Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste ‘extract’ for enhanced and concomitant lipid and β-carotene production

Abstract

BackgroundVegetable ‘mandi’ (road-side vegetable market) waste was converted to a suitable fermentation medium for cultivation of oleaginous yeast Rhodosporidium toruloides by steaming under pressure. This cultivation medium derived from waste was found to be a comparatively better source of nutrients than standard culture media because it provided more than one type of usable carbon source(s) to yeast.ResultsHPLC results showed that the extract contained glucose, xylose and glycerol along with other carbon sources, allowing triauxic growth pattern with preferably usage of glucose, xylose and glycerol resulting in enhanced growth, lipid and carotenoid production. Presence of saturated and unsaturated fatty acid methyl esters (FAMEs) (C14-20) in the lipid profile showed that the lipid may be transesterified for biodiesel production.ConclusionUpscaling these experiments to fermenter scale for the production of lipids and biodiesel and other industrially useful products would lead to waste management along with the production of value added commodities. The technique is thus environment friendly and gives good return upon investment.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1026-4) contains supplementary material, which is available to authorized users.

Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products inR. toruloidesto include non-ribosomal peptides

“…S7 †), possibly due to remobilization of storage lipids accumulated during earlier stages of the cultivation as a result of the onset carbon depletion at late stages of the cultivation. 55,56 This observation further supports the hypothesis, that higher C/N ratios favor storage lipid accumulation over completion of the TCA cycle and underlines the importance of a customized carbon-tonitrogen ratio for efficient production of different products. Further experiments are needed to fully understand the regulatory mechanisms underlying the balance between lipid biogenesis and TCA cycle progression.…”

The Oleaginous Red YeastRhodotorula/Rhodosporidium: A Factory for Industrial Bioproducts

“…This lipid production can occur on a variety of different carbon sources, e.g. sugarcane juice, crude glycerol, lignocellulosic hydrolysates, vegetable market waste, and Jerusalem artichoke plants [45][46][47][48][49].…”

Camelina sativa meal hydrolysate as sustainable biomass for the production of carotenoids by Rhodosporidium toruloides

“…R. toruloides can use different sugars, such as glucose, cellobiose, sucrose, mannose, xylose, arabinose, and galacturonic acid, as main carbon sources [20]. In addition, R. toruloides converts complex substrates, such as carob pulp syrup, sugarcane bagasse, corn stover, and food wastes, into lipids and/or carotenoids [21][22][23][24]. Therefore, this yeast is a good candidate for the development of second-generation biorefineries.…”