2014
DOI: 10.1021/es503406h
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Trenbolone Acetate Metabolite Transport in Rangelands and Irrigated Pasture: Observations and Conceptual Approaches for Agro-Ecosystems

Abstract: To assess the relative ecological risks of trenbolone acetate (TBA) use in agro-ecosystems, we evaluated the spatiotemporal dynamics of TBA metabolite transport during irrigation and rainfall events. Within a pasture, TBA-implanted heifers (40 mg TBA, 8 mg estradiol) were briefly penned (24 h) at high stocking densities (500 animal units (AU)/ha), prior to irrigation. Irrigation runoff concentrations of 17α-trenbolone (17α-TBOH) 0.3 m downslope were 11 ng/L in the wetting front, but quickly decreased to ∼0.5 n… Show more

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Cited by 15 publications
(7 citation statements)
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“…Samples were resuspended in 100 mL N-methyl-N-(trimethylsilyl)-trifluoroacetamine, capped prior to incubation (40 min) at 60 8C, then cooled and analyzed by GC-MS/MS with isotopic dilution analysis. At these microcosm volumes, the method limit of quantification was 14 ng/L, and quality assurance and quality control metrics (near quantitative spike recovery, precision generally within 10%, no false positives detected in blanks) are similar to those we reported in detail for concurrent studies [11,25,26]. Samples that failed either to meet this limit of quantification or to meet a signal-to-noise ratio of 10 were excluded from quantitative analysis.…”
Section: Gas Chromatography-tandem Mass Spectrometry (Gc-ms/ms) Analysismentioning
confidence: 61%
“…Samples were resuspended in 100 mL N-methyl-N-(trimethylsilyl)-trifluoroacetamine, capped prior to incubation (40 min) at 60 8C, then cooled and analyzed by GC-MS/MS with isotopic dilution analysis. At these microcosm volumes, the method limit of quantification was 14 ng/L, and quality assurance and quality control metrics (near quantitative spike recovery, precision generally within 10%, no false positives detected in blanks) are similar to those we reported in detail for concurrent studies [11,25,26]. Samples that failed either to meet this limit of quantification or to meet a signal-to-noise ratio of 10 were excluded from quantitative analysis.…”
Section: Gas Chromatography-tandem Mass Spectrometry (Gc-ms/ms) Analysismentioning
confidence: 61%
“…S2 †). [42][43][44][45][46][47] The tank was placed upslope of the soil trench or VFS system and lled with irrigation water (Browns Valley Irrigation District; pH ¼ 7.6, DOC ¼ 5.7 AE 0.1 mg L À1 ). Prior to experiments, the solution was allowed to equilibrate for 24 hours (Fig.…”
Section: Leachate Generationmentioning
confidence: 99%
“…Another runoff study, which featured a 2‐yr monitoring program, recorded a maximum measured concentration of 17β‐trenbolone of 270 ng/L, whereas 17α‐trenbolone was not detected (Bartelt‐Hunt et al ). Both the Gall et al () and Bartelt‐Hunt et al () studies are in contrast with other simulated cattle‐dosing studies, where concentrations of 17α‐trenbolone were consistently higher than 17β‐trenbolone in wastes originating from dosed animals (Webster et al ; Jones et al , , ).…”
Section: Summary Of Available Literaturementioning
confidence: 88%
“…The principal route of 17b-trenbolone entering the environment is through runoff of wastes (urine and feces) from livestock dosed with trenbolone acetate (Lange et al 2002;Kolok and Sellin 2008;Snow et al 2013;Jones et al 2014bJones et al , 2014c. Hence, aquatic species would be most prone to unintended exposures.…”
Section: Environmental Fate and Exposurementioning
confidence: 99%