Tributyroylglycerol hydrolase activity in Carica papaya and other latices

Giordani, R.; Moulin, A.; Verger, Robert

doi:10.1016/s0031-9422(00)95174-4

Cited by 73 publications

(80 citation statements)

References 11 publications

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“…Dentre os processos bioquímicos reportados na literatura, as lipases representam cerca de 35% dentre as enzimas empregadas 2,21 . No entanto, e especificidade (em alguns casos muito similares às microbianas), apresentam baixo custo de produção por não necessitarem de um alto grau de purificação e serem acessíveis (estão disponíveis no mercado como enzimas proteolíticas brutas) 26 . Esse trabalho tem como objetivo caracterizar as lipases vegetais presentes em látex e outras partes de plantas e que são conhecidas por suas propriedades proteolíticas, como uma forma alternativa de extração de lipases para aplicação em escala piloto e industrial.…”

Section: Figura 1 Reações Catalisadas Por Lipasesunclassified

Lipases de látex vegetais: propriedades e aplicações industriais

Paques

Macedo

2006

Quím. Nova

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Recebido em 13/10/04/; aceito em 20/4/05; publicado na web em 10/8/05PLANT LIPASES FROM LATEX: PROPERTIES AND INDUSTRIAL APPLICATIONS. Biocatalysts have innumerous advantages with respect to classical chemical processes, such as high specificity. Lipases (EC 3.1.1.3) are biocatalysts with large application in synthesis and hydrolysis reactions of triacylglycerols. The search for new sources of lipases has been intensified in the last years due to the high cost of microbial and animal lipases, wich restricts their use on an industrial scale. Lipases obtained from the latex of Carica papaya, Carica pentagona, Euphorbia characias, E. wulfenii, known for their proteolytic properties, are a good alternative source. In this review, we describe the well-known sources of vegetal lipases extracted from the latex and present some of their industrial applications.

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Section: Figura 1 Reações Catalisadas Por Lipasesunclassified

Lipases de látex vegetais: propriedades e aplicações industriais

Paques

Macedo

2006

Quím. Nova

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“…The unripe fruit of this natural hybrid (Carica stipulata Badillo and Carica pubescens Lenne and Koch [1]) exudes a latex similar to those in Carica papaya [2]. As in Carica papaya, biocatalytic activities of this latex are attributed to the presence of lipases [3,4]. In fact, these crude latex are an enzymatic cocktail with several proteases (such as papain -EC.3.4.22.2 -and chymopapain -EC.3.4.22.6 -in Carica papaya), lipases and probably other enzymes.…”

Section: Auteur(s)mentioning

confidence: 99%

Lipase activity in alcoholysis and esterification reactions of crude latex from babaco fruit (Carica pentagona)

Dhuique-Mayer

Villarreal²,

Caro

et al. 2003

OCL

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Résumé : L'activité lipasique du latex brut de la plante subtropicale de Babaco ( Carica pentagona est étudiée dans des réactions d'alcoolyse et d'estérification. Les résultats indiquent que le latex brut de Carica pentagona présente des propriétés biocatalytiques équivalentes au latex brut de Carica papaya dans des réactions de synthèse telles que l'alcoolyse de triacylglycérols et la réaction d'estérification (les rendements de réaction sont respectivement 72,3 % et 70,2 % après 3 h pour l'alcoolyse et 31,8 % et 33,8 % après 24 h pour la réaction d'estérification). Par contre, la papaïne brute commerciale donne des rendements de réaction beaucoup plus bas que les latex bruts des deux espèces de Carica (28,9 % en alcoolyse et 5,4 % en estérification).Summary : Lipase activity in alcoholysis and esterification reactions were studied for crude latex from the subtropical Babaco plant ( Carica pentagona). The results reveal that crude Carica pentagona latex exhibits equivalent biocatalytic properties in synthesis reaction via triacylglycerol alcoholysis and in esterification reaction compared to the crude latex from Carica papaya (the reaction yields are 72.3% and 70.2% respectively after 3 hrs for alcoholysis and 31.8% and 33.8% for esterification after 24 hrs). In contrast, commercially crude papain expresses lower reaction yield than crude latices from the two Carica species (28.9% in alcoholysis and 5.4% in esterification).

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“…Maximum enzyme-catalyzed yields, which correspond to 9.8% and 14.6% conversion to butyl laurate at 24h in esterification and transesterification reactions respectively, were obtained at 55°C. It is interesting to note that the catalytic activity of the enzyme was still maximal at 60°C, which indicates the relative thermal stability of the C. papaya latex lipase [16]. Nevertheless, it is recommended to work at 55°C for industrial consideration (lower energy cost).…”

Section: Effect Of the Temperature On Esterification And Transesterifmentioning

confidence: 99%

“…In contrast, the optimal activity temperature for bacterial lipases is between 37°C and 65°C with a majority being at 50-65°C [10,11]. Over the past few years, a large body of work has been reported on reactions catalyzed by plant lipases [12][13][14][15][16][17][18][19][20][21][22][23][24][25]. From an industrial viewpoint, crude plant extracts are readily available and generally are low cost materials.…”

Section: Introductionmentioning

confidence: 99%

“…From an industrial viewpoint, crude plant extracts are readily available and generally are low cost materials. Moreover, several plant lipases are obtained as naturally supported crude preparations [16]. Recent work in particular has pointed out that Carica papaya latex exhibits a powerful lipase activity for catalyzing hydrolysis [17,18], esterification [19,20] and interesterification type reactions [18,[21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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Effect of water content and temperature onCarica papayalipase catalyzed esterification and transesterification reactions

Turon¹,

Caro

Villeneuve

et al. 2003

OCL

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Summary : Temperature and water activity ( a w ) of the reaction medium are two factors that govern enzyme reactions. We studied the influence of these two parameters on the esterification and transesterification activity of Carica papaya lipase in water and solvent free reactions. It was found that over the course of reaction the catalytic activity of C. papaya lipase was dependent on these factors. The best lipase activity for both reactions was at a temperature of 55°C and water activity of 0.22, which corresponds to 2 g of water per 100 g of C. papaya latex.

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Tributyroylglycerol hydrolase activity in Carica papaya and other latices

Cited by 73 publications

References 11 publications

Lipases de látex vegetais: propriedades e aplicações industriais

Lipases de látex vegetais: propriedades e aplicações industriais

Lipase activity in alcoholysis and esterification reactions of crude latex from babaco fruit (Carica pentagona)

Effect of water content and temperature onCarica papayalipase catalyzed esterification and transesterification reactions

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