Trichinella: Differential expression of angiogenic factors in macrophages stimulated with antigens from encapsulated and non-encapsulated species

Shariati, F.; Pérez-Arellano, José Luís; López‐Abán, Julio; Arefi, Maryam; Martínez-Fernández, A. R.; Muro, Antonio

doi:10.1016/j.exppara.2009.08.016

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…Division of the host muscle cell nucleus, regulation of host cell cycling, huge elevation of host collagen gene expression, and generation of new blood vessels around the collagen capsule are observed during nurse cell formation by T . spiralis [ 1 – 4 ]. The process of nurse cell formation induces de-differentiation, cell cycle re-entry, arrest of infected muscle cells, and activation, proliferation, and differentiation of satellite cells.…”

Section: Introductionmentioning

confidence: 99%

Identification of a host collagen inducing factor from the excretory secretory proteins of Trichinella spiralis

Park¹,

Kim²,

Cho³

et al. 2018

PLoS Negl Trop Dis

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BackgroundIn a previous study, we found that Trichinella spiralis muscle larva excretory and secretory proteins (ES-P) most likely activate collagen synthesis via TGF-β/Smad signaling, and this event could influence collagen capsule formation.Methodology/Principal findingsIn order to identify the specific collagen inducing factor, ES-P was fractionated by a Superdex 200 10/300 GL column. We obtained three large fractions, F1, F2, and F3, but only F3 had collagen gene inducing ability. After immunoscreening, 10 collagen inducing factor candidates were identified. Among them, TS 15–1 and TS 15–2 were identical to the putative trypsin of T. spiralis. The deduced TS 15–1 (M.W. = 72 kDa) had two conserved catalytic motifs, an N-terminal Tryp_SPc domain (TS 15-1n) and a C-terminal Tryp_SPc domain (TS 15-1c). To determine their collagen inducing ability, recombinant proteins (rTS 15-1n and rTS 15-1c) were produced using the pET-28a expression system. TS 15–1 is highly expressed during the muscle larval stage and has strong antigenicity. We determined that rTS 15-1c could elevate collagen I via activation of the TGF-β1 signaling pathway in vitro and in vivo.Conclusion/SignificanceIn conclusion, we identified a host collagen inducing factor from T. spiralis ES-P using immunoscreening and demonstrated its molecular characteristics and functions.

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Section: Introductionmentioning

confidence: 99%

Identification of a host collagen inducing factor from the excretory secretory proteins of Trichinella spiralis

Park¹,

Kim²,

Cho³

et al. 2018

PLoS Negl Trop Dis

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“…In T. spiralis infection, it was shown that VEGF was expressed in the nurse cell from day 7 up to 8 months and angiogene-sis around the nurse cell began at approximately day 12 and ceased by day 26 [1,5]. Additionally, the in vitro production of VEGF was directly stimulated by T. spiralis antigens but not by those of T. pseudospiralis [6]. A recent study showed that VEGF protein expression increased in T. spiralis-infected muscles at an early phase of infection beginning 7 days after infection and diminished after 3 weeks [7].…”

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confidence: 99%

EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR DURING NURSE CELL FORMATION IN Trichinella spiralis AND Trichinella pseudospiralis INFECTIONS

Khositharattanakool¹,

Morakote²,

SIRIAUNKGUL³

et al. 2013

Int J Parasitol Res

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IntroductionParasitic nematodes of the genus Trichinella are the etiological agents of the disease called trichinellosis which has a wide host range including birds, reptiles, several mammals as well as human. Trichinella behaves as an intracellular parasite of the striated muscle cell. There are two clades in this genus encompassing encapsulated and non-encapsulated species differentiated by the presence or absence collagen capsule around the nurse cell. T. spiralis, the most common encapsulated species, infects the muscle cell and induces remarkable changes that transform the infected muscle cell into a nurse cell that supports the growth and development of the parasite. This process is termed nurse cell formation which involves the loss of muscle proteins, enlargement and division of nurse cell nuclei, mitochondrial damage and formation of collagen [1]. Another obvious feature of the nurse cell in T. spiralis infection is the presence of a vascular network called circulatory rete surrounding it [2]. This feature probably corresponds to many observations indicating that larval and nurse cell energy metabolisms are anaerobic [3]. The anaerobic condition could be seen mimicking tissue hypoxia that stimulates angiogenesis through angiogenic factors such as vascular endothelial growth factor (VEGF) in response to hypoxic events [4]. In T. spiralis infection, it was shown that VEGF was expressed in the nurse cell from day 7 up to 8 months and angiogene-sis around the nurse cell began at approximately day 12 and ceased by day 26 [1,5]. Additionally, the in vitro production of VEGF was directly stimulated by T. spiralis antigens but not by those of T. pseudospiralis [6]. A recent study showed that VEGF protein expression increased in T. spiralis-infected muscles at an early phase of infection beginning 7 days after infection and diminished after 3 weeks [7]. The non-encapsulated T. pseudospiralis is also able to form the nurse cell but the collagen capsule, unlike in T. spiralis, is poorly developed [8,9]. Nevertheless, there has not been any study to demonstrate the presence of angiogenesis around the nurse cell of T. pseudospiralis. VEGF has been hypothesized to be involved in the development of the nurse cell-parasite complex in Trichinella infection. However, there has been so far no quantitative study of VEGF transcripts during the development of the nurse cell of both T. spiralis and T. pseudospiralis. In the present study, we attempt to determine VEGF expression in both mRNA and protein levels and localize VEGF mRNA transcript during nurse cell formation in T. spiralis and T. pseudospiralis infections.

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“…Studies performed by our group have demonstrated the induction of VEGF and FGF2 in alveolar macrophages stimulated with larvae antigens of Trichinella. spiralis (32). In the present paper, we studied the effect of somatic and excretory/secretory antigens of larvae and females of S. venezuelensis on the production of VEGF and FGF2 in alveolar macrophages.…”

Section: Discussionmentioning

confidence: 99%

Role of angiogenic factors in acute experimental Strongyloides venezuelensis infection

Shariati

Pérez-Arellano

López‐Abán

et al. 2010

Parasite Immunology

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This study aims to investigate the role of angiogenic factors in the pathogenesis of experimental strongyloidiasis. Two complementary approaches were used: Firstly, CD1 mice were treated with endostatin, an angiogenesis inhibitor, and infected with Strongyloides venezuelensis. Also, the mechanisms involved in this process were studied. Parasitological examination revealed a significant decrease in egg per gram of faeces, number of collected larvae from lung tissue and number of collected adult females in mice treated with endostatin. Direct mechanisms with diminution of angiogenesis factors and an indirect mechanism with increase of eosinophil perhaps produced their effect. Secondly, the effect of the antigens responsible for stimulation of angiogenic factors [vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2)] from alveolar macrophages and the mechanisms involved in their production were investigated. Alveolar macrophage cells obtained by bronchoalveolar lavage were incubated at different concentrations of somatic and excretory/secretory antigens of S. venezuelensis. Also, mRNA levels of VEGF and FGF2 in macrophage cells were detected by RT-PCR. L3-PBS larvae antigens induced angiogenic factors. The relationship between angiogenesis factors and nitric oxide has been observed using nitric oxide synthase inhibitors.

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Trichinella: Differential expression of angiogenic factors in macrophages stimulated with antigens from encapsulated and non-encapsulated species

Cited by 12 publications

References 28 publications

Identification of a host collagen inducing factor from the excretory secretory proteins of Trichinella spiralis

Identification of a host collagen inducing factor from the excretory secretory proteins of Trichinella spiralis

EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR DURING NURSE CELL FORMATION IN Trichinella spiralis AND Trichinella pseudospiralis INFECTIONS

Role of angiogenic factors in acute experimental Strongyloides venezuelensis infection

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