Trefoil factor 1 suppression of E-CADHERIN enhances prostate carcinoma cell invasiveness and metastasis

Bougen, Nicola M.; Amiry, N.; Yuan, Yan; Kong, Xiangjun; Pandey, Vijay; Vidal, L.; Perry, Jo K.; Zhu, Tao; Lobie, Peter E.

doi:10.1016/j.canlet.2012.12.012

Cited by 21 publications

(14 citation statements)

References 71 publications

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“…Similarly, overexpression of TFF1 stimulates motility or metastasis of human pancreatic stellate or carcinoma cells 32 . Some of these effects may be attributed to TFF1-mediated transcriptional inhibition of E-cadherin 46 . Another possible mechanism involves transactivation of epidermal growth factor receptors 47 …”

Section: Discussionmentioning

confidence: 99%

Gastric Proteins MUC5AC and TFF1 as Potential Diagnostic Markers of Colonic Sessile Serrated Adenomas/Polyps

Khaidakov¹,

Lai

Roudachevski

et al. 2016

Am J Clin Pathol

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Objectives: A subset of colon cancers originates from sessile serrated adenomas/polyps (SSA/Ps). Our goal was to identify markers for SSA/Ps that could aid in distinguishing them from hyperplastic polyps (HPs). Methods: We performed immunostaining for gastric proteins MUC5AC and TFF1 in formalin-fixed, paraffin-embedded (FFPE) samples of HPs (n = 47), SSA/Ps (n = 37), and normal colon (n = 30). Results: Control mucosa expressed only trace amounts of MUC5AC and TFF1. HPs exhibited an 11.3- and 11.4-fold increase in MUC5AC and TFF1 expression confined to the upper segments of the crypts near the luminal surface of the polyps. SSA/Ps displayed on average 1.6-fold (MUC5AC, P < .008) and 1.4-fold (TFF1, P < .03) higher signal intensity for these markers than HPs, with a dramatic coexpression of MUC5AC and TFF1 typically occupying the entire length of the crypt. Immunoperoxidase results were similar to immunofluorescence staining for both MUC5AC and TFF1. Conclusions: Our results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating SSA/Ps from HPs. We also suggest the possibility that crypt morphology may be at least partly due to overproduction of highly viscous gastric mucins and that these proteins may play a role in the serrated pathway to colon carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Gastric Proteins MUC5AC and TFF1 as Potential Diagnostic Markers of Colonic Sessile Serrated Adenomas/Polyps

Khaidakov¹,

Lai

Roudachevski

et al. 2016

Am J Clin Pathol

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“…The induction of Snail leads to transcriptional repression of E-cadherin and the induction of epithelial-mesenchymal transition and cell migration [45], [46]. Interestingly, the forced expression of TFF1 in DU145 prostate cancer cells led to increased expression of Snail as well as decreased expression of E-cadherin [47]. Additionally, MSK1-mediated Snail production in breast cancer cells may lead to increased metastasis of breast cancer cells to bone tissue [44].…”

Section: Discussionmentioning

confidence: 99%

Mitogen- and Stress-Activated Protein Kinases 1 and 2 Are Required for Maximal Trefoil Factor 1 Induction

et al. 2013

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Mitogen- and stress-activated protein kinases 1 and 2 (MSK1 and MSK2), activated downstream of the ERK- and p38-mitogen-activated protein kinase pathways are involved in cell survival, proliferation and differentiation. Following mitogenic or stress stimuli, they mediate the nucleosomal response, which includes phosphorylation of histone H3 at serine 10 (H3S10ph) coupled with transcriptional activation of immediate-early genes. While MSK1 and MSK2 are closely related, their relative roles may vary with cellular context and/or stimuli. However, our knowledge of MSK2 recruitment to immediate-early genes is limited, as research has primarily focused on MSK1. Here, we demonstrate that both MSK1 and MSK2, regulate the phorbol ester 12-O-tetradecanoylphorbol-13-acetate induced expression of the breast cancer marker gene, trefoil factor 1 (TFF1), by phosphorylating H3S10 at its 5′ regulatory regions. The MSK-mediated phosphorylation of H3S10 promotes the recruitment of 14-3-3 isoforms and BRG1, the ATPase subunit of the BAF/PBAF remodeling complex, to the enhancer and upstream promoter elements of TFF1. The recruited chromatin remodeling activity leads to the RNA polymerase II carboxy-terminal domain phosphorylation at the TFF1 promoter, initiating TFF1 expression in MCF-7 breast cancer cells. Moreover, we show that MSK1 or MSK2 is recruited to TFF1 regulatory regions, but as components of different multiprotein complexes.

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“…Most of the human cancers originate from the epithelium because they consist of cells that are characterized by a very high rate of proliferation [15]. The regular epithelium is organized by a series of intercellular junctional complexes usually the adherent junction and desmosomes that connects the cells to one another and to the cellular cytoskeleton of actin filaments and microtubules, such that the epithelium is organized into a framework of single cell layer barrier [16].…”

Section: Resultsmentioning

confidence: 99%

Cell Proliferation and Epithelia Profile in Prostate Cancer and Benign Prostatic Hyperplasia

O.M.¹,

P.T.²,

D.T.³

et al. 2014

TOCJ

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Abstract:Objectives: Prostate cancer (PCa) and Benign Prostatic Hyperplasia (BPH) are the leading cause of urinary tract disorders in males both of which are characterized by rapid proliferation of cells. This study characterizes both Ki-67 and E-Cadherin profiles in BPH and PCa biopsies to determine and compare the extent of aggressiveness of cell proliferation in BPH and PCa.Method: Biopsies were collected from patients clinically diagnosed to be suffering from PCa or BPH and were immunohistochemically labelled using Anti-Ki-67 and Anti-ECAD monoclonal antibodies. The sections were treated with urea followed by incubation in a microwave to activate the antigens. Primary antibody treatment was done in biotinylated goat serum, blocking was done with bovine serum albumin (BSA) and finally secondary antibody staining using Anti-Ki-67 and Anti-ECAD monoclonal antibodies. Results:The expression level of Ki-67 corresponded to the rate of proliferation and size of the tumor cell mass in both PCa and BPH. ECAD expression was highly positive in BPH and less positive in PCa-showing onset of malignancy in PCa. Certain cell clumps (metastases) stained highly positive to Ki-67 and were located at random intervals within the tissue of PCa biopsies, this type of distribution also coincided with ECAD immunonegativity. Conclusion:Ki-67 expression indicates the rate of cell proliferation and the aggressiveness of such tumors, while ECAD shows defective cytoskeleton and extent of malignancy. Correlating ECAD and Ki-67 gives a direct relationship between the rate of cell division, tumor invasion and epithelia structure. This also covers both glandular and fibromuscular epithelium

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Trefoil factor 1 suppression of E-CADHERIN enhances prostate carcinoma cell invasiveness and metastasis

Cited by 21 publications

References 71 publications

Gastric Proteins MUC5AC and TFF1 as Potential Diagnostic Markers of Colonic Sessile Serrated Adenomas/Polyps

Gastric Proteins MUC5AC and TFF1 as Potential Diagnostic Markers of Colonic Sessile Serrated Adenomas/Polyps

Mitogen- and Stress-Activated Protein Kinases 1 and 2 Are Required for Maximal Trefoil Factor 1 Induction

Cell Proliferation and Epithelia Profile in Prostate Cancer and Benign Prostatic Hyperplasia

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