Trends in Analytical Scale Separations

Abstract

Although gas chromatography has been called a mature technology, advances are still being made in the development of faster columns as well as detectors which yield more information on solutes. In liquid chromatography short columns packed with 3-micrometer particles are already popular for fast analyses, while long narrow-bore columns are being developed for high-resolution separations. An increasing range of detection modes is enhancing the problem-solving capabilities of liquid chromatography. Electrophoresis continues to be of central importance to molecular biology. Computer-aided analysis and display of electropherogram patterns is helping researchers to better comprehend the wealth of data from two-dimensional electrophoresis. A new mode of two-dimensional electrophoresis is permitting separation of whole chromosomes containing as many as 3 million base pairs.

Analytical separation of polystyrene nanospheres by capillary electrophoresis

“…lncreased ionic strength generally results in enhanced molecular resolution that is brought about by decreasing the electroosmotic flow in a manner similar to that described above (3). On the other hand, increased ionic strength results in higher currents with accompanying increases in Joule heating that can degrade resolution (15).…”

Nanoliter volume sequential differential concentration gradient detector

“…Several research groups are investigating SAW devices for toxic vapor detection because of their small size and excellent sensitivity (3,(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). These planar microsensors consist of…”

Laser beam deflection sensor as a detector for high-efficiency chromatography

“…Recent advances in HPLC technology, using very efficient small diameter microbore and capillary colums, require development of low volume detectors (6,7). One of the promising detector concepts involves the application of a laser.…”