Triacylglycerol is produced from starch and polar lipids in the green alga Dunaliella tertiolecta

Pick, Uri; Avidan, Omri

doi:10.1093/jxb/erx280

Cited by 38 publications

(42 citation statements)

References 55 publications

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“…However, the higher level of intracellular ROS induced by EL and N-starvation limited the precursors of polysaccharide and enhanced lipid accumulation. The finding that C. reinhardtii converted starch into lipid was also consistent with reported researches [ 94 – 96 ], which might suggest that starch served as the major carbon source for lipid production. Consequently, the carbon partitioning was first oriented to starch as the stored substance in response to N-starvation.…”

Section: Approaches For Enhancing Biomass and Product Accumulation Masupporting

confidence: 92%

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion

Sun

Zhao

Mao

et al. 2018

Biotechnol Biofuels

115

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Microalgae are capable of producing sustainable bioproducts and biofuels by using carbon dioxide or other carbon substances in various cultivation modes. It is of great significance to exploit microalgae for the economical viability of biofuels and the revenues from high-value bioproducts. However, the industrial performance of microalgae is still challenged with potential conflict between cost of microalgae cultivation and revenues from them, which is mainly ascribed to the lack of comprehensive understanding of carbon metabolism and energy conversion. In this review, we provide an overview of the recent advances in carbon and energy fluxes of light-dependent reaction, Calvin–Benson–Bassham cycle, tricarboxylic acid cycle, glycolysis pathway and processes of product biosynthesis in microalgae, with focus on the increased photosynthetic and carbon efficiencies. Recent strategies for the enhanced production of bioproducts and biofuels from microalgae are discussed in detail. Approaches to alter microbial physiology by controlling light, nutrient and other environmental conditions have the advantages of increasing biomass concentration and product yield through the efficient carbon conversion. Engineering strategies by regulating carbon partitioning and energy route are capable of improving the efficiencies of photosynthesis and carbon conversion, which consequently realize high-value biomass. The coordination of carbon and energy fluxes is emerging as the potential strategy to increase efficiency of carbon fixation and product biosynthesis. To achieve more desirable high-value products, coordination of multi-stage cultivation with engineering and stress-based strategies occupies significant positions in a long term.

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Section: Approaches For Enhancing Biomass and Product Accumulation Masupporting

confidence: 92%

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion

Sun

Zhao

Mao

et al. 2018

Biotechnol Biofuels

115

View full text Add to dashboard Cite

show abstract

“…Under stress conditions such as nitrogen (N) deprivation, microalgae synthesized large amounts of TAG (Hu et al ; Slocombe et al ). Pick and Avidan found that TAG accumulated during N deprivation in D. tertiolecta , and around two‐thirds of the carbon in total TAG originated from starch, one‐third was made de novo by CO 2 assimilation (Pick and Avidan ). In this study, the N in culture medium was measured on the seventh day, and no N starvation was observed (Table S3), indicating that the accumulation of neutral lipid was induced by high light instead of N deficiency.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)

Huang

et al. 2019

Phycological Research

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SUMMARY The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina.

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“…It seems that the neutral lipids in D. tertiolecta are mainly composed of TAGs. Supportively, (Rabbani, Beyer, Von Lintig, Hugueney, & Kleinig, 1998) reported that TAGs are the main component of neutral lipid bodies in Dunaliellabardawil.More recently, (Pick & Avidan, 2017) reported that polar lipids and starch made by photosynthetic carbon assimilation at the early stages of N deprivation might be utilized for the synthesis of TAG in D. tertiolecta.…”

Section: Dissection Of Triacylglycerol Productionmentioning

confidence: 99%

Untitled

Çakmak¹

2019

Turk. J. Fish. Aquat. Sci.

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Distinct impacts of ammonium nutrition for simultaneous production of triacylglycerol, lutein, and β-carotene were evaluated in Dunaliella tertiolecta. A physiologically realistic growth system was employed to compare metabolite production and growth characteristics of nitrogen deprived, ammonium-and nitrate supplied D. tertiolecta. The nitrogen dependent changes in D. tertiolecta were reflected as a dramatic decrease in growth related to decreased photosynthetic activity, reduced pigmentation and increased triacylglycerol production as observed in nitrogen deprived microalgae. On the other hand, ammonium nutrition caused a time-dependent increase of neutral lipids, triacylglycerols, chlorophylls and carotenoids, lutein and β -carotene in particular. Finally, ammonium nutrition improved FAME profile of D. tertiolecta for biodiesel production via an exclusive impact on increased saturation.

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Triacylglycerol is produced from starch and polar lipids in the green alga Dunaliella tertiolecta

Abstract: The major carbon source for triacylglycerol (TAG) biosynthesis in Dunaliella is starch, whose production precedes that of TAG under nitrogen deprivation.

Cited by 38 publications

References 55 publications

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion

Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)

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