TREM-1 expression on neutrophils and monocytes of septic patients: relation to the underlying infection and the implicated pathogen

Poukoulidou, Thekla; Spyridaki, Aikaterini; Mihailidou, Ira; Kopterides, Petros; Pistiki, Aikaterini; Alexiou, Zoi; Chrisofos, Michael; Dimopoulou, Ioanna; Drimoussis, Panagiotis; Giamarellos‐Bourboulis, Evangelos J.; Koutelidakis, Ioannis; Marioli, Androniki; Mega, Anna; Orfanos, Stylianos E.; Θεοδωρακοπούλου, Μαρία; Tsironis, Christos; Maggina, Nina; Polychronopoulos, Vlasis; Tsangaris, Iraklis

doi:10.1186/1471-2334-11-309

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…More recent work has provided evidence for an antiinflammatory-protective role of Ang-2; in a murine septic model pre-treatment with Ang-2 prolonged survival from lethal infection by Pseudomonas aeruginosa [13]. http Recent studies of the Hellenic Sepsis Study Group conducted in large cohorts of patients [14][15][16] provided evidence that early alterations of the innate and adaptive immune status in sepsis are present and associated with the type of underlying infection, underscoring a pivotal role of the type of infection in the personalized nature of sepsis pathogenesis. In this respect Kritselis and coworkers have shown that kinetics of circulating lipopolysaccharide (LPS) differ according to the type of underlying infection and are associated with final outcome [16].…”

Section: Introductionmentioning

confidence: 98%

Angiopoietin-2 associations with the underlying infection and sepsis severity

Lymperopoulou¹,

Velissaris²,

Kotsaki³

et al. 2015

Cytokine

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Section: Introductionmentioning

confidence: 98%

Angiopoietin-2 associations with the underlying infection and sepsis severity

Lymperopoulou¹,

Velissaris²,

Kotsaki³

et al. 2015

Cytokine

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“…Stimulation of that receptor leads to the production of proinflammatory cytokines, mainly TNF-␣ and IL-8 (18); in parallel, the receptor is shed through cleavage from the cell membrane in a soluble form known as sTREM-1 (6). A recent publication of the Hellenic Sepsis Study Group showed that, when sepsis develops in the context of VAP, the expression of TREM-1 on circulating monocytes and on circulating neutrophils is decreased upon the transition from sepsis to severe sepsis/shock and this is a component of sepsis-induced immunoparalysis (13). The increase of the expression of TREM-1 on the cell membrane of monocytes, along with the increased production of sTREM-1 by monocytes, is another finding indicating the reversal of immunoparalysis of circulating monocytes achieved by the clarithromycin treatment.…”

Section: Figmentioning

confidence: 99%

Effect of Clarithromycin in Inflammatory Markers of Patients with Ventilator-Associated Pneumonia and Sepsis Caused by Gram-Negative Bacteria: Results from a Randomized Clinical Study

Spyridaki

Rosario

Antonopoulou

et al. 2012

Antimicrob Agents Chemother

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dOne recent, double-blind, randomized clinical trial with 200 patients showed that clarithromycin administered intravenously for 3 days in patients with ventilator-associated pneumonia (VAP) accelerated the resolution of pneumonia and decreased the risk of death from septic shock and multiple organ dysfunctions (MODS). The present study focused on the effect of clarithromycin on markers of inflammation in these patients. Blood was drawn immediately before the administration of the allocated treatment and on six consecutive days after the start of treatment. The concentrations of circulating markers were measured. Monocytes and neutrophils were isolated for immunophenotyping analysis and for cytokine stimulation. The ratio of serum interleukin-10 (IL-10) to serum tumor necrosis factor alpha (TNF-␣) was decreased in the clarithromycin group compared with the results in the placebo group. Apoptosis of monocytes was significantly increased on day 4 in the clarithromycin group compared with the rate of apoptosis in the placebo group. On the same day, the expression of CD86 was increased and the ratio of soluble CD40 ligand (sCD40L) to CD86 in serum was unchanged. The release of TNF-␣, IL-6, and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) by circulating monocytes after stimulation was greater in the clarithromycin group than in the placebo group. The expression of TREM-1 on monocytes was also increased in the former group. These effects were pronounced in patients with septic shock and MODS. These results suggest that the administration of clarithromycin restored the balance between proinflammatory versus anti-inflammatory mediators in patients with sepsis; this was accompanied by more efficient antigen presentation and increased apoptosis. These effects render new perspectives for the immunotherapy of sepsis.

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“…Written consent was provided from patients or their first-degree relatives for patients unable to consent. None of the enrolled patients participated in a former study published by the Hellenic Sepsis Study Group [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Early changes of the kinetics of monocyte trem-1 reflect final outcome in human sepsis

et al. 2014

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BackgroundTREM-1 (triggering receptor expressed on myeloid cells), a receptor expressed on neutrophils and monocytes, is upregulated in sepsis and seems to tune the inflammatory response. We explored the expression of TREM-1 at the gene level and on cell membranes of monocytes and association with clinical outcome.MethodsPeripheral venous blood was sampled from 75 septic patients (39 patients with sepsis, 25 with severe sepsis and 11 with septic shock) on sepsis days 1, 3 and 7. TREM-1 on monocytes was measured by flow cytometry; gene expression of TREM-1 in circulating mononuclear cells was assessed by real-time PCR. sTREM-1 was measured in serum by an enzyme immunoassay.ResultsAlthough surface TREM-1, sTREM-1 and TREM-1 gene expression did not differ between sepsis, severe sepsis and septic shock on day 1, survivors had greater expression of surface TREM-1 on days 3 and 7 compared to non-survivors. sTREM-1 on non-survivors decreased on day 3 compared to baseline. Patients with increase of monocyte gene expression of TREM-1 from day 1 to day 3 had prolonged survival compared to patients with decrease of gene expression of TREM-1 from day 1 to day 3 (p: 0.031).ConclusionsEarly decrease of gene expression of TREM-1 in monocytes is associated with poor outcome. A reciprocal decrease of the pro-inflammatory surface receptor TREM-1 linked with sepsis-induced immunosuppression may be part of the explanation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12865-014-0063-y) contains supplementary material, which is available to authorized users.

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TREM-1 expression on neutrophils and monocytes of septic patients: relation to the underlying infection and the implicated pathogen

Cited by 18 publications

References 28 publications

Angiopoietin-2 associations with the underlying infection and sepsis severity

Angiopoietin-2 associations with the underlying infection and sepsis severity

Effect of Clarithromycin in Inflammatory Markers of Patients with Ventilator-Associated Pneumonia and Sepsis Caused by Gram-Negative Bacteria: Results from a Randomized Clinical Study

Early changes of the kinetics of monocyte trem-1 reflect final outcome in human sepsis

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