Trehalose transport occurs via TreB in Listeria monocytogenes and it influences biofilm development and acid resistance

Wu, Jialun; McAuliffe, Olivia; O’Byrne, Conor

doi:10.1016/j.ijfoodmicro.2023.110165

Cited by 14 publications

(6 citation statements)

References 70 publications

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“…In E. coli , this metabolic shift allows the bacteria to withstand the lethal actions of various stressors, including acidic conditions (Zeng et al 2022 ). In L. monocytogenes , sugar metabolism, specifically trehalose transport via TreB, has been observed to affect the response to acid stress (Wu et al 2023 ), and there is evidence to suggest that PTS systems can sense environmental cues to activate signaling cascades (Gilbreth et al 2004 , Mertins et al 2007 , Liu et al 2013 , Liu et al 2017 , Wu et al 2023 ). Inhibition of carbon catabolite repression may affect these metabolic shifts which occur in response to acid stress.…”

Section: Resultsmentioning

confidence: 99%

The role of the universal sugar transport system components PtsI (EI) and PtsH (HPr) in Enterococcus faecium

Hallenbeck,

Chua,

Collins

2024

FEMS Microbes

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Vancomycin-resistant enterococci (VRE) pose a serious threat to public health because of their limited treatment options. Therefore, there is an increasing need to identify novel targets to develop new drugs. Here, we examined the roles of the universal PTS components, PtsI and PtsH, in Enterococcus faecium to determine their roles in carbon metabolism, biofilm formation, stress response, and the ability to compete in the gastrointestinal tract. Clean deletion of ptsHI resulted in a significant reduction in the ability to import and metabolize simple sugars, attenuated growth rate, reduced biofilm formation, and decreased competitive fitness both in vitro and in vivo. However, no significant difference in stress survival was observed when compared with the wild type. These results suggest that targeting universal or specific PTS may provide a novel treatment strategy by reducing the fitness of E. faecium.

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Section: Resultsmentioning

confidence: 99%

The role of the universal sugar transport system components PtsI (EI) and PtsH (HPr) in Enterococcus faecium

Hallenbeck,

Chua,

Collins

2024

FEMS Microbes

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“…Trehalose is present in a large number of organisms and is a typical stress metabolite. It can form a unique protective film under extremely harsh conditions (such as drying, dehydration, and high osmotic pressure), protecting the structure of biomolecules from being destroyed, thereby maintaining the life characteristics of the organism [ 41 , 42 ]. The genome of Pseudomonas baumannii encodes OtsB and OtsA.…”

Section: Discussionmentioning

confidence: 99%

Effect of baicalin on eradicating biofilms of bovine milk derived Acinetobacter lwoffii

Ma,

Mei,

Liu

et al. 2024

BMC Vet Res

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Background Acinetobacter lwoffii (A.lwoffii) is a serious zoonotic pathogen that has been identified as a cause of infections such as meningitis, bacteremia and pneumonia. In recent years, the infection rate and detection rate of A.lwoffii is increasing, especially in the breeding industry. Due to the presence of biofilms, it is difficult to eradicate and has become a potential super drug-resistant bacteria. Therefore, eradication of preformed biofilm is an alternative therapeutic action to control A.lwoffii infection. The present study aimed to clarify that baicalin could eradicate A.lwoffii biofilm in dairy cows, and to explore the mechanism of baicalin eradicating A.lwoffii. Results The results showed that compared to the control group, the 4 MIC of baicalin significantly eradicated the preformed biofilm, and the effect was stable at this concentration, the number of viable bacteria in the biofilm was decreased by 0.67 Log10CFU/mL. The total fluorescence intensity of biofilm bacteria decreased significantly, with a reduction rate of 67.0%. There were 833 differentially expressed genes (367 up-regulated and 466 down-regulated), whose functions mainly focused on oxidative phosphorylation, biofilm regulation system and trehalose synthesis. Molecular docking analysis predicted 11 groups of target proteins that were well combined with baicalin, and the content of trehalose decreased significantly after the biofilm of A.lwoffii was treated with baicalin. Conclusions The present study evaluated the antibiofilm potential of baicalin against A.lwoffii. Baicalin revealed strong antibiofilm potential against A.lwoffii. Baicalin induced biofilm eradication may be related to oxidative phosphorylation and TCSs. Moreover, the decrease of trehalose content may be related to biofilm eradication.

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“…The ability of L. monocytogenes strains to survive at lethal acidic condition was tested as previously described with minor adjustments [61]. To examine the acid resistance at stationary phase, 100 μL stationary phase culture was mixed with 900 μL BHI pH 2.3 or pH 2.4 in 1.5 mL microcentrifuge tubes and incubated at 37°C statically.…”

Section: Acid Survival Experimentsmentioning

confidence: 99%

A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance inListeria monocytogenes

McAuliffe

O’Byrne

2023

Preprint

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Stomach acid provides a significant innate barrier to the entry of the food-borne pathogen Listeria monocytogenes into the human gastrointestinal tract. A key determinant of acid resistance in this bacterium is the conserved glutamate decarboxylase system, GadD2 (encoded by the gadT2D2 operon), which helps to maintain the intracellular pH during exposure to gastric acid. In this study, we identified a premature stop codon in a gene located immediately downstream of the gadT2D2 operon that was highly linked to an acid sensitive phenotype. When this open reading frame was restored through homologous recombination an acid resistant phenotype resulted. Through a series of genetic, transcriptomic and survival experiments we established that this gene, which we designated gadR, encodes a transcriptional regulator of the gadT2D2 operon. GadR belongs to the RofA family of regulators, primarily found in the streptococci, where they are involved in regulating virulence. The data further showed that gadR plays a critical role in the development of acid resistance in response to mild acid exposure, a response that is known as the adaptive acid tolerance response (ATR). A deletion analysis of the gadT2D2 promoter region identified two 18bp palindromic sequences that are required for the GadR-mediated induction of gadT2D2, suggesting that they act as binding sites for GadR. Overall, this study uncovers a new RofA-like regulator of acid resistance in L. monocytogenes that plays a significant role in both growth phase-dependent and ATR-mediated acid resistance and accounts for previously observed strain-to-strain differences in survival at low pH. The findings have important implications for understanding the behavior of L. monocytogenes in acidic environments and identify a potential target for improved control of this important pathogen.

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Trehalose transport occurs via TreB in Listeria monocytogenes and it influences biofilm development and acid resistance

Cited by 14 publications

References 70 publications

The role of the universal sugar transport system components PtsI (EI) and PtsH (HPr) in Enterococcus faecium

The role of the universal sugar transport system components PtsI (EI) and PtsH (HPr) in Enterococcus faecium

Effect of baicalin on eradicating biofilms of bovine milk derived Acinetobacter lwoffii

A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance inListeria monocytogenes

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