TRGV and TRDV repertoire distribution and clonality of T cells from umbilical cord blood

Li, Yangqiu; Chen, Shaohua; Yang, Lijian; Li, Bo; Chan, John Yeuk-Hon; Cai, Dongqing

doi:10.1016/j.trim.2008.10.010

Cited by 23 publications

(30 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Malignant T-ALL clones may express alternative TCRαβ or γδ receptors (8); therefore, the TCR Vγ and Vδ repertoires were also analyzed in all samples of the present case. Since skewed Vγ and Vδ subfamily distribution is a common characteristic of the leukemia patients (19), similar results were expected and detected in the present study. In the present case, clonally expanded Vδ3 T-cell clones of the same size as CDR3 were identified in all samples at various time points (Fig.…”

Section: A B C Dsupporting

confidence: 92%

“…Although the expression of all of the TCR Vγ and Vδ family members was not found in each sample (Fig. 3), the expression pattern did not appear to be significantly different compared with the healthy individuals from a previous study (19), particularly when the patient achieved CR. Significantly, oligoclonally expanded T-cells were detected in certain TCR Vβ (Vβ1, Vβ21 and Vβ24) and Vδ (Vδ3 and Vδ6) subfamilies, while the majority of TCR subfamily T-cells displayed a polyclonal pattern.…”

Section: Case Reportmentioning

confidence: 65%

“…To monitor the T-cells in the BM of the present patient during the burden of disease and evaluate the effects of treatment, repertoire-specific PCR primers and the GeneScan technique were used in combination, as previously described (18)(19)(20). The TCR Vβ (Fig.…”

Section: Case Reportmentioning

confidence: 99%

See 2 more Smart Citations

Arsenic induced complete remission in a refractory T-ALL patient with a distinct T-cell clonal evolution without molecular complete remission: A case report

Huang

et al. 2016

Oncology Letters

Self Cite

View full text Add to dashboard Cite

Section: A B C Dsupporting

confidence: 92%

Section: Case Reportmentioning

confidence: 65%

See 1 more Smart Citation

Arsenic induced complete remission in a refractory T-ALL patient with a distinct T-cell clonal evolution without molecular complete remission: A case report

Huang

et al. 2016

Oncology Letters

Self Cite

View full text Add to dashboard Cite

“…Subsequently, PCR was performed as described by Li et al [27]. Primers for amplification of genes were used based on Table 1.…”

Section: Methodsmentioning

confidence: 99%

A Retrospective Analysis of γδ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps

Lee

Chang²,

Huang³

et al. 2017

ORL

View full text Add to dashboard Cite

Background/Aims: To examine whether γδ T cell is expressed in the nasal mucosa of chronic rhinosinusitis (CRS) patients and its potential association with recurrence of nasal polyps. Methods: Thirty-six patients with CRS with nasal polyps (CRSwNP) and 25 patients with CRS without nasal polyps (CRSsNP) were recruited. Twenty-six patients with other nasal diseases served as controls. The CRSwNP group was divided into the eosinophilic CRSwNP and noneosinophilic CRSwNP groups. The expression of γδ T cells was detected by immunohistochemistry. The expression of each subtype of γδ T cells was detected by using qRT-PCR. All patients underwent nasal endoscopy, and postoperative follow-up lasted over 12 months. CRS patients were evaluated by preoperative VAS scores of symptoms and nasal endoscopy Lund-Kennedy scores. Results: The expression of γδ T cells in the CRSwNP groups was stronger than in the CRSsNP and the control group (p < 0.05, p < 0.05). The expression of Vγ1⁺γδ T cells in the eosinophilic CRSwNP group was higher than that in the CRSsNP group and the control group (p < 0.05, p < 0.05). The expression of γδ T cells was associated with high rate of recurrence, tissue eosinophil infiltration, worse symptom score of nasal obstruction, and higher Lund-Kennedy score (all p < 0.05). Conclusions: Increased expression of γδ T cells in CRSwNP may be associated with recurrence of nasal polyps.

show abstract

“…b2M served as an internal reference and the relative expression of each gene was calculated according to the Ct value as follows: relative expression = 2 -DCt ×100% where D Ct =Ct (TCR Vg) -Ct (b2M) (12). distribution of TCR Vg and Vd T cells and the ways in which T cells proliferate.…”

Section: Mrna Expressionsmentioning

confidence: 99%

Effects of Immunotherapy on the Distribution and Clonality of TCR Vγ and Vδ Subfamily T Cells in Allergic Rhinitis Patients

Yang¹,

Li²,

Wu³

et al. 2012

Journal of Medical Biochemistry

Self Cite

View full text Add to dashboard Cite

Summary: The aim of this study was to investigate the changes in the peripheral specific IgE level, distribution of TCR Vg and Vd subfamily T cells and mRNA expressions of TCR Vg I-III following specific immunotherapy (SIT) with house-dust-mite extract in allergic rhinitis (AR) patients. Ten AR patients undergoing SIT with house-dust-mite extract for 1 year were recruited. Quanti tative analysis of immunofluorescence was performed to detect the serum specific IgE (sIgE) level before and after SIT; RT-PCR-genescan analysis was employed to detect the mRNA expressions of TCR Vg (I-III) and Vd (1-8) in the peripheral mononuclear cells followed by analysis of T cell clonality. Real-time quantitative PCR was applied to detect the expressions of TCR Vg I-III genes. Ten healthy volunteers served as controls. For AR patients, SIT treatment could improve the symptoms, but the serum sIgE level was not markedly decreased. Before SIT, the expressions of TCR Vg I-III gene were similar between AR patients and controls (P>0.05) but markedly decreased after SIT in AR patients (P<0.05 in TCR VgI and VgII). The expressions of TCR Vd (1-8) before and after SIT were 5.3±0.82 and 4.9±0.57, respectively, and that in healthy controls was 5.2±1.40. Vd1, 2, 3 and 6 were the most common genes found in these patients. Significant difference in the TCR Vd6 subfamily T cells was found between the two groups. Polyclonal or biclonal proliferation was found in the T cells of patients before SIT and in healthy controls, but oligoclonal proliferation in only 1 subject before SIT. After SIT, the proportion of patients with oligoclonal proliferation of T cells (6/10) was markedly increased (P<0.05). SIT for 1 year could alter the expressions of TCR Vg I-III genes, the Kratak sadr`aj: Cilj ove studije bio je da se istra`e promene u nivou specifi~nog IgE u perifernoj krvi, distribuciji T }elija iz potporodice TCR Vg i Vd i ekspresiji TCR Vg I-III u mRNK posle specifi~ne imunoterapije (SIT) sa ekstraktom grinja iz ku}ne pra{ine kod pacijenata sa alergijskim rinitisom (AR). Uklju~eno je 10 pacijenata sa AR le~enih specifi~nom imunoterapijom sa ekstraktom grinja iz ku}ne pra{ine tokom 1 godine. Obavljena je kvantitativna analiza imunofluorescencijom kako bi se odredio nivo specifi~nog IgE (sIgE) u serumu pre i posle SIT; primenjena je RT-PCRgenescan analiza radi odre|ivanja ekspresije TCR Vg (I-III) i Vd (1-8) u mRNK u perifernim mononuklearnim }elijama posle ~ega je obavljena analiza klonalnosti T }elija. Kvantitativna PCR analiza u realnom vremenu sprovedena je kako bi se odredila ekspresija TCR Vg I-III gena. Deset zdravih dobrovoljaca slu`ilo je kao kontrolna grupa. Kod pacijenata sa AR, tretman SIT olak{ao je simptome, ali nivo sIgE u serumu nije se zna~ajno snizio. Pre SIT, ekspresija TCR Vg I-III gena bila je sli~na kod pacijenata sa AR i kontrolnih subjekata (P>0,05), ali se zna~ajno snizila posle SIT kod pacijenata sa AR (P>0,05 u TCR VgI i VgII). Ekspresija TCR Vd (1-8) pre i posle SIT bila je 5,3±0,82 i 4,9±0,57, a kod zdravih subjekata 5,2±1...

show abstract

TRGV and TRDV repertoire distribution and clonality of T cells from umbilical cord blood

Cited by 23 publications

References 31 publications

Arsenic induced complete remission in a refractory T-ALL patient with a distinct T-cell clonal evolution without molecular complete remission: A case report

Arsenic induced complete remission in a refractory T-ALL patient with a distinct T-cell clonal evolution without molecular complete remission: A case report

A Retrospective Analysis of γδ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps

Effects of Immunotherapy on the Distribution and Clonality of TCR Vγ and Vδ Subfamily T Cells in Allergic Rhinitis Patients

Contact Info

Product

Resources

About

TRGV and TRDV repertoire distribution and clonality of T cells from umbilical cord blood

Cited by 23 publications

References 31 publications

Arsenic induced complete remission in a refractory T-ALL patient with a distinct T-cell clonal evolution without molecular complete remission: A case report

Arsenic induced complete remission in a refractory T-ALL patient with a distinct T-cell clonal evolution without molecular complete remission: A case report

A Retrospective Analysis of &#x03B3;δ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps

Effects of Immunotherapy on the Distribution and Clonality of TCR Vγ and Vδ Subfamily T Cells in Allergic Rhinitis Patients

Contact Info

Product

Resources

About

A Retrospective Analysis of γδ T Cell Expression in Chronic Rhinosinusitis and Its Association with Recurrence of Nasal Polyps