Objectives : Instructional design is the systematic approach to the Analysis, Design, Development, Implementation, and Evaluation of learning materials and activities. We aimed to apply the rapid prototyping to instructional systems design (RPISD) in meridianology laboratory, a subject in which students train acupuncture to develop lesson plan. Methods : The needs of the stakeholders including client, subject matter expert and students were analyzed using the performance needs analysis model. Task analysis was implemented by observation and interview. First prototype was drafted and implemented in meridianology laboratory class once. The second prototype was modified from the first, by usability evaluation of the stakeholders. Results : The client requested an electronically documented manual to improve the quality of acupuncture training. The learner requested an extension of practice time and detailed practice guidelines. The main problems of students' performance were some cases of violation of clean needle technique, the lack of communication between the operator and recipient in direct, and lack of confidence in their own performance. Stakeholders were generally satisfied with the proposed first prototype. Second prototype of lesson plan was produced by modifying some contents. Conclusions : A lesson plan was developed by applying the systematic RPISD model. It is expected that the developed instructional design may contribute to the quality improvement of meridianology laboratory education.