Trichinella spiralis (Owen, 1835) Induces Increased Dystrophin Expression in Invaded Cross-striated Muscle

Milcheva, Rositsa; Mečiarová, Iveta; Todorovа, Katerina; Dilcheva, V.; Petkova, S.; Babál, Pavel

doi:10.1007/s11686-023-00673-2

Cited by 1 publication

(2 citation statements)

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“…All experimental procedures concerning this chapter were already described in full detail ( Milcheva et al ., 2023 ). In brief, the expression of mRNA of mouse Nitric oxide synthase 1 ( Nos1 ) was evaluated by real-time RT-PCR in tissue samples from mouse skeletal muscle collected at 0, 14, 24, and 35 d.p.i.…”

Section: Methodsmentioning

confidence: 99%

“…The levels of expressions were estimated via normalization versus the expression of glyceraldehyde 3-phosphate dehydrogenase ( Gapdh ) as a reference gene. The infection of the samples was confirmed by end-point PCR of the Expansion Segment V (ESV) of T. spiralis ( Zarlenga et al ., 2001 ), as already described in detail ( Milcheva et al ., 2023 ). Briefly, genomic DNA from six paraffin sections from all samples was isolated using QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden Germany).…”

Section: Methodsmentioning

confidence: 99%

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Down-regulation of neuronal form of Nitric oxide synthase in the Nurse cell of Trichinella spiralis

Milcheva,

Hurníková,

Todorova

et al. 2024

Helminthologia

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Summary The free radical nitric oxide (NO) and Ca2+ are critical regulators of skeletal muscle exercise performance and fatigue. The major source of NO in skeletal muscle cells is the neuronal form of the enzyme Nitric oxide synthase (nNOS). One of the most peculiar characteristics of the Nurse cell of Trichinella spiralis (T. spiralis) is the complete loss of the contractile capabilities of its derivative striated muscle fiber. The aim of the present study was to clarify the expression of nNOS protein and mRNA in striated muscles during the muscle phase of T. spiralis infection in mice. Muscle tissue samples were collected from mice at days 0, 14, 24, and 35 post infection (d.p.i.). The expression of nNOS was investigated by immunohistochemistry, and the expression levels of mRNA of mouse Nitric oxide synthase 1 (Nos1) by real-time PCR. The presence of nNOS protein was still well observable in the disintegrated sarcoplasm at the early stage of infection. The cytoplasm of the developing and mature Nurse cell showed the absence of this protein. At least at the beginning of the Nurse cell development, Trichinella uses the same repairing process of skeletal muscle cell, induced after any trauma and this corroborates very well our results concerning the nNOS expression on day 14 p.i. At a later stage, however, we could suggest that the down-regulation of nNOS in the Nurse cell of T. spiralis either serves a protective function or is an outcome of the genetic identity of the Nurse cell.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%