Trends on Chlamydomonas reinhardtii growth regimes and bioproducts

Abstract

The green microalga Chlamydomonas reinhardtii is a model microorganism for several areas of study. Among the different microalgae species, it presents advantageous characteristics, such as genomes completely sequenced and well‐established techniques for genetic transformation. Despite that, C. reinhardtii production is still not easily commercially viable, especially due to the low biomass yield. So far there are no reports of scientometric study focusing only on C. reinhardtii biomass production process. Considering the need for culture optimization, a scientometric research was conducted to analyze the papers that investigated the growth regimes effects in C. reinhardtii cultivation. The search resulted in 130 papers indexed on Web of Science and Scopus platforms from 1969 to December 2022. The quantitative analysis indicated that the photoautotrophic regime was the most employed in the papers. However, when comparing the three growth regimes, the mixotrophic one led to the highest production of biomass, lipids, and heterologous protein. The production of bioproducts was considered the main objective of most of the papers and, among them, biomass was the most frequently investigated. The highest biomass production reported among the papers was 40 g L−1 in the heterotrophic growth of a transgenic strain. Other culture conditions were also crucial for C. reinhardtii growth, for instance, temperature and cultivation process.

Transcriptome analysis reveals the molecular mechanism of differences in growth between photoautotrophy and heterotrophy in Chlamydomonas reinhardtii

“…The Chlorella protothecoides had been described, in which lipid content was much higher than in photoautotrophic cultures [25]. The previous results indicated that cultivation conditions had signi cant effects on C. reinhardtii biomass productivity and bioproduct accumulation [30]. It demonstrated that the biomass, carbohydrates, and chlorophyll b production were higher in photoautotrophic growth.…”

Transcriptome analysis reveals the molecular mechanism of differences in growth between photoautotrophy and heterotrophy in Chlamydomonas reinhardtii

“…It has been reported that heterotrophic growth of the diatom Nitschia laevis , the dinoflagellate Crypthecodinium cohnii , and the rhodophyte Galdieria sulphuraria accumulated much higher cell densities and biomass productivities than autotrophic growth cell ( De Swaaf et al., 2003 ; Wen and Chen, 2003 ; Schmidt et al., 2005 ; Graverholt and Eriksen, 2007 ). The previous results indicated that cultivation conditions had significant effects on C. reinhardtii biomass productivity and bioproduct accumulation ( Pessoa et al., 2023 ). It demonstrated that the biomass, carbohydrates, and chlorophyll b production were higher in photoautotrophic growth.…”

Increasing lipid production in Chlamydomonas reinhardtii through genetic introduction for the overexpression of glyceraldehyde-3-phosphate dehydrogenase

“…Chlamydomonas reinhardtii, a unicellular biflagellate green alga, has been a focal point of research for several decades. Its high growth rate, capacity for sexual reproduction, and ease of cultivation have enabled C. reinhardtii to emerge as a microalgal model strain in both basic physiological research and applied biotechnology (Masi et al, 2023;Pessoa et al, 2023). Indeed, research on C. reinhardtii has significantly advanced the understanding of algal metabolism and has developed molecular tools and techniques for genetic engineering (Milito et al, 2023;Perozeni and Baier, 2023).…”