Tricalcium phosphate-based biocomposites for mandibular bone regeneration—A histological study in sheep

Russmueller, Guenter; Moser, Dominique; Spassova, E.; Plasenzotti, Roberto; Poeschl, Paul W.; Seemann, Rudolf; Becker, Stephan; Pirklbauer, Katharina; Eder-Czembirek, Christina; Czembirek, Cornelia; Perisanidis, Christos; Ewers, Rolf; Schopper, Christian

doi:10.1016/j.jcms.2015.03.022

Cited by 16 publications

(15 citation statements)

References 34 publications

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“…A similar result was reported in one study of sheep mandibular defects (Schliephake et al ., ). Another recent study in sheep, which was excluded from the present analysis because of the use of uncultured autologous bone marrow (BM; see the Supplementary material online), also reported greater regeneration in mandibular defects augmented with BM‐TCP vs. TCP‐blood constructs; this was attributed to the possible ‘osteopromotive’ effects of MSC within the BM (Russmueller et al ., ). These results are in agreement with a recent meta‐analysis of the effectiveness of stem cell therapy for histological bone regeneration in all anatomical skeletal defects of large‐animal models (Liao et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Shanbhag

Pandis

Mustafa

et al. 2016

J Tissue Eng Regen Med

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Regeneration of large, 'critical-size' bone defects remains a clinical challenge. Bone tissue engineering (BTE) is emerging as a promising alternative to autogenous, allogeneic and biomaterial-based bone grafting. The objective of this systematic review was to answer the focused question: in animal models, do cell-based BTE strategies enhance regeneration in alveolar bone critical-size defects (CSDs), compared with grafting with only biomaterial scaffolds or autogenous bone? Following PRISMA guidelines, electronic databases were searched for controlled animal studies reporting maxillary or mandibular CSD and implantation of mesenchymal stem cells (MSCs) or osteoblasts (OBs) seeded on biomaterial scaffolds. A random effects meta-analysis was performed for the outcome histomorphometric new bone formation (%NBF). Thirty-six studies were included that reported on large- (monkeys, dogs, sheep, minipigs) and small-animal (rabbits, rats) models. On average, studies presented with an unclear-to-high risk of bias and short observation times. In most studies, MSCs or OBs were used in combination with alloplastic mineral-phase scaffolds. In five studies, cells were modified by ex vivo gene transfer of bone morphogenetic proteins (BMPs). The meta-analysis indicated statistically significant benefits in favour of: (1) cell-loaded vs. cell-free scaffolds [weighted mean difference (WMD) 15.59-49.15% and 8.60-13.85% NBF in large- and small-animal models, respectively]; and (2) BMP-gene-modified vs. unmodified cells (WMD 10.06-20.83% NBF in small-animal models). Results of cell-loaded scaffolds vs. autogenous bone were inconclusive. Overall, heterogeneity in the meta-analysis was high (I > 90%). In summary, alveolar bone regeneration is enhanced by addition of osteogenic cells to biomaterial scaffolds. The direction and estimates of treatment effect are useful to predict therapeutic efficacy and guide future clinical trials of BTE. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 97%

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Shanbhag

Pandis

Mustafa

et al. 2016

J Tissue Eng Regen Med

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“…The most favorable features of bone marrow aspirate in terms of clinical application are its accessibility with little morbidity and the abundance of the cells. However bone marrow aspirates contain many osteoinductive growth factors as well as BMSCs, but are lacking an osteoconductive scaffold (17). Nowadays, it is argued that the bone marrow aspirates contains sufficient number and quality of (BMSCs) and have been shown to be effective in the treatment of bone defects, including tibial nonunions and osteonecrosis of the femoral head (10).…”

Section: Discussionmentioning

confidence: 99%

Effect of Autogenous Bone Marrow Mesenchymal Stem Cells in Healing of Mandibular Osseous Defects Grafted With Beta Tricalcium Phosphate in Dogs

Eissa

Karam

Omar

et al. 2018

Alexandria Dental Journal

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INTRODUCTION: Bone defect above critical size does not heal completely by itself and thus represents major clinical challenge to reconstructive surgery. Tissue engineering is a new approach for the repair of osseous defects. β tricalcium phosphate (β-TCP) loaded with autogenous bone marrow derived mesenchymal stem cells (BMSCs) is suggested to have a promising osteogenic characteristics that support its potential in tissue engineering to repair bone defects. OBJECTIVES: was to evaluate the efficacy of autogenous (BMSCs) by its transplantation into local defects using (β-TCP) as a carrier. MATERIALS AND METHODS: Ten male Mongrel dogs weighing 25 Kg were used in this study. Right and critical size bone defects (CSDs) were performed intraorally at the mandibular premolar area by the aid of trephine bur, of 6mm outer diameter and 5mm inner diameter. Right side bone defects (Experimental) were filled with mixture of (β-TCP) and (BMSCs) which was taken from bone marrow aspirate concentrates (BMACs) from the same animal. Meanwhile, the left side bone defects (control) were left empty for healing spontaneously. The effect of (BMSCs) was assessed histologically as well as histomorphometrically after 6 weeks. RESULTS Greater amount of bone formation was noticed in (CSDs) filled with (β-TCP) and (BMSCs). The amounts of new bone formed in the defects filled with (β-TCP) and (BMSCs) were significantly greater than those formed within the defects that were left empty. CONCLUSIONS: Autogenous (BMSCs) has excellent osteogenic characteristics and can be added to bone grafts following surgical and periodontal surgeries to enhance bone regenerative capacity.

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“…These differences in biodegradation seem to influence volume stability of regenerated bone significantly in favor of HA (Jensen et al, 2012;Russmueller et al, 2015).…”

Section: +mentioning

confidence: 99%

“…To make up for the lack of osteoinduction by these materials, a combination of bone substitutes with autologous bone or bone marrow, has been found to be beneficial (Jensen et al, 2006;Russmueller et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

Russmueller

Winkler²,

Lieber³

et al. 2017

eCM

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Much research has been done on bone cells, but only a few studies deal with biomaterial-induced effects on human osteoclasts, which may take on an important role in the successful regeneration of bone. In order to highlight such effects, human peripheral blood mononuclear cells (PBMCs) were extracted from venous blood, differentiated to osteoclasts and then cultured in, the presence of five particulate hydroxyapatite (HA)/β-tricalcium phosphate (TCP) biomaterials, on bovine bone slices and glass cover slips. The biomaterials, AlgOSS 50/50 (50 % HA/50 % TCP), AlgOSS 20/80 (20 % HA/80 % TCP), Algipore (98 % HA), Cerasorb (100 % TCP) and Bio-Oss (100 % HA) were chosen to assess their influence on cell morphology and numbers. Light microscopic evaluation was performed during ongoing cell culture. After 21 d of cultivation, the biomaterial-induced effects on osteoclastic resorption of the bone slices were evaluated by scanning electron microscopy (SEM). Osteoclast-like cells were identified by TRAP staining. All five biomaterials showed larger area fractions of resorbed bone than the control (5.6 ± 6.8 %), as measured on SEM images. The purely hydroxyapatite-based Algipore (9.8 ± 9.7 %) and Bio-Oss (7.9 ± 8.8 %) showed significantly elevated area fraction rates (p ≤ 0.05) of bone resorption. Light Microscope evaluation revealed a significant, but inhibiting effect of Cerasorb (p = 0.05). These data indicated that introducing of small biomaterial hydroxyapatite particles may have improved the performance of bone substitute materials.

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Tricalcium phosphate-based biocomposites for mandibular bone regeneration—A histological study in sheep

Cited by 16 publications

References 34 publications

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Effect of Autogenous Bone Marrow Mesenchymal Stem Cells in Healing of Mandibular Osseous Defects Grafted With Beta Tricalcium Phosphate in Dogs

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

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