Trehalase immobilization on aminopropyl glass for analytical use

Bachinski, Nilza; Sotero-Martins, Adriana; Paschoalin, Vânia Margaret Flosi; Panek, Anita D.; Paiva, Cláudia N.

doi:10.1002/(sici)1097-0290(19970405)54:1<33::aid-bit4>3.0.co;2-z

Cited by 6 publications

(1 citation statement)

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“…The absorbance was measured at 525 nm to detect the amount of oxidized o-dianisidine. The amount of Gl generated was determined using a Gl standard curve as described by Bachinski et al 14 One unit is defined as the amount of Tre that generates 1 μmol of Gl/min at 25 °C in PBS pH 7.4. The specific activity was calculated to be 300 U/mg.…”

Section: ' Experimental Sectionmentioning

confidence: 99%

Bifunctional Trehalose Anode Incorporating Two Covalently Linked Enzymes Acting in Series

et al. 2011

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A bienzymatic electrode incorporating trehalase (Tre) and glucose oxidase (GOx) covalently bound to the surface of Pt through a functionalized thiol linker (Tre|GOx|Pt) has been designed and assembled and its catalytic properties examined by chemical and electrochemical methods in aqueous phosphate buffer solutions (PBS, pH 7.4). Exposure of Tre|GOx|Pt to PBS containing trehalose (Tr) and subsequent polarization at 0.6 V versus Ag/AgCl yielded after a few minutes well-defined steady-state currents ascribed to the oxidation of hydrogen peroxide generated by the GOx-mediated oxidation of glucose (Gl) produced by the Tre-mediated dissociation of Tr. Plots of the steady-state currents versus [Tr] over the range examined, i.e., 5-25 mM, were found to be linear. Implications of these results toward the development of an implantable biofuel cell as an autonomous energy conversion device for insects are discussed.

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Section: ' Experimental Sectionmentioning

confidence: 99%