Tributyltin (TBT) induces oxidative stress and modifies lipid profile in the filamentous fungus Cunninghamella elegans

Abstract

To investigate the response of the tributyltin-degrading fungal strain Cunninghamella elegans to the organotin, a comparative lipidomics strategy was employed using an LC/MS-MS technique. A total of 49 lipid species were identified. Individual phospholipids were then quantified using a multiple reaction monitoring method. Tributyltin (TBT) caused a decline in the amounts of many molecular species of phosphatidylethanolamine or phosphatidylserine and an increase in the levels of phosphatidic acid, phosphatidylinositol and phosphatidylcholine. In the presence of TBT, it was observed that overall unsaturation was lower than in the control. Lipidome data were analyzed using principal component analysis, which confirmed the compositional changes in membrane lipids in response to TBT. Additionally, treatment of fungal biomass with butyltin led to a significant increase in lipid peroxidation. It is suggested that modification of the phospholipids profile and lipids peroxidation may reflect damage to mycelium caused by TBT.

Alteration of cellular lipids and lipid metabolism markers in RTL-W1 cells exposed to model endocrine disrupters

“…5), whereas those exposed to TBT had significant changes in PC and plasmalogen-PCs. TBT has been shown to disturb lipid homeostasis in the filamentous fungus Cunninghamella elegans, it led to a decrease of phosphatidylethanolamine and phosphatidylserine, but increased the levels of phosphatidic acid, phosphatidylinositol and phosphatidylcholine (Bernat et al, 2014). These changes were observed together with a decrease in the overall unsaturation of phospholipids.…”

Tributyltin (TBT) biodegradation induces oxidative stress of Cunninghamella echinulata

“…Chitin deacetylase and UDP-glucose dehydrogenase are involved in cell wall biosynthesis. The observed changes in the hyphae structure and membrane lipid composition in C. elegans during the exposure to TBT (Bernat and Długoński 2012;Bernat et al 2009b;2014a) can indicate a role of these enzymes in the TBT induced modification of cell membranes. The second antioxidant strategy examined in this work involved free amino acids analysis.…”

“…However, the nature of molecular response against stress factors induced by organotin compounds remains poorly characterized. Studies conducted on Cunninghamella elegans as a eukaryotic model revealed a number of negative effects of TBT on the organism, such as changes in the lipid profile, increased potassium retention and disturbances in the hyphae morphology (Bernat et al 2009b;Bernat and Długoński 2012;Bernat et al 2014a). Proteomics and metabolomics are useful tools for the research on the processes occurring in the cell leading to a deeper and more complex understanding of the cell behaviour under various stress or physiological conditions (Baxter and al.…”

The influence of tin ions on growth and enzymatic activity of entomopathogenic fungi

“…Although the biological functions of Sn have not been described to date, it is difficult to agree with the opinion that Sn is a nonessential metal that is of no importance to organisms [15,16]. Sn compounds affect the physiology of bacteria and fungi [17][18][19][20][21], plants [13,14], and animals [15,17,22,23]. Interactions of Sn with microorganisms are ambiguous, as although Sn and its compounds can be metabolized by some microorganisms, they are toxic to others.…”