2010
DOI: 10.1128/jb.01377-09
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Tricarboxylic Acid Cycle-Dependent Synthesis of Staphylococcus aureus Type 5 and 8 Capsular Polysaccharides

Abstract: Staphylococcus aureus capsule synthesis requires the precursor N-acetyl-glucosamine; however, capsule is synthesized during post-exponential growth when the availability of N-acetyl-glucosamine is limited. Capsule biosynthesis also requires aerobic respiration, leading us to hypothesize that capsule synthesis requires tricarboxylic acid cycle intermediates. Consistent with this hypothesis, S. aureus tricarboxylic acid cycle mutants fail to make capsule.Staphylococcus aureus produces two major exopolysaccharide… Show more

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Cited by 48 publications
(50 citation statements)
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“…Importantly, these data demonstrate that the effects of rpiRc inactivation on virulence determinants are independent of the genetic background. In addition to RpiRc regulating capsule accumulation, capsule biosynthesis requires TCA cycle activity (62). Consistent with high capA mRNA levels in the exponential growth phase (Fig.…”
Section: Resultsmentioning
confidence: 57%
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“…Importantly, these data demonstrate that the effects of rpiRc inactivation on virulence determinants are independent of the genetic background. In addition to RpiRc regulating capsule accumulation, capsule biosynthesis requires TCA cycle activity (62). Consistent with high capA mRNA levels in the exponential growth phase (Fig.…”
Section: Resultsmentioning
confidence: 57%
“…Capsule protects S. aureus and enhances virulence by impeding C3 complement binding and phagocytosis (74). Typically, capsule is synthesized in the postexponential growth phase, and this requires TCA cycle activity (62). As expected, capA mRNA, which encodes the first enzyme for capsule biosynthesis, increased over time in wild-type strain SA564.…”
Section: Discussionmentioning
confidence: 69%
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“…Because of this allelic organization in the chromosome, the expression of cap5 and cap8 genes is subject to similar transcriptional regulation. To date, a large number of regulators affecting cap gene transcription, some of which are non-DNA-binding factors, have been identified and/or characterized, and they include MgrA, AgrADBC, ArlRS, SaeRS, CodY, KdpDE, SigB, SpoVG, ClpC, ClpP, SbcDC, RpiRC, CcpA, Rot, CcpE, and AirSR (4)(5)(6)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”
mentioning
confidence: 99%
“…Because of this allelic organization in the chromosome, the expression of cap5 and cap8 genes is subject to similar transcriptional regulation. To date, a large number of regulators affecting cap gene transcription, some of which are non-DNA-binding factors, have been identified and/or characterized, and they include MgrA, AgrADBC, ArlRS, SaeRS, CodY, KdpDE, SigB, SpoVG, ClpC, ClpP, SbcDC, RpiRC, CcpA, Rot, CcpE,[12][13][14][15][16][17][18][19][20].Staphylococcal capsules are involved in immune evasion, but they can also mask cell surface components, such as adhesins, that are important for pathogenesis (21,22). Thus, the production of capsule must be controlled properly depending on the conditions of the environment in which S. aureus resides.…”
mentioning
confidence: 99%