Trichloroethylene mineralization in a fixed-film bioreactor using a pure culture expressing constitutively tolueneortho -monooxygenase

Abstract

An aerobic, single-pass, fixed-film bioreactor become an important compound for hazardous waste was designed for the continuous degradation and minerremediation (McFarland et al., 1992; Nyer and Moralization of gas-phase trichloroethylene (TCE). A pure cul- ello, 1993;Wilcox et al., 1995), and numerous bioture of Burkholderia cepacia PR1 23 (TOM 23C ), a Tn5 transporeactor designs have been studied for its degradation son mutant of B. cepacia G4 that constitutively expresses (Fathepure and Vogel, 1991; Folsom and Chapman, 1991; the TCE-degrading enzyme, toluene ortho-monooxygenase (TOM), was immobilized on sintered glass (SIRAN McKay et al., 1994; Phelps et al., 1990; Shields et al., carriers) and activated carbon. The inert open-pore struc-1994). The most commonly studied microorganisms are tures of the sintered glass and the strongly, TCE-ab-Methylosinus trichosporium OB3b (Brusseau et al., sorbing activated carbon provide a large surface area for

Bioreactors for treatment of VOCs and odours – A review

“…Also noteworthy, is a study by Sun and Wood (1997), who immobilized a pure culture of Burkholderia cepacia PR1 23 (TOM 23C ) constitutively expressing toluene ortho-monooxygenase to cometabolize the biodegradation of trichloroethylene (TCE) vapors in a BTF. Aerobic biodegradation of TCE only occurs through cometabolism, and addition of a growth substrate (usually toluene, methane, propane, phenol, or ammonia), which is required to induce the expression of the appropriate TCE-degrading enzyme.…”

Development and mathematical modeling of a two-stage reactor system for trichloroethylene degradation using Methylosinus trichosporium OB3b

“…The maximum volumetric degradation rate of our system was determined to be 525 mg l )1 d )1 , significantly higher TCE degradation rate than those from literatures (Sun & Wood 1997). The reactor has been stably operated more than 270 days and this system also appeared to be stable in response to the fluctuations in operation conditions such as inlet TCE concentration.…”

“…Since TCE is a suspected carcinogen and constitutes public health concern for drinking water, many efforts have been devoted to treat this hazardous waste (Alvarez-Cohen & McCarty 1991;Sun & Wood 1997).…”

Changes in soil microbial community composition induced by cometabolism of toluene and trichloroethylene

“…Degradation-mediated toxicity is attributed to breakdown products (e.g. TCE epoxides) attacking cellular components including oxygenase enzymes (Smith & McCarty 1997;Sun & Wood 1997) and is proportional to the extent of TCE co-metabolism (Alvarez-Cohen & McCarty 1991). Interestingly, standards 10 and 17 (Flavobacterium sp.…”