Trends in DNA forensic analysis

Abstract

The paper gives a historical perspective of forensic DNA analysis and overviews existing technologies implemented in forensic laboratories for DNA profiling. Short tandem repeat analysis, mitochondrial DNA and Y-chromosome analysis are described. The review also focuses on emerging new technologies, which represent an interest for the DNA forensic community. Short tandem repeat analysis, by microelectronic chip device, electrophoretic microdevice and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry are reviewed in this context.

Multiplex PCR, amplicon size and hybridization efficiency on the NanoChip electronic microarray

“…Today, almost all DNA analyses performed in forensic laboratories are based on PCR of short tandem repeats (STRs) [21]. The STRs are highly polymorphic and sufficient discrimination can, in most cases, be acquired by analyzing 10-15 STRs.…”

Recent advances in the applications of CE to forensic sciences (2001–2004)

“…In this process CE, and particularly capillary array electrophoretic instrumentation, and microdevice systems play a crucial role. Recent reviews covering the application of capillary electrophoretic techniques to forensic DNA typing have been published by Carey and Mitnik [88] and by Brettel et al [1].…”

DNA‐based Methods for Detection of Genetic Modifications

“…Comparison of transgene and conventional genomes by using species-specific genome-fragment libraries for reference should enable rapid discrimination between GMO and non-GMO samples. Short DNA repeats were recently successfully analyzed by use of MS techniques for forensic purposes (Carey and Mitnik 2002). Although MS analysis of DNA is currently limited by the molecular size of nucleic acids, these detection methods will become increasingly important as high-throughput applications become common.…”