Tributyltin induces cytogenetic damage in the early life stages of the marine mussel,Mytilus edulis

Jha, Awadhesh N.; Hagger, Josephine A.; Hill, Stephen J.

doi:10.1002/1098-2280(2000)35:4<343::aid-em9>3.0.co;2-5

Cited by 45 publications

(12 citation statements)

References 36 publications

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“…In addition to effects on the internal defence system, Jha et al (2000) found genotoxic effects of TBT on embryo-larval stages of M. edulis (sister chromatid exchanges and chromosomal aberrations). A conclusive example linking genetic damage with perturbation of endocrine function and adverse effects in vivo was provided by Hagger et al (2006).…”

Section: Antiandrogensmentioning

confidence: 98%

Endocrine disruption in prosobranch molluscs: evidence and ecological relevance

et al. 2007

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Prosobranch snails represent almost 50% of all recent molluscs, are ubiquitously distributed, play important roles in various ecosystems and exhibit a variety of reproductive modes and life-cycle-strategies. Many of them attain life spans of several years, which in combination with their limited ability to metabolize organic chemicals, may contribute to the fact that prosobranchs constitute one of the most endangered taxonomic groups in aquatic ecosystems. Although it is not yet known to what extent endocrine disrupting chemicals (EDCs) contribute to this situation, the case of tributyltin (TBT) and its population-level impact on prosobranchs demonstrates the general susceptibility of these invertebrates. The existing evidence for comparable population-level effects in prosobranch snails by other androgens, antiandrogens, and estrogens is critically reviewed. The example of TBT demonstrates the difficulty to prove an endocrine mode of action for a given chemical. Although it is generally accepted that TBT causes imposex and intersex in prosobranch snails as a result of endocrine disruption, the detailed biochemical mechanism is still a matter of debate. The strengths and weaknesses of the five competing hypotheses are discussed, together with previously unpublished data. Finally, the ecological relevance of EDC effects on the population and community level and the application of prosobranchs for the assessment of EDCs are addressed.

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Section: Antiandrogensmentioning

confidence: 98%

Endocrine disruption in prosobranch molluscs: evidence and ecological relevance

et al. 2007

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“…Following optimization and validation of the assays using the reference chemicals, the concentration range of arsenicals was selected based on earlier in vitro studies using human cells [Jha et al, 1992]. Again, the range of concentrations was slightly modified to fit a semi-logarithmic scale (i.e., 1, 3.2, and 10 M), as adopted in our earlier in vivo studies using early life stages of marine invertebrates [e.g., Jha et al, 2000]. Stock solutions of the arsenicals were prepared in culture media (without serum) and directly added to the culture flasks.…”

Section: Concentrations Of Reference Agents and Arsenicalsmentioning

confidence: 99%

Relative sensitivity of fish and mammalian cells to sodium arsenate and arsenite as determined by alkaline single‐cell gel electrophoresis and cytokinesis‐block micronucleus assay

Raisuddin

Jha

2004

Environ and Mol Mutagen

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To protect human and ecosystem health, it is necessary to develop sensitive assays and to identify responsive cells and species (and their life stages). In this study, the relative genotoxicity of two inorganic arsenicals: trivalent sodium arsenite (As(3+)) and pentavalent sodium arsenate (As(5+)), was evaluated in two cell lines of phylogenetically different origin, using alkaline single-cell gel electrophoresis (i.e., the Comet assay) and the cytokinesis-block micronucleus (MN) assay. The cell lines were the rainbow trout gonad-2 (RTG-2) and Chinese hamster ovary-K1 (CHO-K1) lines. Following optimization and validation of both assays using reference chemicals (i.e., 1-100 microM hydrogen peroxide for the Comet assay and 1-10 mM ethylmethane sulfonate for the MN assay), cells were exposed to 1-10 microM of both arsenicals to determine the relative extent of genetic damage. The unexposed controls showed similar (background) levels of damage in both cell lines and for both assays. Treatment with the arsenicals induced concentration-dependent increases in genetic damage in the two cell lines. Arsenite was more potent than arsenate in inducing DNA strand breaks in the Comet assay; at the highest concentration (10 microM) arsenite produced similar levels of DNA damage in CHO-K1 and RTG-2 cells, while 10 microM arsenate was significantly more genotoxic in RTG-2 cells. MN induction was consistently higher in RTG-2 cells than in CHO-K1 cells, with 10 microM arsenite inducing an approximate 10-fold increase in both cell lines. MN induction also was positively correlated with DNA strand breaks for both arsenicals. Overall, the study demonstrated that the fish cells are more sensitive than the mammalian cells at environmentally realistic concentrations of both arsenicals, with arsenite being more toxic.

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“…TBT increases the mortality of Crassostrea gigas embryos at 0.36 µg/L at 24 h (Tsunemasa et al, 2011), and induces malformations of Mytilus galloprovincialis embryos at 0.161 µg/L (Beiras and Bellas, 2008). TBT also induces cytogenetic damage (sister chromatid exchanges and chromosomal aberrations) in Mytilus edulis embryo (Jha et al, 2000).…”

Section: Bivalvesmentioning

confidence: 99%

Developmental toxicity of organotin compounds in animals

Chen

Zhu

et al. 2014

Front. Mar. Sci.

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Organotin compounds (OTs) have been used as biocides in antifouling paints and agriculture. The IMO introduced a global ban on the use of OTs in antifouling systems in 2001 due to their high toxicity. However, OTs have still been detected in the environment and pose a threat to the ecosystem. Several research groups have summarized the analytical methods, environmental fate, biochemistry, reproductive toxicity and mechanisms of actions of OTs. Here, we reviewed the developmental toxicity of OTs in various organisms such as sea urchin, ascidian, mussel, and fish. The differences in sensitivity to OT exposure exist not only in different species but also at different stages in the same species. Though some hypotheses have been proposed to explain the developmental toxicity of OTs, the solid evidences are greatly in need.

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Tributyltin induces cytogenetic damage in the early life stages of the marine mussel,Mytilus edulis

Cited by 45 publications

References 36 publications

Endocrine disruption in prosobranch molluscs: evidence and ecological relevance

Endocrine disruption in prosobranch molluscs: evidence and ecological relevance

Relative sensitivity of fish and mammalian cells to sodium arsenate and arsenite as determined by alkaline single‐cell gel electrophoresis and cytokinesis‐block micronucleus assay

Developmental toxicity of organotin compounds in animals

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