilm inhibition remain not well understood. Thus, the present work was performed to determine the proteome profile of P. aeruginosa-C. albicans polyspecies biofilms following treatment with the combined antimicrobials. The P. aeruginosa-C. albicans polyspecies biofilms were developed in 6-well microplates at 37 °C for 24 h under aerobic and anaerobic conditions. Following the treatment with 40 mg/ml combined antimicrobials (20 mg/ml erythromycin:20 mg/ml nystatin), whole-cell proteomes from the polyspecies biofilms were analyzed by a combination of two-dimensional polyacrylamide gel electrophoresis (2D SDS-PAGE) and mass spectrometry. On the other hand, the differentially expressed proteins were analyzed using STRING database. Results demonstrated that 3 and 9 P. aeruginosa proteins were differentially expressed in treated polyspecies biofilms under aerobic and anaerobic conditions, respectively. Based on the protein interaction network, several biological pathways such as carbohydrate metabolism, protein metabolism, and secondary metabolite metabolism in P. aeruginosa might be affected by the treatment with combined antimicrobials. There were no differentially expressed C. albicans proteins identified herein. It is possible that the inhibition of the polyspecies biofilms by the combined antimicrobial is associated with multiple biological pathways. The combined antimicrobials used in the present study may be useful to manage the diseases caused by P. aeruginosa-C. albicans polyspecies biofilms.

Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens

“…It is known to express multiple essential proteins underlying complex protein interaction networks (Othman & Yahya 2019;Yaacob et al, 2021b). Further investigations of existing natural products, antifungals, and antibiotics are needed to better fight against a wide spectrum of biofilm infections (Johari et al, 2020;Isa et al, 2022;Rashid et al, 2022). In the present study, SMME displayed a non-concentration-dependent antibiofilm effect against all four tested pathogens at different concentrations.…”

Biofilm-associated proteins as potential drug targets

“…These proteins possess various characteristics, including essential metabolic functions within biofilms and the distinction of being non-host homologous (Othman and Yahya, 2019). Although many antibiotics, disinfectants, antifungals and natural products have been demonstrated to possess antibiofilm potential, their effects on expression of the BAPs remain not well investigated (Isa et al, 2022;Kamaruzzaman et al, 2022;Amran et al, 2023;Johari et al, 2023). In this mini review, we explore the multifaceted roles of BAPs in biofilm formation, their implications in infections, and their potential as targets for therapeutic intervention.…”

A Preliminary Study of Antibiofilm Activity of Ethyl Acetate Extracts of Actinomycetes Isolated from Bris Soil, Terengganu against <em>Salmonella typhimurium</em>

“…Chronic infections induced by bacteria organised in a biofilm are difficult to cure because they have a high tolerance to antibiotic concentrations. Various studies have examined the effectiveness of current antibiotics, antifungals, disinfectants, and medicinal herbs on microbial biofilms (Rashid et al 2022, Isa et al 2022, Kamaruzzaman et al 2022, Johari et al 2023, Amran et al 2022.…”