Triamcinolone acetonide-loaded PLA/PEG-PDL microparticles for effective intra-articular delivery: synthesis, optimization, in vitro and in vivo evaluation

Abou-ElNour, May; Ishak, Rania A.H.; Tiboni, Mattia; Bonacucina, Giulia; Cespi, Marco; Casettari, Luca; Soliman, Mahmoud E.; Geneidi, Ahmed S.

doi:10.1016/j.jconrel.2019.07.030

Cited by 37 publications

(42 citation statements)

References 67 publications

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“…Rheumatoid arthritis (RA) is a chronic and progressive autoimmune disease with proliferative and invasive synovitis, resulting in joint destruction and cartilage and bone erosion . Moreover, RA has often been reported to be associated with periarticular or generalized osteoporosis, decreased muscle function, and low muscle mass.…”

Section: Introductionmentioning

confidence: 99%

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

Huang¹,

Guo

Chitti³

et al. 2019

BioFactors

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Rheumatoid arthritis (RA) is a chronic and accelerated autoimmune illness with proliferative and damaging synovitis, resulting in joint death and cartilage and bone erosion. This study focused on the potential therapeutic effect of wogonin on complete Freund's adjuvant (CFA) induced RA in rats and the underlying mechanisms. Arthritis was experimentally caused in rats by subcutaneously injecting 0.1 mL of CFA into the subplantar area of the left hind paw under moderate anesthesia on day zero. The regular oral doses of indomethacin/wogonin began on day zero and proceeded after injection to day 35. Wogonin reduced arthritic score considerably, enhanced body weight, and reduced paw thickness. Wogonin also boosted red blood cell considerably along with hemoglobin and reduced white blood cell count and erythrocyte sedimentation rate. Wogonin substantially improved an altered level of oxidative stress markers, antioxidant proteins, and inflammatory cytokines in a dose‐dependent way. Wogonin inhibited p38 phosphorylation triggered by CFA and p65 nuclear translocation.

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Section: Introductionmentioning

confidence: 99%

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

Huang¹,

Guo

Chitti³

et al. 2019

BioFactors

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“…The triamcinolone acetonide spectrum showed four characteristic peaks in the range of 1708-1600 cm −1 , indicating its C=C and C=O stretching vibrations. 36,38 The physical mixture also had the same characteristic peaks in the same wavelength range. However, these peaks were absent in the different NPs, which instead showed a major peak at 1740 cm −1 .…”

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confidence: 84%

“…However, the peak disappeared in the DSC spectra of all drug-loaded nanoparticles, indicating the absence of crystalline drug in the NPs or drug solvation in the amorphous carrier. 36 We surmised therefore that triamcinolone acetonide existed in an amorphous or disorderly crystalline phase in dispersion or a solid solution state in the polymeric NPs.…”

mentioning

confidence: 99%

<p>PLGA Nanoparticle Platform for Trans-Ocular Barrier to Enhance Drug Delivery: A Comparative Study Based on the Application of Oligosaccharides in the Outer Membrane of Carriers</p>

Jiang¹,

Jia²,

Qiu³

et al. 2020

IJN

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The trans-ocular barrier is a key factor limiting the therapeutic efficacy of triamcinolone acetonide. We developed a poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) surface modified respectively with 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD), chitosan oligosaccharide and trehalose. Determination of the drug/nanoparticles interactions, characterization of the nanoparticles, in vivo ocular compatibility tests, comparisons of their corneal permeability and their pharmacokinetics in aqueous humor were carried out. Methods: All PLGA NPs were prepared by the single emulsion and evaporation method and the drug-nanoparticle interaction was studied. The physiochemical features and in vitro corneal permeability of NPs were characterized while the aqueous humor pharmacokinetics was performed to evaluate in vivo corneal permeability of NPs. Ocular compatibility of NPs was investigated through Draize and histopathological test. Results: The PLGA NPs with lactide/glycolide ratio of 50:50 and small particle size (molecular weight 10 kDa) achieved optimal drug release and corneal permeability. Surface modification with different oligosaccharides resulted in uniform particle sizes and similar drug-nanoparticle interactions, although 2-HP-β-CD/PLGA NPs showed the highest entrapment efficiency. In vitro evaluation and aqueous humor pharmacokinetics further revealed that 2-HP-β-CD/PLGA NPs had greater trans-ocular permeation and retention compared to chitosan oligosaccharide/PLGA and trehalose/PLGA NPs. No ocular irritation in vivo was detected after applying modified/unmodified PLGA NPs to rabbit's eyes. Conclusion: 2-HP-β-CD/PLGA NPs are a promising nanoplatform for localized ocular drug delivery through topical administration.

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“…As a contrary approach, some biomaterial-based approaches have been designed with non-spherical sizes or enveloped into protective coatings (i.e., PEG or erythrocyte membranes), to avoid or delay macrophage recognition [94,95]. This strategy has been used in the context of intra-articular delivery to achieve a long-lasting release of the drug from the biomaterial, which is not rapidly internalized by macrophages [20,21]. Finally, some nanotechnology-based approaches have been designed to release the drug extracellularly, not inside macrophages, or even using selected ligands to target other cellular components of the joint (i.e., chondrocytes, synoviocytes, fibroblasts), but always aiming to achieve an anti-inflammatory effect.…”

Section: Nanomaterial-based Approaches For Macrophage Manipulation In Oamentioning

confidence: 99%

“…In the last decade, numerous types of nanocarriers have been engineered, such as nanoparticles, liposomes, or hybrid nanosystems to improve the delivery of drugs toward macrophages in the diseased joints ( Figure 1). Interestingly, also some nanostructures have been designed to prevent macrophage uptake to slow down the drug consumption and prolong the therapeutic effect [20,21]. In this manuscript, we review the nano-based drug delivery strategies aimed to manipulate the immune system in the context of OA, with a particular focus on those designed to specifically target and reprogram macrophages.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis

et al. 2020

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Osteoarthritis (OA) is the most common joint pathology causing severe pain and disability. Macrophages play a central role in the pathogenesis of OA. In the joint microenvironment, macrophages with an M1-like pro-inflammatory phenotype induce chronic inflammation and joint destruction, and they have been correlated with the development and progression of the disease, while the M2-like anti-inflammatory macrophages support the recovery of the disease, promoting tissue repair and the resolution of inflammation. Nowadays, the treatment of OA in the clinic relies on systemic and/or intra-articular administration of anti-inflammatory and pain relief drugs, as well as surgical interventions for the severe cases (i.e., meniscectomy). The disadvantages of the pharmacological therapy are related to the chronic nature of the disease, requiring prolonged treatments, and to the particular location of the pathology in joint tissues, which are separated anatomical compartments with difficult access for the drugs. To overcome these challenges, nanotechnological approaches have been investigated to improve the delivery of drugs toward macrophages into the diseased joint. This strategy may offer advantages by reducing off-target toxicities and improving long-term therapeutic efficacy. In this review, we describe the nanomaterial-based approaches designed so far to directly or indirectly manipulate macrophages for the treatment of osteoarthritis.

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Triamcinolone acetonide-loaded PLA/PEG-PDL microparticles for effective intra-articular delivery: synthesis, optimization, in vitro and in vivo evaluation

Cited by 37 publications

References 67 publications

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

Wogonin ameliorate complete Freund's adjuvant induced rheumatoid arthritis via targeting NF‐κB/MAPK signaling pathway

<p>PLGA Nanoparticle Platform for Trans-Ocular Barrier to Enhance Drug Delivery: A Comparative Study Based on the Application of Oligosaccharides in the Outer Membrane of Carriers</p>

Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis

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