Trehalose and N-Acetyl Cysteine Alleviate Inflammatory Cytokine Production and Oxidative Stress in LPS-Stimulated Human Peripheral Blood Mononuclear Cells

Bastin, Ali Reza; Nazari-Robati, Mahdieh; Sadeghi, Hossein; Doustimotlagh, Amir Hossein; Sadeghi, Asie

doi:10.1080/08820139.2021.1891095

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…Then, the co-analysis of antioxidant activities and secondary metabolites revealed that some saccharide and phenol compounds played a key role in protecting cells from oxidative damage ( Table 3 , Figure 6 and Figure 7 ). The monosaccharides, oligosaccharides, and polysaccharides from medicinal plants had been widely reported to confer antioxidant activities by reversing ROS levels and restoring antioxidant enzyme activities [ 25 , 26 ]. Polysaccharides from D. officinale attenuated H 2 O 2 -induced oxidative stress in H9c2 cells by increasing SOD activities and inhibiting intracellular ROS [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, beta-D-lactose has been reported as a bioactive constituent in the extract of a standardized herbal cocktail that modulates oxidative stress in mouse models of major depression and post-traumatic stress disorder [ 25 ]. Trehalose has also been reported to alleviate oxidative stress in peripheral blood mononuclear cells stimulated by lipopolysaccharides [ 26 ]. This means that some oligosaccharides were still important for the antioxidant activities of Dendrobium extracts but not only polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

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Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Rao

Zhao

et al. 2023

Metabolites

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The medicinal plant Dendrobium nobile is an important natural antioxidant resource. To reveal the antioxidants of D. nobile, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for metabolic analysis. The H2O2-induced oxidative damage was used in human embryonic kidney 293T (H293T) cells to assess intracellular antioxidant activities. Cells incubated with flower and fruit extracts showed better cell survival, lower levels of reactive oxygen species (ROS), and higher catalase and superoxide dismutase activities than those incubated with root, stem, and leaf extracts (p < 0.01). A total of 13 compounds were newly identified as intracellular antioxidants by association analysis, including coniferin, galactinol, trehalose, beta-D-lactose, trigonelline, nicotinamide-N-oxide, shikimic acid, 5′-deoxy-5′-(methylthio)adenosine, salicylic acid, isorhamnetin-3-O-neohespeidoside, methylhesperidin, 4-hydroxybenzoic acid, and cis-aconitic acid (R2 > 0.8, Log2FC > 1, distribution > 0.1%, and p < 0.01). They showed lower molecular weight and higher polarity, compared to previously identified in vitro antioxidants in D. nobile (p < 0.01). The credibility of HPLC-MS/MS relative quantification was verified by common methods. In conclusion, some saccharides and phenols with low molecular weight and high polarity helped protect H293T cells from oxidative damage by increasing the activities of intracellular antioxidant enzymes and reducing intracellular ROS levels. The results enriched the database of safe and effective intracellular antioxidants in medicinal plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Rao

Zhao

et al. 2023

Metabolites

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show abstract

“…Trehalose, a natural non-toxic disaccharide, has been reported to have promising antioxidant properties in previous studies [38][39][40]. It has been previously demonstrated that trehalose can alleviate oxidative stress in liver cells [41], peripheral blood mononuclear cells [42], the spleen [43], the kidney [21], and so on. However, the specific role of trehalose in alleviating oxidative stress in skeletal muscle and the underlying mechanisms remain poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…Previous research has elucidated the crucial involvement of various signaling pathways in oxidative stress, including the Keap1-Nrf2 pathway [13], the nuclear factor κB (NF-κB) pathway [48], and the mitogen-activated protein kinase (MAPK) pathway [42,49]. Among them, the Keap1-Nrf2 system stands out as a vital and evolutionarily conserved defense mechanism in cells to combat oxidative stress [50,51].…”

Section: Trehalose Increases the Nuclear Localization Of Nrf2 By Incr...mentioning

confidence: 99%

Metabolomic Analysis of Trehalose Alleviating Oxidative Stress in Myoblasts

Zhang,

Qiu,

Zhang

et al. 2023

IJMS

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Trehalose, a naturally occurring non-toxic disaccharide, has attracted considerable attention for its potential in alleviating oxidative stress in skeletal muscle. In this study, our aim was to elucidate the metabolic mechanisms underlying the protective effects of trehalose against hydrogen peroxide (H2O2)-induced oxidative stress in C2C12 myoblasts. Our results show that both trehalose treatment and pretreatment effectively alleviate the H2O2-induced decrease in cell viability, reduce intracellular reactive oxygen species (ROS), and attenuate lipid peroxidation. Furthermore, using NMR-based metabolomics analysis, we observed that trehalose treatment and pretreatment modulate the metabolic profile of myoblasts, specifically regulating oxidant metabolism and amino acid metabolism, contributing to their protective effects against oxidative stress. Importantly, our results reveal that trehalose treatment and pretreatment upregulate the expression levels of P62 and Nrf2 proteins, thereby activating the Nrf2-NQO1 axis and effectively reducing oxidative stress. These significant findings highlight the potential of trehalose supplementation as a promising and effective strategy for alleviating oxidative stress in skeletal muscle and provide valuable insights into its potential therapeutic applications.

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“…Trehalose is a natural disaccharide found in invertebrates, fungi, and many plants. It has value in various potential applications; has anti-inflammatory, antioxidant stress, and anti-cell death effects; and is a non-toxic compound [141,142]. In an AAV α-synuclein rat model of PD, animals that received unilateral AAV1/2 A53T α-synuclein showed a deficit in the use of their paw contralateral to the site of vector injection 3 weeks post-surgery, compared with empty vector controls.…”

Section: Trehalosementioning

confidence: 99%

The Potentiality of Natural Products and Herbal Medicine as Novel Medications for Parkinson’s Disease: A Promising Therapeutic Approach

So,

Lee,

Yang

et al. 2024

IJMS

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As the global population ages, the prevalence of Parkinson’s disease (PD) is steadily on the rise. PD demonstrates chronic and progressive characteristics, and many cases can transition into dementia. This increases societal and economic burdens, emphasizing the need to find effective treatments. Among the widely recognized causes of PD is the abnormal accumulation of proteins, and autophagy dysfunction accelerates this accumulation. The resultant Lewy bodies are also commonly found in Alzheimer’s disease patients, suggesting an increased potential for the onset of dementia. Additionally, the production of free radicals due to mitochondrial dysfunction contributes to neuronal damage and degeneration. The activation of astrocytes and the M1 phenotype of microglia promote damage to dopamine neurons. The drugs currently used for PD only delay the clinical progression and exacerbation of the disease without targeting its root cause, and come with various side effects. Thus, there is a demand for treatments with fewer side effects, with much potential offered by natural products. In this study, we reviewed a total of 14 articles related to herbal medicines and natural products and investigated their relevance to possible PD treatment. The results showed that the reviewed herbal medicines and natural products are effective against lysosomal disorder, mitochondrial dysfunction, and inflammation, key mechanisms underlying PD. Therefore, natural products and herbal medicines can reduce neurotoxicity and might improve both motor and non-motor symptoms associated with PD. Furthermore, these products, with their multi-target effects, enhance bioavailability, inhibit antibiotic resistance, and might additionally eliminate side effects, making them good alternative therapies for PD treatment.

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Trehalose and N-Acetyl Cysteine Alleviate Inflammatory Cytokine Production and Oxidative Stress in LPS-Stimulated Human Peripheral Blood Mononuclear Cells

Cited by 9 publications

References 37 publications

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Metabolomic Analysis of Trehalose Alleviating Oxidative Stress in Myoblasts

The Potentiality of Natural Products and Herbal Medicine as Novel Medications for Parkinson’s Disease: A Promising Therapeutic Approach

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