Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

Cogoni, Valentina; Morgan-Smith, Alex; Fenno, J. Christopher; Jenkinson, Howard F.; Dymock, David

doi:10.1099/mic.0.055939-0

Cited by 35 publications

(46 citation statements)

References 62 publications

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“…In the present study, treatment of T. denticola with PMSF did not affect coaggregation. Similar results were reported with respect to adherence during biofilm formation by T. denticola [37,38]. Dentilisin-deficient mutants of T. denticola reduce binding to fibrinogen, whereas adherence of T. denticola to fibrinogen is not affected by PMSF treatment [37].…”

Section: Discussionsupporting

confidence: 76%

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Dentilisin involvement in coaggregation between Treponema denticola and Tannerella forsythia

et al. 2014

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Periodontitis arises from a biofilm consisting of gram-negative anaerobic rods and spirochetes. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, termed the Red complex, have been co-isolated with high frequency from chronic periodontitis lesions, and these microorganisms are thought to be major pathogens of the disease. Coaggregation is an important strategy in the colonization of dental plaque biofilm by these bacteria. In the present study, we investigated the coaggregation of T. denticola strains with T. forsythia ATCC 43037 by use of visual grading or spectrophotometry. T. denticola ATCC 35405 coaggregated with T. forsythia, reaching a plateau at approximately 60 min. This coaggregation was inhibited by heat treatment of T. denticola ATCC 35405, but not of T. forsythia. Disaccharides such as sucrose, maltose, and lactose inhibited coaggregation by approximately 50%. The coaggregation reaction varied among T. denticola strains. There was somewhat less coaggregation between T. denticola ATCC 33520 and T. forsythia than between T. denticola ATCC 35405 and T. forsythia, although this difference was not statistically significant; T. denticola ATCC33521 showed a trace level of coaggregation with T. forsythia. The magnitude of coaggregation among the three T. denticola strains was proportional to their dentilisin activities. Inactivation of dentilisin abolished coaggregation activity, but inactivation of the major outer sheath protein did not. Phenylmethylsulfonyl fluoride (PMSF) also did not affect coaggregation. These results indicate that dentilisin is involved indirectly in the coaggregation between T. denticola and T. forsythia, because its proteolytic activity is not required, possibly via ligand maturation.2

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Section: Discussionsupporting

confidence: 76%

“…gingivalis, Prevotella intermedia, and F. nucleatum;, however, PMSF treatment does not affect this adherence [38]. The data from the present study and, as well as those from previous reports, indicate that degradation of the surface protein of T. forsythia by the dentilisin protease is not involved in coaggregation.…”

Section: Discussionsupporting

confidence: 70%

Dentilisin involvement in coaggregation between Treponema denticola and Tannerella forsythia

et al. 2014

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“…1 The study suggests that this bacterial access key could be a drug target for people who are at high risk of developing gum diseases.…”

Section: Study Targets 'Community Gangs'mentioning

confidence: 99%

Very brief advice training

2012

Br Dent J

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“…Mixed communities of Streptococcus and Veillonella species, micromanipulated from dental plaque, have been developed using saliva as a sole source of nutrients [57]. More recently, these model systems have been used to identify new mechanisms of co-operation involving protease interactions between T. denticola and P. gingivalis [58,59] and AI-2 signalling between S. gordonii and C. albicans [42]. [37].…”

Section: Advances In Model Systems For Studying Oral Biofilmsmentioning

confidence: 99%

Recent Advances in Studies of Polymicrobial Interactions in Oral Biofilms

James

2013

Curr Oral Health Rep

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The oral cavity supports a complex and finely balanced consortia of microbial species, many of which co-operate within structured biofilms. These communities develop through multitudinous synergistic and antagonistic inter-species relationships. Changes in the dynamics of oral microbial populations are associated with the transition from healthy teeth and gums to dental caries, gingivitis and periodontitis. Understanding the ecology of oral biofilm communities, and how different species communicate within a given host, will inform new strategies for treatment and prevention of oral diseases. Advances in sequencing technologies have fuelled an increasing trend towards global genomic and proteomic approaches to determine the key factors that initiate oral diseases. Whilst metabolic profiling seeks to identify phenotypic changes of whole microbial communities; transcriptomic studies are exploring their complex interactions with each other and the host. This review discusses the most recent in vitro and in vivo studies of interspecies interactions within polymicrobial oral biofilms.

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Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

Cited by 35 publications

References 62 publications

Dentilisin involvement in coaggregation between Treponema denticola and Tannerella forsythia

Dentilisin involvement in coaggregation between Treponema denticola and Tannerella forsythia

Very brief advice training

Recent Advances in Studies of Polymicrobial Interactions in Oral Biofilms

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