Trending biocontrol strategies against Cronobacter sakazakii: A recent updated review

Chauhan, Rajni; Singh, Niharika; Pal, Gaurav Kumar; Goel, Gunjan

doi:10.1016/j.foodres.2020.109385

Cited by 25 publications

(20 citation statements)

References 145 publications

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“…Although the bacterium had been classified as Enterobacter sakazakii in 1980 (Farmer et al, 1980), it was later reclassified as a new genus, Cronobacter, containing seven species, namely, C. sakazakii, Cronobacter malonaticus, Cronobacter muytjensii, Cronobacter turicensis, Cronobacter dublinensis, Cronobacter universalis, and Cronobacter condimenti (Iversen et al, 2007(Iversen et al, , 2008Joseph et al, 2012). Although the environmental reservoir of C. sakazakii is not evidently understood yet, reconstituted powdered infant formula (PIF) is reported to be the most associated vehicle for transmission (Chauhan et al, 2020;Parra-Flores et al, 2020). The desiccation tolerance of C. sakazakii ensures its survival in PIF and contributes to the increased exposure of neonates to the organism (Bennour Hennekinne et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain

Qian

Huang

et al. 2022

Front. Microbiol.

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Cronobacter sakazakii is an opportunistic pathogen causing a lethality rate as high as 80% in infants. Desiccation tolerance ensures its survival in powdered infant formula (PIF) and contributes to the increased exposure to neonates, resulting in neonatal meningitis, septicemia, and necrotizing enterocolitis. This study showed that a food-isolated C. sakazakii G4023 strain exhibited a stronger desiccation tolerance than C. sakazakii ATCC 29544 strain. Considering the proven pathogenicity of G4023, it could be a big threat to infants. Transcriptome and proteome were performed to provide new insights into the desiccation adaptation mechanisms of G4023. Integrated analyses of these omics suggested that 331 genes were found regulated at both transcriptional and protein levels (≥2.0- and ≥1.5-fold, respectively). Deletion of chemotaxis system encoded genes cheA and cheW resulted in decreased tolerance in both short- and long-term desiccation. Reduced O-antigen chain length contributed to the biofilm formation and desiccation tolerance in the short term rather than the long term. In addition, biosynthesis of flagella, arginine and its transport system, and Fe/S cluster were also observed regulated in desiccated G4023. A better understanding of desiccation adaptation mechanisms of G4023 could in turn guide the operations during production and preservation of PIF or other food to reduce survival odds of G4023 and lower its exposure to get to infants.

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Section: Introductionmentioning

confidence: 99%

Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain

Qian

Huang

et al. 2022

Front. Microbiol.

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“…Several biological methods have been examined over the past few years to reduce stress tolerance or to control Cronobacter growth in PIF as reviewed by Chauhan et al (2020b). However, in vitro efficacy of a given antimicrobial compound does not always match to its in vivo potential.…”

Section: Introductionmentioning

confidence: 99%

Multivariate analysis of adaptive response to ferulic acid and p ‐coumaric acid after physiological stresses in Cronobacter sakazakii

et al. 2021

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Aim: The present study demonstrated the antimicrobial activity of ferulic acid and p-coumaric acid against unstressed and stressed (cold stressed, starved and desiccated) Cronobacter sakazakii in laboratory media (37°C) and reconstituted powdered infant formulation (PIF) with mild heat treatment (50°C). Methods and results: Five phenolics, namely, quercetin, rutin, caffeic acid, ferulic acid and p-coumaric acid, were tested for antimicrobial activities against five strains of C. sakazakii either unstressed or stressed. Strain specific higher resistance to ferulic acid and p-coumaric acid was observed after stress adaptation in laboratory media. The effect of cross protection was validated using reconstituted PIF as delivery vehicle of selected compounds. Both pcoumaric acid and ferulic acid showed inhibition of C. sakazakii in a dose and time dependent manner as revealed by their viable cell counts. Principal component analysis revealed that the desiccated cells were more sensitive to phenolics in reconstituted PIF. Conclusions: Only ferulic acid and p-coumaric acid showed marked antibacterial activity with minimum inhibitory concentration in the range of 2Á5-5 mg ml À1 for unstressed C. sakazakii cells in tryptone soy broth. The maximum inhibition was achieved with 20 mg ml À1 of both the tested polyphenols in reconstituted PIF. Cold stress and starvation stress did not impart any protection nor increased the susceptibility of C. sakazakii, whereas desiccation resulted in increased susceptibility to phenolic compounds. Significance and Impact of the Study: The results obtained in this study helps in understanding the effect of environmental stresses during processing on susceptibility of C. sakazakii to natural antimicrobial agents. Future transcriptomic studies and functional genetic studies are warranted to understand the strain specific stress responses for the development of better control methods possibly by using these natural antagonists.

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“…On April 12, 2002, the FDA had alerted health care professional about the risk of Enterobacter sakazakii (Cronobacter spp.) infection in hospitalized newborn infants, particularly premature infants or other immuno-compromised infants who fed powdered infant formulas [1][2][3][4][5]. This alert issued by FDA was targeted to concerns for immnuocompromised infants in hospital settings [1,[3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…infection in hospitalized newborn infants, particularly premature infants or other immuno-compromised infants who fed powdered infant formulas [1][2][3][4][5]. This alert issued by FDA was targeted to concerns for immnuocompromised infants in hospital settings [1,[3][4][5]. The conventional methods for isolating and identificating E. sakazakii (Cronobacter spp.)…”

Section: Introductionmentioning

confidence: 99%

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Rates of Recovery of Enterobacter sakazakii (Cronobacter spp.) from Powdered Infant Formula Using Both a Chromogenic Agar and Real-Time PCR: A Preliminary Study

Song¹,

Seo²,

Chon³

2021

J. Dairy Sci. Biotechnol.

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Although the number of incidences of illness caused by ingestion of the bacterial pathogen Enterobacter sakazakii (Cronobacter spp.) has dramatically declined, there remains a need for a robust isolation method to recover this microbe from powdered infant formula (PIF). The current method described in the FDA's Bacteriological Analytical Manual requires multiple steps, and 3-4+ days for complete analysis of PIF isolated E. sakazakii (Cronobacter spp.). We describe a bacteriological method including a one-step enrichment followed by plating on chromogenic agar for presumptive identification of E. sakazakii (Cronobacter spp.). Suspected colonies are confirmed by either biochemical analyses, or a Real-Time PCR-based assay. Using this method, E. sakazakii (Cronobacter spp.) in PIF can be isolated and identified within one day (24 hours).

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Trending biocontrol strategies against Cronobacter sakazakii: A recent updated review

Cited by 25 publications

References 145 publications

Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain

Chemotaxis and Shorter O-Antigen Chain Length Contribute to the Strong Desiccation Tolerance of a Food-Isolated Cronobacter sakazakii Strain

Multivariate analysis of adaptive response to ferulic acid and p ‐coumaric acid after physiological stresses in Cronobacter sakazakii

Rates of Recovery of Enterobacter sakazakii (Cronobacter spp.) from Powdered Infant Formula Using Both a Chromogenic Agar and Real-Time PCR: A Preliminary Study

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