Triamcinolone acetonide suppresses interleukin-1 beta-mediated increase in vascular endothelial growth factor expression in cultured rat Müller cells

Itakura, Hirotaka; Akiyama, Hideo; Hagimura, Norikazu; Doi, Hiroshi; Tanaka, Toru; Kishi, Shoji; Kurabayashi, Masahiko

doi:10.1007/s00417-005-0052-1

Cited by 24 publications

(24 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ruboxistaurin blocks protein kinase-ß which is an intracellular mediator involved in VEGFinduced increase in vascular permeability [88]. The antiinflammatory corticosteroid, triamcinolone acetonide, inhibits vascular leakage [89,90], reduces the vitreal level of VEGF [91], the secretion of VEGF by retinal cells [92][93][94], and the cellular effects of VEGF, e.g., the VEGFevoked secretion of matrix metalloproteinases (own unpublished results). However, it is not known whether the edema-resolving therapies used clinically stimulate the fluid absorption from the retinal tissue concomitantly.…”

Section: Stimulation Of Fluid Absorptionmentioning

confidence: 91%

Müller cells as players in retinal degeneration and edema

Reichenbach

Wurm

Pannicke

et al. 2007

Graefe's Arch Clin Exp Ophthalmol

255

177

View full text Add to dashboard Cite

The data suggest that a disturbed fluid transport through Müller cells is (in addition to vascular leakage) a pathogenic factor contributing to the development of retinal edema. Pharmacological re-activation of the retinal water clearance by Müller cells may represent an approach to the development of new edema-resolving drugs. Triamcinolone acetonide, which is clinically used to resolve edema, prevents osmotic swelling of Müller cells as it induces the release of endogenous adenosine and subsequent A1 receptor activation which results in the opening of ion channels. Apparently, triamcinolone resolves edema by both inhibition of vascular leakage and stimulation of retinal fluid clearance by Müller cells.

show abstract

Section: Stimulation Of Fluid Absorptionmentioning

confidence: 91%

Müller cells as players in retinal degeneration and edema

Reichenbach

Wurm

Pannicke

et al. 2007

Graefe's Arch Clin Exp Ophthalmol

255

177

View full text Add to dashboard Cite

show abstract

“…The study reported by Yego EC et al revealed a novel mechanism for high glucose-induced GAPDH nuclear accumulation in Müller cells through production and autocrine stimulation by IL-1β [28]. Itakura H et al showed the results in their study that stimulation of Müller cells by IL-1β induced VEGF mRNA expression [29]. These findings suggest that IL-1ß may play an important role in the pathogenesis of DR by compromising functions of Müller cells.…”

Section: Introductionmentioning

confidence: 86%

Pigment epithelium derived factor as an anti-inflammatory factor against decrease of glutamine synthetase expression in retinal Müller cells under high glucose conditions

Shen

Zhong

Xie

et al. 2010

Graefes Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

show abstract

“…44,45 Corticosteroids are an attractive option due to their broad range of actions including anti-inflammatory and antiangiogenic effects. [46][47][48][49] Corticosteroid-loaded liposomes have been reported for several decades, but drug loading efficacy and release rates have presented significant challenges. The passive loading of hydrophilic corticosteroids in liposomes initially resulted in very low encapsulation efficiencies (<5%).…”

Section: Discussionmentioning

confidence: 99%

Endothelial Protein C–Targeting Liposomes Show Enhanced Uptake and Improved Therapeutic Efficacy in Human Retinal Endothelial Cells

Arta

Eriksen

Melander

et al. 2018

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. To determine whether human retinal endothelial cells (HRECs) express the endothelial cell protein C receptor (EPCR) and to realize its potential as a targeting moiety by developing novel single and dual corticosteroid-loaded functionalized liposomes that exhibit both enhanced uptake by HRECs and superior biologic activity compared to nontargeting liposomes and free drug.METHODS. EPCR expression of HRECs was investigated through flow cytometry and Western blot assays. EPCR-targeting liposomes were developed by functionalizing EPCR-specific antibodies onto liposomes, and the uptake of liposomes was assessed with flow cytometry and confocal laser scanning microscopy. The therapeutic potential of EPCR-targeting liposomes was determined by loading them with prednisolone either through bilayer insertion and/or by remote loading into the aqueous core. The carrier efficacy was assessed in two ways through its ability to inhibit secretion of interleukins in cells stimulated with high glucose and angiogenesis in vitro by using an endothelial cell tube formation assay. RESULTS.HRECs express EPCR at a similar level in both human aortic and umbilic vein endothelial cells. The EPCR-targeting liposomes displayed at least a 3-fold higher uptake compared to nontargeting liposomes. This enhanced uptake was translated into superior antiinflammatory efficacy, as the corticosteroid-loaded EPCR-targeting liposomes significantly reduced the secretion of IL-8 and IL-6 and inhibited the development of cell tube formations in contrast to nontargeting liposomes. CONCLUSIONS.We show that HRECs express EPCR and this receptor could be a promising nanomedicine target in ocular diseases where the endothelial barrier of the retina is compromised.

show abstract

Triamcinolone acetonide suppresses interleukin-1 beta-mediated increase in vascular endothelial growth factor expression in cultured rat Müller cells

Abstract: TA suppressed VEGF expression induced by IL-1b in Müller cells at the transcriptional level. Our data sustained the clinical effect of TA for diabetic macular edema and suggested an important role of TA for the suppression of the VEGF gene expression in ocular tissues.

Cited by 24 publications

References 43 publications

Müller cells as players in retinal degeneration and edema

Müller cells as players in retinal degeneration and edema

Pigment epithelium derived factor as an anti-inflammatory factor against decrease of glutamine synthetase expression in retinal Müller cells under high glucose conditions

Endothelial Protein C–Targeting Liposomes Show Enhanced Uptake and Improved Therapeutic Efficacy in Human Retinal Endothelial Cells

Contact Info

Product

Resources

About