Climate change analysis is essential, considering the numerous economic and ecological implications of this critical global environmental issue. This paper analyzes the spatial and temporal trends of mean air temperature in Romania's most important agricultural area, the south and south-eastern region, between 1961 and 2009. In this respect, multiannual (the entire period) and multidecadal ) trends were analyzed using the Mann-Kendall test and Sen's slope method at 23 weather stations, annually, seasonally and for the growing season of the region's main agricultural crops (maize and wheat). Multiannually, the results showed statistically significant temperature increases, on all temporal scales (maximum rate of 0.06°C/year recorded in summer, equivalent to a net temperature rise of 2.82°C), except for the autumn season (cooling without statistical significance). Multidecadally, the 1961-1990 period is marked by a general cooling, especially in autumn (maximum values of -0.07°C/year or over 2°C net cooling). In the 1971-2000 and 1981-2009 periods, a general warming was observed (maximum in summer for both multidecades, when positive rates peaked at 0.09°C/year, or 2.5-3°C net warming), but the warming of the last three decades is the most prominent in terms of spatial average magnitude and trend significance. Upon analysis of the impact of climate warming on agricultural yields (maize) through linear regression, in the 1991-2000 decade, considered as case study, it was found that in 32 % of the total analyzed area there are evident relationships between the two variables (p value \0.05). In this case, a dependency of 33-50 % (40 %, on average) of maize to climate was found, and a sensitivity (loss) ranging between 0.9 and 1.5 t/ha/year (1.2 t/ha/year, on average) for a 1°C temperature rise. At the same time, significant losses (of up to 1.7 t/ha/year) of maize for a 1°C temperature rise were identified in 51 % of the area, but with little p value significance (between 0.05 and 0.1). It is however necessary to analyse the agro-climatic results cautiously, considering that only one decade of climate-agriculture relationship was studied. The results can be useful first and foremost for mitigating the climate change impact on agricultural systems, by prioritizing future adaptation strategies enforced by policy makers.