Trehalose Maintains Vitality of Mouse Epididymal Epithelial Cells and Mediates Gene Transfer

Qu, Bin; Gu, Yihua; Shen, Jian; Qin, Jinzhou; Bao, Jianqiang; Hu, Yuan; Dong, Wuzi

doi:10.1371/journal.pone.0092483

Cited by 8 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems very probable that androgens stimulate some of these factors and then the efficiency of transfection. Recently, authors show that some molecules like trehalose is an important factor to mediate and enhance gene transfer in mouse epididymal epithelial cells (Qu et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

Esponda

2018

Int J Med Surg Sci

View full text Add to dashboard Cite

This paper deals with the efficiency of in vivo gene transfer to the mouse cauda epididymis and its relation to androgens. Previous experiments in the female reproductive tract have indicated that the efficiency of transfection is related to the hormonal stage of the animal, nevertheless no analysis have been done in the male tract. We used in vivo gene transfer to the mouse cauda epididymis employing a gene construction that expresses the Green Fluorescent Protein (GFP). Untreated and Testosterone treated males were employed. Testosterone injections (5 μg/g weight) were done from 2 days before the gene transfer, and treatment continued each day during a total period of 15 days. Fluorescence microscopy observations showed the expression of GFP in the cytoplasm of the principal cells in the epididymal tubules. The application of the QWin Program that measures the percentage of fluorescent areas showed that they are increased in the epididymis of treated males. This increase was particularly observed two days after gene injections (from 32.24 % in untreated animals to 47.62 % in testosterone treated males) and after seven days (from 29.98 % to 43.05 %). The possibility to improve transfection efficiency would increase the knowledge on epididymal physiology and would permit to modify the fertilizing capacity in mammals.

show abstract

Section: Discussionmentioning

confidence: 99%

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

Esponda

2018

Int J Med Surg Sci

View full text Add to dashboard Cite

show abstract

“…Trehalose, a natural disaccharide that includes two glucose molecules, has outstanding water solubility. In addition, it is a safe pharmaceutical excipient [37][38][39]. Because of the good solubility of trehalose in NMP, it could be fully dispersed in the PLGA arrowhead matrix.…”

Section: Design and Fabrication Of Ipmnsmentioning

confidence: 99%

Exploring Trehalose on the Release of Levonorgestrel from Implantable PLGA Microneedles

et al. 2020

View full text Add to dashboard Cite

Hydrophobic drugs wrapped in poly (lactic-co-glycolic acid) (PLGA)-based microneedles (MNs) require a long time to release completely. To obtain the desired duration, it is still necessary to modulate the release of hydrophobic drugs from MNs, while the PLGA composition is unchangeable. In this work, implantable PLGA microneedles (IPMNs) composed of PLGA arrowheads encapsulating levonorgestrel (LNG) and a water-soluble supporting array were designed. We explored trehalose used as a porogen on the release of hydrophobic LNG from PLGA-based MNs. Varying the trehalose content in PLGA arrowheads could induce different rates of drug release. The highest cumulative release of LNG was 76.2 ± 3.9% for IPMNs with 33.3% trehalose during 21 days in vitro, while the cumulative release of LNG was 60.4 ± 3.5% for IPMNs without trehalose. Pharmacokinetic results in rats showed that plasma levels of LNG were sustained for 13 days for IPMNs with 33.3% trehalose and 16 days for IPMNs without trehalose. Furthermore, the PLGA arrowheads with trehalose degraded more rapidly than those without trehalose over 21 days in rats. Consequently, using trehalose as a porogen was a feasible approach to modulate the release of a hydrophobic drug from PLGA-based MNs.

show abstract

“…Culture of primary epididymal epithelial cells (PEECs) was performed as previously described with some minor modifications. 17,18 In brief, 6-to 8-week-old male mice were sacrificed by cervical dislocation, and epididymides were dissected (initial experiments were performed with the use of mT/mG*KspCre mice to evaluate the purity and efficiency of epithelial cell outgrowth). Adventitia and adhering fat were removed, and the epididymis was cut into smaller pieces.…”

Section: Isolation Of Peecsmentioning

confidence: 99%

The Rapamycin-Sensitive Complex of Mammalian Target of Rapamycin Is Essential to Maintain Male Fertility

Schell

Kretz

Liang

et al. 2016

The American Journal of Pathology

View full text Add to dashboard Cite

The mammalian target of rapamycin complex 1 (mTORC1) inhibitor rapamycin and its analogs are being increasingly used in solid-organ transplantation. A commonly reported side effect is male subfertility to infertility, yet the precise mechanisms of mTOR interference with male fertility remain obscure. With the use of a conditional mouse genetic approach we demonstrate that deficiency of mTORC1 in the epithelial derivatives of the Wolffian duct is sufficient to cause male infertility. Analysis of spermatozoa from Raptor fl/fl*KspCre mice revealed an overall decreased motility pattern. Both epididymis and seminal vesicles displayed extensive organ regression with increasing age. Histologic and ultrastructural analyses demonstrated increased amounts of destroyed and absorbed spermatozoa in different segments of the epididymis. Mechanistically, genetic and pharmacologic mTORC1 inhibition was associated with an impaired cellular metabolism and a disturbed protein secretion of epididymal epithelial cells. Collectively, our data highlight the role of mTORC1 to preserve the function of the epididymis, ductus deferens, and the seminal vesicles. We thus reveal unexpected new insights into the frequently observed mTORC1 inhibitor side effect of male infertility in transplant recipients.

show abstract

Trehalose Maintains Vitality of Mouse Epididymal Epithelial Cells and Mediates Gene Transfer

Cited by 8 publications

References 35 publications

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

Foreign Gene Expression in the Mouse Cauda Epididymis is Regulated by Androgens

Exploring Trehalose on the Release of Levonorgestrel from Implantable PLGA Microneedles

The Rapamycin-Sensitive Complex of Mammalian Target of Rapamycin Is Essential to Maintain Male Fertility

Contact Info

Product

Resources

About