The paper presents the new stability analysis for the collocation Trefftz method (CTM) for biharmonic equations, based on the new effective condition number Cond eff. The Trefftz method is a special spectral method with the particular solutions as admissible functions, and it has been widely used in engineering. Three crack models in Li et al. (Eng. Anal. Boundary Elements 2004; 28:79-96; Trefftz and Collocation Methods. WIT Publishers: Southampton, Boston, 2008) are considered, and the bounds of Cond eff and the traditional condition number Cond are derived, to give the polynomial and the exponential growth rates, respectively. The stability analysis explains well the numerical experiments. Hence, the new Cond eff is more advantageous than Cond. Besides since the bounds of Cond eff and Cond involve the estimation of the minimal singular value min of the discrete matrix F, and since the estimation of min is challenging and difficult, the proof for lower bounds of min in this paper is important and intriguing.where u represents the transverse displacement in the thin plate theory, u = * 2 u/*x 2 +* 2 u/*y 2 , and the solution domain is the rectangle: S = {(x, y)|−1