Trends in the enzymatic inhibition by natural extracts

Ávila-Alvarez, Kassandra T.; Chávez‐González, Mónica L.; Segura-Ceniceros, Elda Patricia; Martínez-Hernández, José L.; Arredondo-Valdés, Roberto; Govea-Salas, Mayela; Reyna, Erika Nava; Ilyiná, Anna; Ramos‐González, Rodolfo

doi:10.1016/b978-0-323-89929-1.00030-5

Cited by 5 publications

(5 citation statements)

References 76 publications

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“…On the contrary, the standard drugs, acarbose and tacrine, showed competitive and noncompetitive inhibition of α-amylase and AChE, respectively, which is in agreement with previous reports [ 32 , 39 ]. It is evident that plant extracts exhibit competitive and noncompetitive inhibition due to the presence of a variety of bioactive compounds [ 59 ]. A decrease in V max and no change in K m are characteristics that differentiate noncompetitive inhibition from competitive (no change in V max and an increase in K m ) and uncompetitive (decrease in both V max and K m ) inhibition [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Ahmad

Alrouji

Alhajlah

et al. 2023

Plants

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This study aims to describe the therapeutic potential of C. nocturnum leaf extracts against diabetes and neurological disorders via the targeting of α-amylase and acetylcholinesterase (AChE) activities, followed by computational molecular docking studies to establish a strong rationale behind the α-amylase and AChE inhibitory potential of C. nocturnum leaves-derived secondary metabolites. In our study, the antioxidant activity of the sequentially extracted C. nocturnum leaves extract was also investigated, in which the methanolic fraction exhibited the strongest antioxidant potential against DPPH (IC50 39.12 ± 0.53 µg/mL) and ABTS (IC50 20.94 ± 0.82 µg/mL) radicals. This extract strongly inhibited the α-amylase (IC50188.77 ± 1.67 µg/mL) and AChE (IC50 239.44 ± 0.93 µg/mL) in a non-competitive and competitive manner, respectively. Furthermore, in silico analysis of compounds identified in the methanolic extract of the leaves of C. nocturnum using GC-MS revealed high-affinity binding of these compounds with the catalytic sites of α-amylase and AChE, with binding energy ranging from −3.10 to −6.23 kcal/mol and from −3.32 to −8.76 kcal/mol, respectively. Conclusively, the antioxidant, antidiabetic, and anti-Alzheimer activity of this extract might be driven by the synergistic effect of these bioactive phytoconstituents.

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Section: Discussionmentioning

confidence: 99%

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Ahmad

Alrouji

Alhajlah

et al. 2023

Plants

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“…Enzymes, recognized for their versatility, emerge as key players in disease management. According to the theory of enzyme inhibition, the reversible inhibition of essential enzymes can contribute significantly to alleviating the symptoms associated with the aforementioned diseases [ 45 ]. For example, acetylcholine (AChE) levels are lower in Alzheimer’s patients than in healthy people.…”

Section: Resultsmentioning

confidence: 99%

Impact of In Vitro Gastrointestinal Digestion on the Phenolic Bioaccessibility and Bioactive Properties of Insect-Containing Beef Burgers

Rocchetti,

Zengin,

Giuberti

et al. 2024

Antioxidants

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Mealworm, migratory locust, and house cricket have recently been recognized by the European Commission as novel foods, thus being suitable in different food applications. In this work, we tested their powders as meat extenders at 5% (w/w) inclusion in beef burgers, considering their ability to vehicle phenolic compounds during simulated in vitro static gastrointestinal digestion (INFOGEST). Insect powders were abundant in different phenolic classes, recording the highest values in locust (LP; 314.69 mg/kg), followed by cricket (CP; 113.3 mg/kg) and mealworm (MWP; 51.9 mg/kg). Following a pan-cooking process, LP burgers were confirmed as the best source of phenolics, with a marked abundance of flavonoids and phenolic acids. Interestingly, the insect powders were found to affect the in vitro gastrointestinal bioaccessibility of phenolic compounds when compared with the CTR burger, likely promoted by the interactions between the phenolic compounds and proteins characterizing the tested insect powders. Among the most discriminant phenolic metabolites at the gastrointestinal level, we found several phenolic acids (mainly hydroxycinnamics), recording the highest content for the digested CP-containing burgers. Finally, stilbenes showed significant correlation values at the intestinal level with both antioxidant and enzymatic activities, while total flavonoids were the most correlated with the inhibition of acetylcholinesterase. Taken together, our preliminary findings demonstrated that insect powders added to beef burgers can promote the bioaccessibility and potential bioavailability of phenolics in the distal tracts of the intestine.

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“…Enzymes, which have a leading role in numerous catalytic reactions, also lead to negative effects on food spoilage or human health with their biocatalytic capacities. 16 Enzyme inhibitors dominate the activities of respective enzymes via interacting with the active site. In this context, enzyme inhibitors are an important part of the clinical drug class.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

Deveci,

Tel-Çayan,

Çayan

et al. 2024

ACS Omega

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Mushroom polysaccharides are important bioactive compounds derived from mushrooms with various beneficial properties. In this study, the chemical characterization and bioactivities of polysaccharide extracts from four different edible mushrooms, Clavariadelphus truncatus Donk, Craterellus tubaeformis (Fr.) Queĺ., Hygrophorus pudorinus (Fr.) Fr., and Macrolepiota procera (Scop.) Singer were studied. Glucose (13.24−56.02%), galactose (14.18−64.05%), mannose (2.18−18.13%), fucose (1.21−5.78%), and arabinose (0.04−5.43%) were identified in all polysaccharide extracts by GC-MS (gas chromatography−mass spectrometry). FT-IR (Fourier transform infrared spectroscopy) confirmed the presence of characteristic carbohydrate patterns. 1 H NMR suggested that all polysaccharide extracts had αand β-Dmannopyranose, D-glucopyranose, D-galactopyranose, α-L-arabinofuranose, and α-L-fucopyranose residues. Approximate molecular weights of polysaccharide extracts were determined by HPLC (high-performance liquid chromatography). The best antioxidant activity was found in M. procera polysaccharide extract in DPPH • (1,1-diphenyl-2-picrylhydrazyl) scavenging (39.03% at 800 μg/ mL), CUPRAC (cupric reducing antioxidant capacity) (A 0.50 : 387.50 μg/mL), and PRAP (phosphomolybdenum reducing antioxidant power) (A 0.50 : 384.08 μg/mL) assays. C. truncatus polysaccharide extract showed the highest antioxidant activity in ABTS •+ scavenging (IC 50 : 734.09 μg/mL), β-carotene-linoleic acid (IC 50 : 472.16 μg/mL), and iron chelating (IC 50 : 180.35 μg/mL) assays. Significant anticancer activity was found in C. truncatus polysaccharide extract on HT-29 (IC 50 : 46.49 μg/mL) and HepG2 (IC 50 : 48.50 μg/mL) cell lines and H. pudorinus polysaccharide extract on the HeLa cell line (IC 50 : 51.64 μg/mL). Also, H. pudorinus polysaccharide extract possessed prominent AChE (acetylcholinesterase) inhibition activity (49.14% at 200 μg/mL).

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Trends in the enzymatic inhibition by natural extracts

Cited by 5 publications

References 76 publications

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Secondary Metabolite Profiling, Antioxidant, Antidiabetic and Neuroprotective Activity of Cestrum nocturnum (Night Scented-Jasmine): Use of In Vitro and In Silico Approach in Determining the Potential Bioactive Compound

Impact of In Vitro Gastrointestinal Digestion on the Phenolic Bioaccessibility and Bioactive Properties of Insect-Containing Beef Burgers

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

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