Tributyltin alters secretion of interleukin 1 beta from human immune cells

Brown, Shyretha; Whalen, Margaret M.

doi:10.1002/jat.3087

Cited by 26 publications

(43 citation statements)

References 51 publications

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“…In fact, inhibition of p38 caused HBCD to be more effective at increasing IFNγ secretion. Previous studies have shown that p44/42 activation by TBT is involved in TBT-induced increases in IL-1β secretion (Brown & Whalen, 2014). …”

Section: Discussionmentioning

confidence: 98%

“…Secretion of IFNγ (Lawrence et al, 2015), IL-1β (Brown & Whalen, 2014), and TNF-α (Hurt et al, 2013) were all affected when immune cells were exposed to the BTs tributylin (TBT) or dibutyltin (DBT). Based on these facts, it is important to examine whether the brominated flame retardant compounds, HBCD and TBBPA, have the ability to alter the secretion of interferon gamma IFNγ from human immune cells.…”

Section: Introductionmentioning

confidence: 99%

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Hexabromocyclododecane and tetrabromobisphenol A alter secretion of interferon gamma (IFN-γ) from human immune cells

Almughamsi

Whalen

2015

Arch Toxicol

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Hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) are brominated flame retardant compounds used in a variety of applications including insulation, upholstery, and epoxy resin circuit boards. Interferon gamma (IFNγ) is an inflammatory cytokine produced by activated T and NK cells that regulates immune responsiveness. HBCD and TBBPA are found in human blood and previous studies have shown that they alter the ability of human natural killer (NK) lymphocytes to destroy tumor cells. This study examines whether HBCD and TBBPA affect the secretion of IFNγ from increasingly complex preparations of human immune cells; purified NK cells, monocyte-depleted (MD) peripheral blood mononuclear cells (PBMCs), and PBMCs. Both HBCD and TBBPA were tested at concentrations ranging from 0.05–5 μM. HBCD generally caused increases in IFNγ secretion after 24 h, 48 h, and 6 day exposures in each of the different cell preparations. The specific concentration of HBCD that caused increases as well as the magnitude of the increase varied from donor to donor. In contrast, TBBPA tended to decrease secretion of IFNγ from NK cells, MD-PBMCs and PBMCs. Thus, exposure to these compounds may potentially disrupt the immune regulation mediated by IFNγ. Signaling pathways that have the capacity to regulate IFNγ production (nuclear factor kappa B (NFκB), p44/42, p38, JNK) were examined for their role in the HBCD-induced increases in IFNγ. Results showed that the p44/42 (ERK1/2) MAPK pathway appears to be important in HBCD-induced increases in IFNγ secretion from human immune cells.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Hexabromocyclododecane and tetrabromobisphenol A alter secretion of interferon gamma (IFN-γ) from human immune cells

Almughamsi

Whalen

2015

Arch Toxicol

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“…TBBPA and HBCD have been shown to dysregulate secretion of interferon (IFN)-γ from human immune cells (Almughamsi and Whalen, 2015). Experiments with the environmental contaminant tributyltin have shown that it is able to alter secretion of IL-1β from human immune cells (Brown and Whalen, 2015). Thus, it is important to determine whether TBBPA and HBCD are able to alter secretion of IL-1β by immune cells.…”

Section: Discussionmentioning

confidence: 99%

“…In most cases, 5 μM TBBPA caused decreased IL-1β secretion by all three different cell preparations. A previous study examining the effect of TBT on secretion of IL-1β from immune system cells showed that TBT blocked IL-1β secretion at the highest concentration (200 nM) while causing increases in secretion at lower doses (5- 50 nM) (Brown and Whalen, 2015). …”

Section: Discussionmentioning

confidence: 99%

Tetrabromobisphenol A and hexabromocyclododecane alter secretion of IL-1βfrom human immune cells

Anisuzzaman

Whalen

2015

Journal of Immunotoxicology

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Tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCD), flame retardant compounds used in epoxy resin circuit boards and upholstery, contaminate the environment and are found in human serum. Lymphocytes and monocytes are immune cells that among other functions secrete pro-inflammatory cytokines such as interleukin (IL)-1β, an important regulator of immune responsiveness and tissue growth and repair. Thus, if its levels are dysregulated, loss of proper immune function and increased invasiveness of tumors could ensue. This study examines whether exposures to varying concentrations (0.05-5.0 μM) of TBBPA and HBCD for 24 hr, 48 hr, and 6 d interfere with the ability of immune cells to secrete IL-1β. The immune cell preparations examined were human natural killer (NK) cells, monocyte-depleted (MD) peripheral blood mononuclear cells (MD-PBMC) and PBMC. Both increased and decreased secretion of IL-1β from all three types of cell preparation was seen with TBBPA exposures and was dependent on concentration and length of exposure. TBBPA induced changes varied considerably from donor to donor. Exposure to HBCD from 0.5-5.0 μM caused increases in IL-1β secretion after all lengths of exposures in all cell preparations. The specific HBCD levels at which increases occurred varied among donors. Examinations of the signaling pathway(s) responsible for the elevated secretion of IL-1β after HBCD exposure were carried out in MD-PBMC cells. Results revealed that MAPK pathways (ERK1/2 and p38) appear to be the targets of HBCD that lead to increased IL-1β secretion from immune cells.

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“…Since this interleukin is pro-inflammatory material, therefore its good in stimulation both immune responses with low toxicity, which was proved by Okemoto et al, (17) they mentioned, lipid A failed to induce activation of caspase-1, which catalyzes IL-1beta precursor processing. These results suggest that an immune response without activation of caspase-1 led to lowering toxicity of this adjuvant, also IL1beta promotes cell growth, tissue repair, and immune response regulation and have a significant role in many chronic and acute inflammatory diseases (18,19). On the other hand, the decline in its concentration for other routes …”

Section: Interleukin 1βmentioning

confidence: 93%

Assessment of the Partial Purified Lipid a Extracted from Proteus mirabiliscell Wall as Adjuvant Immunotherapy

2017

IJSR

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P.mirabilis is considered one of the nosocomial infection causative agent, and its importance is related with the wide separation in different ecosystem, in addition to its relationship with renal and bile calculi ,and because of an increase of bacterial resistance, this study goal to find substitutional substance used to treat bacterial infection through using natural biomaterial, thus using LPS (Lipid A moiety) as adjuvant immunotherapy by different routes of administration(Oral, intraperitoneal, and subcutaneous) and find the best one. The results showed, recovered lipid A was obtained from both collected LPS of pathogenic P.mirabilis isolate and standard bacteria approximately represented 20% of LPS amount, with LD50 0.6 ϻg/ml for the pathogenic one. The estimation of some immune parameters such as IL-1β showed i.p administration was led to a significant elevation of this interleukin in compared with other routes and control groups. Meanwhile, IL-2, IL-10, and IFN-γ percentages showed a significant elevation in all treated groups in compared with control. While TNF-α percentage revealed variation in levels according to the route of administration, simultaneously both intraperitoneal and subcutaneous routes showed a significant elevation. Whereas the IgG level exhibited, no significant alteration observed with any treated groups in compared with control one.

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Tributyltin alters secretion of interleukin 1 beta from human immune cells

Cited by 26 publications

References 51 publications

Hexabromocyclododecane and tetrabromobisphenol A alter secretion of interferon gamma (IFN-γ) from human immune cells

Hexabromocyclododecane and tetrabromobisphenol A alter secretion of interferon gamma (IFN-γ) from human immune cells

Tetrabromobisphenol A and hexabromocyclododecane alter secretion of IL-1βfrom human immune cells

Assessment of the Partial Purified Lipid a Extracted from Proteus mirabiliscell Wall as Adjuvant Immunotherapy

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