Treponema parvum sp. nov., a small, glucoronic or galacturonic acid-dependent oral spirochaete from lesions of human periodontitis and acute necrotizing ulcerative gingivitis.

Wyss, C.; Dewhirst, Floyd E.; Gmür, Rudolf; Thurnheer, Thomas; Yi, Xue; Schüpbach, Peter; Guggenheim, B; Paster, Bruce J.

doi:10.1099/00207713-51-3-955

Cited by 53 publications

(46 citation statements)

References 31 publications

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“…We did not find any unique region situated in an area of the 16S rRNA where the fluorescent signal could be detected. To our knowledge, T. parvum has not been found in BDD, but has been isolated from human periodontitis (Wyss et al, 2001) and a porcine shoulder ulcer .…”

Section: Discussionmentioning

confidence: 99%

Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Karlsson

Klitgaard

Jensen

2014

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 99%

Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Karlsson

Klitgaard

Jensen

2014

Veterinary Microbiology

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“…Cellular protein profiling Specific growth requirements 16S rRNA sequencing (Wyss et al, 2001) Detected in 26% primary endodontic lesions…”

Section: Metagenomic Detection Of the Oral Microbiomementioning

confidence: 99%

Exploring the oral bacterial flora: current status and future directions

et al. 2010

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Oral Diseases (2010) 16, 136–145 Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches.

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“…As in other natural habitats, it appears that only a fraction of its inhabitants have been recognized, let alone isolated. Recently, methods to isolate and characterize fastidious anaerobes have been improved considerably, as exemplified by the routine isolation of treponemes from the consortia in periodontal lesions (Wyss et al, 2001).Clinically, the two animals appeared indistinguishable. Yet, phase-contrast microscopy revealed a striking difference in the bacterial populations from the two lesion-surface suspensions: both contained high concentrations of bacteria; however, spirochaetes were detected in only one sample.…”

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confidence: 99%

mentioning

confidence: 99%

Guggenheimella bovis gen. nov., sp. nov., isolated from lesions of bovine dermatitis digitalis

Wyss

Dewhirst²,

Paster³

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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Tierarztpraxis, CH-3186 Dü dingen, SwitzerlandDermatitis digitalis is an economically important ulcerative disease of undetermined aetiology affecting the hooves of cattle. Material was examined from two independent cases of this disease in Switzerland. Cultures from the advancing front of both lesions yielded large numbers of closely related short, mesophilic, non-motile, non-spore-forming, anaerobic, proteolytic, Gram-positive rods. The 16S rRNA gene sequences of strains OMZ 913 T and OMZ 915 were identical and indicate Tindallia magadiensis and Eubacterium saphenum as their closest relatives. Phenotypically, the novel isolates are clearly distinguished from related bacteria by protein and antigen patterns, by cellular fatty acids and by API ZYM activities. The diamino acid of the Gram-positive cell wall is ornithine and the G+C content of OMZ 913 T DNA is 44?4 mol%. The phylogenetic distance from recognized taxa in the phylum Firmicutes is sufficient to place these bovine isolates into a novel genus and species, for which the name Guggenheimella bovis gen. nov., sp. nov. is proposed, with OMZ 913 T (=CIP 108087 T =DSM 15657 T ) as the type strain.Dermatitis digitalis (DD), also known as Mortellaro disease or strawberry foot, is an economically important ulcerative disease affecting the bovine foot in an increasing number of countries including Germany, Italy, Japan, the Netherlands, Switzerland, the UK and the USA and may be the dominant cause of lameness in dairy cows (Blowey & Sharp, 1988;Choi et al., 1997;Collighan & Woodward, 1997; Collighan et al., 2000; Demirkan et al., 1998; Luginbühl & Kollbrunner, 2000; Moter et al., 1998;Schrank et al., 1999;Shibahara et al., 2002;Walker et al., 1995). The reported spread between herds by veterinarians, foot trimmers and purchased animals as well as curing of the condition by antibiotic therapy are characteristics of an infectious disease (Laven, 2001). However, despite extensive research, a specific infectious agent has not been identified, and it has been suggested that the disease may be a polymicrobial infection (Döpfer et al., 1997). The range of bacteria cultured from DD lesions includes Campylobacter sputorum (Shibahara et al., 2002), Porphyromonas levii, Fusobacterium necrophorum, Fusobacterium nucleatum, Prevotella oralis, Prevotella denticola, Prevotella bivia, Treponema brennaborense (Schrank et al., 1999) and as-yet unnamed treponemes (Demirkan et al., 1999;Stamm et al., 2002;Walker et al., 1995). By using culture-independent methods, such as immunocytochemistry and 16S rRNA gene sequence analysis, additional organisms were detected in lesions and were related to Bacteroides levii, Borrelia burgdorferi, Mycoplasma hyopharyngis and several Treponema species (Collighan & Woodward, 1997; Demirkan et al., 1998; Moter et al., 1998). Treponemes have been detected deep in the affected tissue by in situ hybridization using fluorescent 16S rRNAtargeted oligonucleotide probes; however, the eubacterial organisms seen at the front of the lesion appeared not to b...

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Treponema parvum sp. nov., a small, glucoronic or galacturonic acid-dependent oral spirochaete from lesions of human periodontitis and acute necrotizing ulcerative gingivitis.

Cited by 53 publications

References 31 publications

Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Exploring the oral bacterial flora: current status and future directions

Guggenheimella bovis gen. nov., sp. nov., isolated from lesions of bovine dermatitis digitalis

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