The main focus of this work is to investigate the impact of varying milling times (2 to 18 h) on the structural and mechanical properties of the developed Ti-Nb-Mo alloy. The morphology, phase composition, microstructure, and mechanical behavior of milled and sintered Ti-25Nb-25Mo alloy samples were characterized systematically using x-ray diffraction, scanning electron microscope, optical microscope, and Vicker microhardness. It was noted that the quantity of the β-Ti phase increased as the milling time increased. After 12 h of milling, the synthesized alloys exhibited a spherical morphology and texture with homogeneous distribution. The milled alloys' structural evolution and morphological changes were found to be dependent on their milling duration. Morphological analysis revealed that the crystallite size and mean pore size decreased when the milling duration increased, reaching minimum values of 51 nm and < 1 μm, after 12 and 18 h respectively. As the milling time increased, the grain size decreased, resulting in an increase in density, microhardness, and elastic modulus. Ti-25Nb-25Mo will presents good anti-wear ability and higher resistance to plastic deformation due to enhanced mechanical characteristics (H/E, and H3/E2). Hence, the developed Ti-25Nb-25Mo alloys with reduced elastic modulus and desirable mechanical properties were found to be a promising option for biomedical applications.