Trends of respiratory virus detection in point-of-care testing: A review

Seok, Youngung; Mauk, Michael G.; Li, Ruijie; Qian, Cheng

doi:10.1016/j.aca.2023.341283

Cited by 10 publications

(5 citation statements)

References 118 publications

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“…Consequently, a definite diagnosis as early as possible is optimal for reducing the risk of transmission. On-site detection is the future trend for pathogen diagnosis, because it is simple, rapid and user-friendly ( Zhao V. X. T. et al, 2020 ; Xiao et al, 2022 ; Hu et al, 2023 ; Seok et al, 2023 ). It has been widely used in many fields of research.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a time-resolved fluorescence labelled immunochromatographic strip assay for rapid and quantitative detection of bovine herpesvirus 1

Liu,

Zhang,

Cheng

et al. 2024

Front. Microbiol.

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Bovine herpes virus 1 (BoHV-1) causes a wide variety of diseases in wild and domestic cattle. The most widely used method for viral identification is real-time PCR, which can only be performed in laboratories using sophisticated instruments by expert personnel. Herein, we developed an ultrasensitive time-resolved fluorescence lateral flow immunochromatographic strip (ICS) assay for detecting BoHV-1 in bovine samples using a monoclonal antibody against BoHV-1 labelled with fluorescent microspheres, which can be applied in any setting. The intact process from sample collection to final result can be achieved in 15 min. The limit of detection of the assay for BoHV-1 was 102 TCID50/100 μL. The coincidence rate of the ICS method and real-time PCR recommended by the World Organization for Animal Health (WOAH) was 100% for negative, 92.30% for positive, and 95.42% for total, as evaluated by the detection of 131 clinical samples. This detection method was specifically targeted to BoHV-1, not exhibiting cross-reactivity with other bovine pathogens including BoHV-5. We developed an ICS assay equipped with a portable instrument that offers a sensitive and specific platform for the rapid and reliable detection of BoHV-1 in the field. The Point-of-Care test of BoHV-1 is suitable for the screening and surveillance of BoHV-1 in dairy herds.

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Section: Discussionmentioning

confidence: 99%

Development and evaluation of a time-resolved fluorescence labelled immunochromatographic strip assay for rapid and quantitative detection of bovine herpesvirus 1

Liu,

Zhang,

Cheng

et al. 2024

Front. Microbiol.

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“…Multiple clinical specimen types for non-invasive detection include the upper respiratory tract (nasopharyngeal, oropharyngeal, and saliva), lower respiratory tract (deep cough sputum and bronchoalveolar lavage fluid), digestive tract (anal swab), and urinary tract (urine) 77 . A range of laboratory-based non-invasive diagnostic tools are available as follows: rapid point-of-care tests (POCT) (rapid antigen detection, loop-mediated isothermal amplification (LAMP) assays, recombinase polymerase amplification assays, microfluidic chips) 78 , standard analysis (serum immunoassay using antibodies, urine antigen detection, immunochemistry, smear microscopy, blood/sputum cultures, electron microscopy, mass spectrometry assay, and molecular diagnostics) 79 , 80 , and auxiliary detection (whole blood cells counts, procalcitonin, C-reactive protein, D-dimer, T-cell activation-induced marker assays) 81 , 82 . Molecular diagnostics is considered the gold standard for viral diagnosis 83 .…”

Section: Current Circulating Respiratory Pathogens In Chinamentioning

confidence: 99%

Respiratory pathogenic microbial infections: a narrative review

Long,

Zheng,

et al. 2024

Int. J. Med. Sci.

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Respiratory infectious diseases have long been recognised as a substantial global healthcare burden and are one of the leading causes of death worldwide, particularly in vulnerable individuals. In the post COVID-19 era, there has been a surge in the prevalence of influenza virus A and other multiple known viruses causing cold compared with during the same period in the previous three years, which coincided with countries easing COVID-19 restrictions worldwide. This article aims to review community-acquired respiratory illnesses covering a broad spectrum of viruses, bacteria, and atypical microorganisms and focuses on the cluster prevalence of multiple known respiratory pathogens in China, thereby providing effective prevention and control measures.

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“…Analytical chemical methods like centrifugation, microscopy, electrophoresis, immunoassays, and nucleic acid analysis play a crucial role in virus research and disease detection. 15–17…”

Section: Introductionmentioning

confidence: 99%