Tremorolytic effect of 5′‐chloro‐5′‐deoxy‐(±)‐<scp>ENBA</scp>, a potent and selective adenosine A1 receptor agonist, evaluated in the harmaline‐induced model in rats

Kosmowska, Barbara; Ossowska, Krystyna; Głowacka, Urszula; Wardas, Jadwiga

doi:10.1111/cns.12692

Cited by 11 publications

(23 citation statements)

References 58 publications

(160 reference statements)

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“…Pramipexole or redistilled water was administered 30 min before harmaline. The doses and timeline of administration of compounds were based on our previous studies [5,6,8]. The behavioral tests using force plate actimeters (FPA) started immediately after harmaline injection and lasted 60 min.…”

Section: Drugsmentioning

confidence: 99%

“…Quantitative in situ hybridization of zif-268 mRNA in rat brain structures was performed according to Kosmowska et al [6]. The 45-mer synthetic oligonucleotide probe used was complementary to the 3-47 bp of the rat zif-268 mRNA gene (NM_012551.2, gi: 148747152).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…The most commonly used animal model to search for substances with anti-ET potential is based on tremor induction by acute harmaline administration. Harmaline produces kinetic/postural tremor of the whole body with the peak of oscillation frequency between 10-12 Hz in rats [3][4][5][6][7][8]. The mechanism of harmaline-induced tremor includes abnormal synchronous activation of the olivo-cerebellar glutamatergic climbing fibers [9] and enhancement of the complex spike discharge of the Purkinje cells (PCs) of the cerebellar cortex [10].…”

Section: Introductionmentioning

confidence: 99%

“…The climbing fibers are also connected directly to the deep cerebellar nuclei (DCN) which send glutamatergic projections to the ventral motor thalamic nuclei [11][12][13], from where the glutamatergic signal continues to be transmitted to the motor cortex [14]. All of the above structures were proven to be involved in the generation and spread of harmaline tremor by an enhanced expression of different neuronal activity markers (c-fos, zif-268) [6,15,16], and in the case of the cerebellum [16,17] and motor thalamus [18], also by an excessive glutamate release.…”

Section: Introductionmentioning

confidence: 99%

“…Since zif-268 expression is generally considered a sensitive neurochemical marker useful in assessing the response of neurons in the examined area of the brain to given stimuli (for Refs. [22,23]), we decided to examine the influence of pramipexole on the level of its mRNA in brain areas which are proven to be affected by harmaline [6], i.e. in the inferior olive (IO), cerebellar cortex, ventroanterior/ventrolateral motor thalamic nuclei (VA/VL) and motor cortex.…”

Section: Introductionmentioning

confidence: 99%

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Pramipexole Reduces zif-268 mRNA Expression in Brain Structures involved in the Generation of Harmaline-Induced Tremor

2020

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Essential tremor is one of the most common neurological disorders, however, it is not sufficiently controlled with currently available pharmacotherapy. Our recent study has shown that pramipexole, a drug efficient in inhibiting parkinsonian tremor, reduced the harmaline-induced tremor in rats, generally accepted to be a model of essential tremor. The aim of the present study was to investigate brain targets for the tremorolytic effect of pramipexole by determination of the early activitydependent gene zif-268 mRNA expression. Tremor in rats was induced by harmaline administered at a dose of 15 mg/kg ip. Pramipexole was administered at a low dose of 0.1 mg/kg sc. Tremor was measured by Force Plate Actimeters where four force transducers located below the corners of the plate tracked the animal's position on a Cartesian plane. The zif-268 mRNA expression was analyzed by in situ hybridization in brain slices. Harmaline induced tremor and increased zif-268 mRNA levels in the inferior olive, cerebellar cortex, ventroanterior/ventrolateral thalamic nuclei and motor cortex. Pramipexole reversed both the harmaline-induced tremor and the increase in zif-268 mRNA expression in the inferior olive, cerebellar cortex and motor cortex. Moreover, the tremor intensity correlated positively with zif-268 mRNA expression in the above structures. The present results seem to suggest that the tremorolytic effect of pramipexole is related to the modulation of the harmaline-increased neuronal activity in the tremor network which includes the inferior olive, cerebellar cortex and motor cortex. Potential mechanisms underlying the above pramipexole action are discussed.

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Section: Drugsmentioning

confidence: 99%

Section: In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pramipexole Reduces zif-268 mRNA Expression in Brain Structures involved in the Generation of Harmaline-Induced Tremor

2020

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Adenosine receptors as promising targets for the management of ocular diseases

et al. 2021

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The ocular drug discovery arena has undergone a significant improvement in the last few years culminating in the FDA approvals of 8 new drugs. However, despite a large number of drugs, generics, and combination products available, it remains an urgent need to find breakthrough strategies and therapies for tackling ocular diseases. Targeting the adenosinergic system may represent an innovative strategy for discovering new ocular therapeutics. This review focused on the recent advance in the field and described the numerous nucleoside and non-nucleoside modulators of the four adenosine receptors (ARs) used as potential tools or clinical drug candidates.

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Increased Purkinje Cell Complex Spike and Deep Cerebellar Nucleus Synchrony as a Potential Basis for Syndromic Essential Tremor. A Review and Synthesis of the Literature

Handforth

Lang

2020

Cerebellum

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Tremorolytic effect of 5′‐chloro‐5′‐deoxy‐(±)‐ENBA, a potent and selective adenosine A1 receptor agonist, evaluated in the harmaline‐induced model in rats

Abstract: This study suggests that adenosine A receptors may be a potential target for the treatment of essential tremor.

Cited by 11 publications

References 58 publications

Pramipexole Reduces zif-268 mRNA Expression in Brain Structures involved in the Generation of Harmaline-Induced Tremor

Pramipexole Reduces zif-268 mRNA Expression in Brain Structures involved in the Generation of Harmaline-Induced Tremor

Adenosine receptors as promising targets for the management of ocular diseases

Increased Purkinje Cell Complex Spike and Deep Cerebellar Nucleus Synchrony as a Potential Basis for Syndromic Essential Tremor. A Review and Synthesis of the Literature

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