Trehalose accumulation enhances tolerance of Saccharomyces cerevisiae to acetic acid

Genetic and biochemical analysis of the reserve carbohydrate metabolism in Candida albicans and Candida rugosa

“…These results are not consistent with the results revealed by early studies that S. cerevisiae accumulated trehalose rapidly under weak acid stress created with sorbic acid (Cheng et al, 1999 [13]). On the other hand, parallel to our results, Yoshiyama et al (2015) [30] reported that acetic acid challenge did not cause the rapid accumulation of trehalose in the exponential phase of cell growth, but they maintained that the accumulation of trehalose helps cells during fermentation under acetic acid stress. The type of organic acid can also have an effect on storage carbohydrate metabolism.…”

Effect of salt and sugar osmotic stress on the viability and morphology of Saccharomyces boulardii

“…On the other hand, the trehalose levels obtained in the culture media with sucrose were similar to those of the control indicating that, unlike S. cerevisiae (Wang et al, 2014), S. boulardii does not use the production of trehalose as a strategy to tolerate osmotic stress. Yoshiyama et al (2015) related the amount of accumulated intracellular trehalose (0.6 mg/g) in S. cerevisiae with an enhanced tolerance to acetic acid. Hounsa et al (1998) found, also in S. cerevisiae under osmotic stress with NaCl and sorbitol, values of trehalose of 2.52 and 2.56 mg/g biomass respectively in the stationary phase.…”

“…Hounsa et al (1998) found, also in S. cerevisiae under osmotic stress with NaCl and sorbitol, values of trehalose of 2.52 and 2.56 mg/g biomass respectively in the stationary phase. Several authors (Yoshiyama et al, 2015;Malgorzata et al, 2015;Hounsa et al, 1998) have indicated that the concentration of intracellular trehalose is directly related to cell survival, however, this is not universal as in the case of S. boulardii under osmotic stress. Glycerol, the main compatible solute in S. cerevisiae, is accumulated intracellularly when the yeast is exposed to osmotic stress (Nevoigt and Stahl, 1997).…”

Cloning, expression and activity optimization of trehalose synthase from Thermus thermophilus HB27

“…In addition, the yields of the exogenous enzymes expressed in Escherichia coli have also been studied and optimized . However, the current studies on TreS have only been limited to gene cloning and enzymatic property characterization (Li et al, 2012;Ryu et al, 2010;Zdziebło and Synowiecki, 2006;Wei et al, 2013;Kushwaha et al, 2011;Yoshiyama et al, 2014) and little is known about the transcription and translation process of the TreS genes. Moreover, there are no reports on the comparison of the expression of TreS genes from different sources and expression vectors with distinct promoters.…”