Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Abstract

BACKGROUND In the process of biological control, the antagonistic yeasts contend with various stresses that negatively influence yeasts' biocontrol efficiency. In the current study, we investigated the effect of trehalose supplementation on the biocontrol efficiency and oxidative stress tolerance of Sporidiobolus pararoseus Y16. RESULTS S. pararoseus Y16, an antagonistic yeast cultured in trehalose supplemented medium, exhibited better biocontrol efficiency against Penicillium expansum and Aspergillus tubingensis in table grapes. Trehalose‐treated S. pararoseus Y16 cells showed good proliferation efficiency and oxidative stress tolerance than untreated cells. Increased β‐1,3‐glucanase, catalase, superoxide dismutase activity, and low protein carbonylation were observed in trehalose‐amended S. pararoseus Y16 upon H2O2 exposure. The RNA sequencing results indicated that trehalose significantly altered the transcriptome of S. pararoseus Y16. The GO, KEGG, and COG annotations revealed that the differentially regulated genes corresponded to the various biological process of the yeast. CONCLUSION Our findings suggested that trehalose use could enhance the biocontrol efficiency and oxidative stress tolerance of S. pararoseus Y16. Trehalose supplementation altered the transcriptome of S. pararoseus Y16, particularly the genes that correspond to amino acid metabolism, nucleotide metabolism, and protein modification. Thereby the oxidative stress tolerance and biological control efficiency of S. pararoseus Y16 was enhanced by trehalose. © 2021 Society of Chemical Industry.

Exogenous trehalose application promotes survival by alleviating oxidative stress and affecting transcriptome in ethanol-stressed Wickerhamomyces anomalus

“…Therefore, the findings of this study suggest that trehalose directly mediates the signal transduction network or indirectly improves osmolyte accumulation, which triggers antioxidant activity and alleviates ethanol-induced adverse effects. Similar results have been reported in studies on plants subjected to various stressful environments (Kosar et al, 2019) and S. pararoseus subjected to oxidative stress (Dhanasekaran et al, 2021). These results suggest that trehalose mitigates intracellular oxidative stress by enhancing the activity of antioxidant enzymes, which may be universal for the response of cells to various stressful environments.…”

“…Moreover, genetically engineering the yeast trehalose-6-phosphate synthase (TPS1) gene in tomatoes enhanced its tolerance against drought, salt and oxidative stress (Cortina and Culi añez-Maci a, 2005). Additionally, exogenous trehalose application was found to generate oxidative stress tolerance in Sporidiobolus pararoseus (Dhanasekaran et al, 2021), Hanseniaspora uvarum (Apaliya et al, 2018), Candida oleophila (Nie et al, 2019) and Pichia caribbica (Zhao et al, 2013), thereby enhancing their biocontrol efficiency.…”

Metabolomics and Microbiomics Insights into the Differential Surface Fouling of Brown Algae

“…Sporidiobolales was significantly abundant on FE by LEfSe analysis. A member of this taxon is considered an antagonistic yeast with profound biocontrol efficiency against pathogens and may have positive relevance on the surface of FE (Dhanasekaran et al, 2021). Although the genus Mucor is generally known as endophytic or soil-derived, it has been also isolated from red algae (Dewapriya et al, 2014).…”

Silencing of the BtTPS genes by transgenic plant‐mediated RNAi to control Bemisia tabaciMED

“…1 Under adversity and stress, trehalose is usually used as an energy donor or signal molecule to protect cellular active substances and regulate cell osmotic pressure. [2][3][4] With a high concentration and wide distribution in insects, trehalose is particularly well known as the blood sugar of insects. 5,6 In addition, trehalose exhibits important physiological functions in insects.…”

“…Trehalose is a nonreducing disaccharide that is widely distributed in microorganisms, plants, and invertebrates 1 . Under adversity and stress, trehalose is usually used as an energy donor or signal molecule to protect cellular active substances and regulate cell osmotic pressure 2–4 . With a high concentration and wide distribution in insects, trehalose is particularly well known as the blood sugar of insects 5,6 .…”