TREM‐2 Receptor Expression Increases with 25(OH)D Vitamin Serum Levels in Patients with Pulmonary Sarcoidosis

Bucová, Mária; Suchánková, Magda; Tibenská, Elena; Tedlová, Eva; Demian, Juraj; Majer, I.; Novosadova, H.; Tedla, Miroslav

doi:10.1155/2015/181986

Cited by 12 publications

(11 citation statements)

References 36 publications

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“…(antibodies used: CD14-PC7, TREM-1-PE, TREM-2-APC, and isotype controls; all from R&D System, Minneapolis, MN, USA). The TREM-1 and TREM-2 analysis that we used in our previous studies [ 30 , 31 ] was performed in compliance with flow cytometry protocol recommended by the manufacturer. TREM-1 and TREM-2 expression is presented as the percentage of TREM-1- and TREM-2-positive cells out of all CD14 + cells.…”

Section: Methodsmentioning

confidence: 99%

TREM-1 and TREM-2 Expression on Blood Monocytes Could Help Predict Survival in High-Grade Glioma Patients

Klučková

Kozák

Szaboova

et al. 2020

Mediators of Inflammation

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Objective. In recent years, the role of the modern inflammatory markers TREM-1 (triggering receptors expressed on myeloid cells) and HMGB1 (high mobility group box 1 protein) in tumorigenesis has begun to be studied. Their role in gliomas is not clear. The aim of our study was to find the role of inflammation in gliomas. Patients and Methods. In 63 adult patients with gliomas and 31 healthy controls, the expressions of TREM-1 and TREM-2 on CD14+ blood cells (method: flow cytometry) and the levels of soluble sTREM-1, HMGB1, IL-6, and IL-10 (Elisa tests) were analyzed. Results. Cox proportional hazard analysis showed that a TREM-1/TREM-2 ratio was associated with reduced overall survival (HR=1.001, P=0.023). Patients with a TREM-1/TREM-2 ratio above 125 survived significantly shorter than patients with a TREM-1/TREM-2 ratio below 125. The percentage of CD14+ TREM-1+ cells was strongly associated with a plasma IL-6/IL-10 ratio (positively) and with IL-10 (negatively). Conversely, we found a higher percentage of CD14+ TREM-2+ monocytes in better surviving patients; these cells could downregulate the exaggerated inflammation and potentiate the phagocytosis in the tumor. The serum levels of HMGB1 negatively correlated with the percentage of CD14+ TREM-1+ cells and with the TREM-1/TREM-2 ratio. The positive correlation between the serum levels of a late proinflammatory cytokine HMGB1 with the percentage of TREM2+ CD14+ monocytes can be explained as an effort for suppression of systemic inflammation by anti-inflammatory acting CD14+ TREM-2+ cells. Conclusion. We showed that the TREM-1/TREM-2 ratio (expression on the surface of blood monocytes) could help predict prognosis in patients with gliomas, especially in high-grade gliomas, and that systemic inflammation has an impact on the patient’s overall survival. This is the first study that showed that TREM expression on monocytes in peripheral blood could help predict prognosis in patients with gliomas.

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Section: Methodsmentioning

confidence: 99%

TREM-1 and TREM-2 Expression on Blood Monocytes Could Help Predict Survival in High-Grade Glioma Patients

Klučková

Kozák

Szaboova

et al. 2020

Mediators of Inflammation

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“…As suggested in the German WES study, several genes involved in calcium ion and vitamin D-related biological activities are probably involved in sarcoidosis predisposition [113]. In sarcoidosis, macrophages activate the conversion of inactive precursor 25(OH)D to active vitamin D metabolite (1-25-(OH)2D3) and hypercalcemia is frequently observed in patients [123,124]. The hormonal form of vitamin D has immuno modulatory and anti-proliferative effects and is able to regulate the mTOR signaling pathway by stimulating expression of DDIT4, which facilitates the assembly and activation of the tuberous sclerosis complex (TSC)1/2 complex for eventual suppression of downstream mTOR activity through Rheb (Ras homolog enriched in the brain) [125].…”

Section: Are We Able To Integrate All the Results From The Genetic Stmentioning

confidence: 97%

Current Insights in Genetics of Sarcoidosis: Functional and Clinical Impacts

Calender

Weichhart

Valeyre

et al. 2020

JCM

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Sarcoidosis is a complex disease that belongs to the vast group of autoinflammatory disorders, but the etiological mechanisms of which are not known. At the crosstalk of environmental, infectious, and genetic factors, sarcoidosis is a multifactorial disease that requires a multidisciplinary approach for which genetic research, in particular, next generation sequencing (NGS) tools, has made it possible to identify new pathways and propose mechanistic hypotheses. Codified treatments for the disease cannot always respond to the most progressive forms and the identification of new genetic and metabolic tracks is a challenge for the future management of the most severe patients. Here, we review the current knowledge regarding the genes identified by both genome wide association studies (GWAS) and whole exome sequencing (WES), as well the connection of these pathways with the current research on sarcoidosis immune-related disorders.

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“…Vitamin D defi ciency is associated with various disorders such as: autoimmune diseases, infections, cardiovascular diseases, asthma bronchiale, chronic pulmonary diseases, severity and mortality of sepsis, and excess mortality in the general population (30,(51)(52)(53)(54)(55).…”

Section: Discussionmentioning

confidence: 99%

Decreased plasma levels of 25(OH)D in multiple sclerosis patients. Correlation with disease severity expressed by EDSS, MSSS, progression index and Herbert´s scale severity grade

Bucová¹,

Ďurmanová²,

Cudrakova³

et al. 2019

BLL

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OBJECTIVES: Multiple sclerosis is a chronic infl ammatory and autoimmune demyelinating disease of the brain and spinal cord. Vitamin D has anti-infl ammatory and anti-Th1, Th17 activities, activates the function of regulatory T cells, shifts the immune response towards Th2, so it might be favorable for downregulation of the disease pathogenesis, and if infl ammation and Th1 and Th17 immunity are hyperactivated. The aim of our study was to highlight the role of vitamin D in multiple sclerosis pathogenesis. METHODS: We investigated 178 patients with multiple sclerosis. Plasma levels of 25(OH)D and HMGB1 were investigated. RESULTS: Despite a regular use of VD by patients, the plasma levels of 25(0H)D were signifi cantly decreased in 57% of them, 14.1% had VD defi ciency (level of 25(OH)D < 20 ng/mL) and more than 6 % of patients had VD severe defi ciency with the plasma level of 25(OH)D < 12 ng/mL. The level of 25(OH)D negatively correlated with the severity of the disease (EDSS, index of progression, duration of the disease) and negative association was found also with Herbert´s six severity grades. HMGB1 levels were higher in patients (p < 0.0001). CONCLUSION: Our result showed that vitamin D defi ciency plays a role in multiple sclerosis pathogenesis. We believe that administration of vitamin D to patients at a suffi cient dose providing a physiological level of vitamin D could have a positive effect on the course of the disease. However, regular monitoring of vitamin D levels is required, which should be at least within 30-75 ng/mL, and even more, but below the toxicity limit

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TREM‐2 Receptor Expression Increases with 25(OH)D Vitamin Serum Levels in Patients with Pulmonary Sarcoidosis

Cited by 12 publications

References 36 publications

TREM-1 and TREM-2 Expression on Blood Monocytes Could Help Predict Survival in High-Grade Glioma Patients

TREM-1 and TREM-2 Expression on Blood Monocytes Could Help Predict Survival in High-Grade Glioma Patients

Current Insights in Genetics of Sarcoidosis: Functional and Clinical Impacts

Decreased plasma levels of 25(OH)D in multiple sclerosis patients. Correlation with disease severity expressed by EDSS, MSSS, progression index and Herbert´s scale severity grade

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