Trichoderma harzianum as a biocontrol agent against Alternaria alternata on tobacco

Biljana, Gveroska; Ziberoski, Jugoslav

doi:10.15208/ati.2012.9

Cited by 43 publications

(21 citation statements)

References 10 publications

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“…These results were in agreement with laboratory experiments carried out against several foliar pathogens; Hussein et al. () recorded that T. harzianum exhibited high inhibition rate of mycelial growth of Stemphylim vesicarium (the pathogen of onion leaf blight) reaching to 78.1%, Rossi and Pattori () reported that T. harzianum showed significant reduction to production of S. vesicarium conidia, and recently, Gveroska and Ziberoski () reported that T. harzianum exhibited strong reducing effect on the development of A. alternata (the pathogen of brown spot disease on tobacco) with various mechanisms of antagonistic influence.…”

Section: Discussionsupporting

confidence: 89%

Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri

Abo-Elyousr

Abdel‐Hafez

Abdel-Rahim

2014

Journal of Phytopathology

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Nine isolates of Trichoderma were collected from Assiut Governorate, Egypt, as leaf surface and endophytic fungi associated with onion flora stalks. Four isolates were identified as Trichoderma harzianum, while five isolates were belonging to Trichoderma longibrachiatum. The antagonistic activity of these isolates against onion purple blotch pathogen Alternaria porri was studied in vitro using dual culture assay. All tested Trichoderma isolates showed mycoparasitic activity and competitive capability against the mycelial growth of A. porri. Mycoparastic activity of Trichoderma was manifested morphologically by the overgrowth upon the mycelial growth of the pathogen and microscopically by production of coiling hyphae around pathogen hyphae. Isolates of T. harzianum exhibited high ability to compete on potato dextrose agar (PDA) medium causing the maximum rate of pathogen inhibition (73.12%), while isolates of T. longibrachiatum showed inhibition rate equalling 70.3%. Chitinase activity of Trichoderma was assayed, and T. harzianum Th‐3013 showed the maximum value contributing 2.69 U/min. Application of T. harzianum Th‐3013 to control purple blotch disease in vivo under greenhouse conditions caused disease reduction up to 52.3 and 79.9% before and after 48 h of pathogen inoculation, respectively, while the fungicide Ridomil Gold Plus caused disease reduction comprising 56.5 and 71.7%, respectively. This study proved that T. harzianum Th‐3013 as a biocontrol agent showed significant reduction in onion purple blotch disease compared with the tested fungicide.

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Section: Discussionsupporting

confidence: 89%

Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri

Abo-Elyousr

Abdel‐Hafez

Abdel-Rahim

2014

Journal of Phytopathology

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“…T. harzianum develops without obstacles, and it has opportunities to stop the development of A. alternate. It grows over the colony of the pathogen at the same time (Biljana and Jugosslave, 2011;Gveroska and Ziberosk, 2011). Rajendiran et al (2010) demonstrated the inhibitory effect of Trichoderma viride against Fusarium sp., Penicillium sp.…”

Section: Discussionmentioning

confidence: 99%

In vitro antagonistic activity evaluation of some selected fungi isolated from burned soils in Mila region (East of Algeria)

Abdelaziz¹,

Khiat²,

Khiat³

2016

Afr. J. Microbiol. Res.

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The present study was initiated to (i) determine burned forest-inhabiting fungi in Zouagha, TerriBeinène, Mila and (ii) study the antagonistic activities of Trichoderma sp against Fusarium sp, Penicillium sp, Rhizoctonia sp, and Alternaria sp. Eighteen fungal strains representing six genera were isolated from soil samples obtained from the burned forest of Zouagha in the Mila region: Trichoderma sp, Fusarium sp, Penicillium sp, Rhizoctonia sp, Alternaria sp, and Rhizopus sp. The direct antagonistic activity assays of Trichoderma sp on Potato Dextrose Agar medium (PDA) against the four fungi: Fusarium sp, Penicillium sp, Rhizoctonia sp, and Alternaria sp revealed that the fungus Trichoderma sp reduced the mycelium growth of Fusarium sp, Penicillium sp, Rhizoctonia sp and Alternaria sp to 23.13, 33.13, 33.75, and 38.31%, respectively, compared to the control after six days at room temperature. The results illustrated an inhibitory action of the antagonist Trichoderma sp characterized by slowing the mycelial growth of fungal strains. Strains of Fusarium sp, Penicillium sp, Rhizoctonia sp and Alternaria sp showed differences in the sensitivity to the antagonist. Because Trichoderma occurred more frequently in burned soils and were more antagonistic to phytopathogenic fungi in culture than isolates from unburned soils, the judicious use of fire may increase the abundance of Trichoderma isolates and their inhibitory action may be used for the control of fungal plant diseases.

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“…In this respect, several reseachers have reported that Trichoderma has a good antagonistic effect on mycelial growth and conidial germination of Alternaria porri (Imtiaj & Lee 2008). Trichoderma harzianum exhibited a strong suppressive effect on the development of many foliar plant pathogens such as Alternaria alternata and Stemphylium vesicarium causing high inhibition of mycelial growth and conidial germination (Gveroska & Ziberoski 2012, Hussein et al 2007, Rossi & Pattori 2009.…”

Section: Antagonistic Effect Of Detected Species Against Pathogenic Fmentioning

confidence: 99%

Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri.

Abdel‐Hafez¹,

Abo-Elyousr²,

Abdel-Rahim³

2015

Czech Mycol.

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Sixty-eight fungal species belonging to 29 genera were isolated as leaf surface and endophytic fungi from healthy and purple blotch diseased onion leaves. The fungal populations associated with diseased onion leaves (1.360 × 10 3 CFU/g leaf in the phyllosphere, 2.614 CFU/leaf segment in the phylloplane and 1.324 CFU/leaf segment in the surface-sterilised diseased leaves) were higher than those in healthy samples (0.804 × 10 3 CFU in the phyllosphere, 1.184 CFU in the phylloplane, and 0.35 CFU as endophytes). Endophytic fungi of healthy leaves were represented by 12 genera and 15 species, while fungi of surface-sterilised diseased leaves included 17 species from 13 genera. The mycobiota associated with surface-sterilised diseased leaves were different from the endophytic fungi of healthy samples, whereas the disease may stimulate colonisation of opportunistic fungi causing secondary infections such as Botrytis cinerea, Penicillium aurantiogriseum, Alternaria alternata and Cladosporium spp. In contrast, healthy leaves were a source of antagonistic endophytic fungi such as Trichoderma harzianum and T. koningii. Testing the antagonistic effect of 91 fungal isolates against Alternaria porri showed that nine isolates of Trichoderma produced the highest suppressive potential (73.1%) depending on competition and mycoparasitism. Epicoccum nigrum and Penicillium oxalicum exhibited antibiosis against A. porri producing a 12 mm broad inhibition zone. In conclusion, the quantitative and qualitative compositions of fungi associated with onion leaves were distinctly influenced by A. porri infection. Mycobiota associated with asymptomatic onion leaves such as Epicoccum nigrum, Penicillium oxalicum and Trichoderma harzianum are a natural source of eco-friendly bioagents. They showed an effective antagonistic potential against A. porri, and may thus be applied as an alternative to fungicides.

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Trichoderma harzianum as a biocontrol agent against Alternaria alternata on tobacco

Cited by 43 publications

References 10 publications

Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri

Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri

In vitro antagonistic activity evaluation of some selected fungi isolated from burned soils in Mila region (East of Algeria)

Leaf surface and endophytic fungi associated with onion leaves and their antagonistic activity against Alternaria porri.

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